Utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin I and their applications involving animal models
Abstract This study develops an ultrasensitive electrical device, the silicon nanowire-field effect transistor (SiNW-FET) for detection of cardiac troponin I (cTnI) in obesity induced myocardial injury. The biosensor device utilizes metal–oxide–semiconductor (MOS) compatible top-down methodology for...
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doaj-3609e10ae46d4a9fbbbd58aa3054cceb2020-12-20T12:33:52ZengNature Publishing GroupScientific Reports2045-23222020-12-0110111110.1038/s41598-020-78829-7Utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin I and their applications involving animal modelsShih-Mein Chang0Sathyadevi Palanisamy1Tung-Ho Wu2Chiao-Yun Chen3Kai-Hung Cheng4Chen-Yi Lee5Shyng-Shiou F. Yuan6Yun-Ming Wang7Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-Devices (IDS2B), National Chiao Tung UniversityDepartment of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-Devices (IDS2B), National Chiao Tung UniversityDivision of Cardiovascular Surgery, Department of Surgery and Division of Surgical Critical Care, Department of Critical Care Medicine, Veterans General HospitalDepartment of Radiology, Faculty of Medicine, College of Medicine, Kaohsiung Medical UniversityDivision of Cardiology, Department of Internal Medicine, Kaohsiung Medical University HospitalDepartment of Electronics Engineering, National Chiao Tung UniversityTranslational Research Center, Kaohsiung Medical University Hospital, Kaohsiung Medical UniversityDepartment of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-Devices (IDS2B), National Chiao Tung UniversityAbstract This study develops an ultrasensitive electrical device, the silicon nanowire-field effect transistor (SiNW-FET) for detection of cardiac troponin I (cTnI) in obesity induced myocardial injury. The biosensor device utilizes metal–oxide–semiconductor (MOS) compatible top-down methodology for the fabrication process. After fabrication, the surface of the SiNW is modified with the cTnI monoclonal antibody (Mab-cTnI) upon covalent immobilization to capture cTnI antigen. The sensitivity of the device is also examined using cTnI at different concentrations with the lowest detection limit of 0.016 ng/mL. The electrocardiogram (ECG), magnetic resonance imaging (MRI), and superior vena cave (SVC) provide more information about cardiac responses in a mouse model of acute myocardial infarction (AMI). Further, magnetic resonance imaging helps to evaluate the cardiac output of an obesity induced myocardial injury mouse model. These methods play an essential role in monitoring the obesity based cardiac injury and hence, these studies were carried out. This is the first report to use the ECG, MRI, and SVC sampling methods to study the obesity based cardiac injury involving Syrian hamsters as animal models. The proposed SiNW-FET in this study shows greater sensitivity than the previously developed devices and demonstrates great potential for future applications in point-of-care (POC) diagnosis.https://doi.org/10.1038/s41598-020-78829-7 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shih-Mein Chang Sathyadevi Palanisamy Tung-Ho Wu Chiao-Yun Chen Kai-Hung Cheng Chen-Yi Lee Shyng-Shiou F. Yuan Yun-Ming Wang |
spellingShingle |
Shih-Mein Chang Sathyadevi Palanisamy Tung-Ho Wu Chiao-Yun Chen Kai-Hung Cheng Chen-Yi Lee Shyng-Shiou F. Yuan Yun-Ming Wang Utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin I and their applications involving animal models Scientific Reports |
author_facet |
Shih-Mein Chang Sathyadevi Palanisamy Tung-Ho Wu Chiao-Yun Chen Kai-Hung Cheng Chen-Yi Lee Shyng-Shiou F. Yuan Yun-Ming Wang |
author_sort |
Shih-Mein Chang |
title |
Utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin I and their applications involving animal models |
title_short |
Utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin I and their applications involving animal models |
title_full |
Utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin I and their applications involving animal models |
title_fullStr |
Utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin I and their applications involving animal models |
title_full_unstemmed |
Utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin I and their applications involving animal models |
title_sort |
utilization of silicon nanowire field-effect transistors for the detection of a cardiac biomarker, cardiac troponin i and their applications involving animal models |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2020-12-01 |
description |
Abstract This study develops an ultrasensitive electrical device, the silicon nanowire-field effect transistor (SiNW-FET) for detection of cardiac troponin I (cTnI) in obesity induced myocardial injury. The biosensor device utilizes metal–oxide–semiconductor (MOS) compatible top-down methodology for the fabrication process. After fabrication, the surface of the SiNW is modified with the cTnI monoclonal antibody (Mab-cTnI) upon covalent immobilization to capture cTnI antigen. The sensitivity of the device is also examined using cTnI at different concentrations with the lowest detection limit of 0.016 ng/mL. The electrocardiogram (ECG), magnetic resonance imaging (MRI), and superior vena cave (SVC) provide more information about cardiac responses in a mouse model of acute myocardial infarction (AMI). Further, magnetic resonance imaging helps to evaluate the cardiac output of an obesity induced myocardial injury mouse model. These methods play an essential role in monitoring the obesity based cardiac injury and hence, these studies were carried out. This is the first report to use the ECG, MRI, and SVC sampling methods to study the obesity based cardiac injury involving Syrian hamsters as animal models. The proposed SiNW-FET in this study shows greater sensitivity than the previously developed devices and demonstrates great potential for future applications in point-of-care (POC) diagnosis. |
url |
https://doi.org/10.1038/s41598-020-78829-7 |
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