A simple and accurate two-step long DNA sequences synthesis strategy to improve heterologous gene expression in pichia.
In vitro gene chemical synthesis is a powerful tool to improve the expression of gene in heterologous system. In this study, a two-step gene synthesis strategy that combines an assembly PCR and an overlap extension PCR (AOE) was developed. In this strategy, the chemically synthesized oligonucleotide...
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Public Library of Science (PLoS)