A simple and accurate two-step long DNA sequences synthesis strategy to improve heterologous gene expression in pichia.

In vitro gene chemical synthesis is a powerful tool to improve the expression of gene in heterologous system. In this study, a two-step gene synthesis strategy that combines an assembly PCR and an overlap extension PCR (AOE) was developed. In this strategy, the chemically synthesized oligonucleotide...

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Bibliographic Details
Main Authors: Jiang-Ke Yang, Fang-Yuan Chen, Xiang-Xiang Yan, Li-Hong Miao, Jiang-Hong Dai
Format: Article
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3344903?pdf=render