Morpho-Functional Consequences of <i>Swiss Cheese</i> Knockdown in Glia of <i>Drosophila melanogaster</i>
Glia are crucial for the normal development and functioning of the nervous system in many animals. Insects are widely used for studies of glia genetics and physiology. <i>Drosophila melanogaster</i> surface glia (perineurial and subperineurial) form a blood–brain barrier in the central n...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-03-01
|
Series: | Cells |
Subjects: | |
Online Access: | https://www.mdpi.com/2073-4409/10/3/529 |
id |
doaj-367815fd467a42e5878e28143fd6c021 |
---|---|
record_format |
Article |
spelling |
doaj-367815fd467a42e5878e28143fd6c0212021-03-03T00:03:34ZengMDPI AGCells2073-44092021-03-011052952910.3390/cells10030529Morpho-Functional Consequences of <i>Swiss Cheese</i> Knockdown in Glia of <i>Drosophila melanogaster</i>Elena V. Ryabova0Pavel A. Melentev1Artem E. Komissarov2Nina V. Surina3Ekaterina A. Ivanova4Natalia Matiytsiv5Halyna R. Shcherbata6Svetlana V. Sarantseva7Molecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of NRC «Kurchatov Institute», 188300 Gatchina, RussiaMolecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of NRC «Kurchatov Institute», 188300 Gatchina, RussiaMolecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of NRC «Kurchatov Institute», 188300 Gatchina, RussiaMolecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of NRC «Kurchatov Institute», 188300 Gatchina, RussiaMolecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of NRC «Kurchatov Institute», 188300 Gatchina, RussiaDepartment of Genetics and Biotechnology, Faculty of Biology, Ivan Franko Lviv National University, 79005 Lviv, UkraineInstitute of Cell Biochemistry, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, GermanyMolecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of NRC «Kurchatov Institute», 188300 Gatchina, RussiaGlia are crucial for the normal development and functioning of the nervous system in many animals. Insects are widely used for studies of glia genetics and physiology. <i>Drosophila melanogaster</i> surface glia (perineurial and subperineurial) form a blood–brain barrier in the central nervous system and blood–nerve barrier in the peripheral nervous system. Under the subperineurial glia layer, in the cortical region of the central nervous system, cortex glia encapsulate neuronal cell bodies, whilst in the peripheral nervous system, wrapping glia ensheath axons of peripheral nerves. Here, we show that the expression of the evolutionarily conserved <i>swiss cheese</i> gene is important in several types of glia. <i>swiss cheese</i> knockdown in subperineurial glia leads to morphological abnormalities of these cells. We found that the number of subperineurial glia nuclei is reduced under <i>swiss cheese</i> knockdown, possibly due to apoptosis. In addition, the downregulation of <i>swiss cheese</i> in wrapping glia causes a loss of its integrity. We reveal transcriptome changes under <i>swiss cheese</i> knockdown in subperineurial glia and in cortex + wrapping glia and show that the downregulation of <i>swiss cheese</i> in these types of glia provokes reactive oxygen species acceleration. These results are accompanied by a decline in animal mobility measured by the negative geotaxis performance assay.https://www.mdpi.com/2073-4409/10/3/529<i>swiss cheese</i><i>NTE</i>PNPLA6Drosophila melanogastergliablood–brain barrier |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elena V. Ryabova Pavel A. Melentev Artem E. Komissarov Nina V. Surina Ekaterina A. Ivanova Natalia Matiytsiv Halyna R. Shcherbata Svetlana V. Sarantseva |
spellingShingle |
Elena V. Ryabova Pavel A. Melentev Artem E. Komissarov Nina V. Surina Ekaterina A. Ivanova Natalia Matiytsiv Halyna R. Shcherbata Svetlana V. Sarantseva Morpho-Functional Consequences of <i>Swiss Cheese</i> Knockdown in Glia of <i>Drosophila melanogaster</i> Cells <i>swiss cheese</i> <i>NTE</i> PNPLA6 Drosophila melanogaster glia blood–brain barrier |
author_facet |
Elena V. Ryabova Pavel A. Melentev Artem E. Komissarov Nina V. Surina Ekaterina A. Ivanova Natalia Matiytsiv Halyna R. Shcherbata Svetlana V. Sarantseva |
author_sort |
Elena V. Ryabova |
title |
Morpho-Functional Consequences of <i>Swiss Cheese</i> Knockdown in Glia of <i>Drosophila melanogaster</i> |
title_short |
Morpho-Functional Consequences of <i>Swiss Cheese</i> Knockdown in Glia of <i>Drosophila melanogaster</i> |
title_full |
Morpho-Functional Consequences of <i>Swiss Cheese</i> Knockdown in Glia of <i>Drosophila melanogaster</i> |
title_fullStr |
Morpho-Functional Consequences of <i>Swiss Cheese</i> Knockdown in Glia of <i>Drosophila melanogaster</i> |
title_full_unstemmed |
Morpho-Functional Consequences of <i>Swiss Cheese</i> Knockdown in Glia of <i>Drosophila melanogaster</i> |
title_sort |
morpho-functional consequences of <i>swiss cheese</i> knockdown in glia of <i>drosophila melanogaster</i> |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2021-03-01 |
description |
Glia are crucial for the normal development and functioning of the nervous system in many animals. Insects are widely used for studies of glia genetics and physiology. <i>Drosophila melanogaster</i> surface glia (perineurial and subperineurial) form a blood–brain barrier in the central nervous system and blood–nerve barrier in the peripheral nervous system. Under the subperineurial glia layer, in the cortical region of the central nervous system, cortex glia encapsulate neuronal cell bodies, whilst in the peripheral nervous system, wrapping glia ensheath axons of peripheral nerves. Here, we show that the expression of the evolutionarily conserved <i>swiss cheese</i> gene is important in several types of glia. <i>swiss cheese</i> knockdown in subperineurial glia leads to morphological abnormalities of these cells. We found that the number of subperineurial glia nuclei is reduced under <i>swiss cheese</i> knockdown, possibly due to apoptosis. In addition, the downregulation of <i>swiss cheese</i> in wrapping glia causes a loss of its integrity. We reveal transcriptome changes under <i>swiss cheese</i> knockdown in subperineurial glia and in cortex + wrapping glia and show that the downregulation of <i>swiss cheese</i> in these types of glia provokes reactive oxygen species acceleration. These results are accompanied by a decline in animal mobility measured by the negative geotaxis performance assay. |
topic |
<i>swiss cheese</i> <i>NTE</i> PNPLA6 Drosophila melanogaster glia blood–brain barrier |
url |
https://www.mdpi.com/2073-4409/10/3/529 |
work_keys_str_mv |
AT elenavryabova morphofunctionalconsequencesofiswisscheeseiknockdowningliaofidrosophilamelanogasteri AT pavelamelentev morphofunctionalconsequencesofiswisscheeseiknockdowningliaofidrosophilamelanogasteri AT artemekomissarov morphofunctionalconsequencesofiswisscheeseiknockdowningliaofidrosophilamelanogasteri AT ninavsurina morphofunctionalconsequencesofiswisscheeseiknockdowningliaofidrosophilamelanogasteri AT ekaterinaaivanova morphofunctionalconsequencesofiswisscheeseiknockdowningliaofidrosophilamelanogasteri AT nataliamatiytsiv morphofunctionalconsequencesofiswisscheeseiknockdowningliaofidrosophilamelanogasteri AT halynarshcherbata morphofunctionalconsequencesofiswisscheeseiknockdowningliaofidrosophilamelanogasteri AT svetlanavsarantseva morphofunctionalconsequencesofiswisscheeseiknockdowningliaofidrosophilamelanogasteri |
_version_ |
1724233850704363520 |