Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor
The detection of CA 125 protein in a solution using a silicon-on-insulator (SOI)-nanowire biosensor with n-type chip has been experimentally demonstrated. The surface of nanowires was modified by covalent immobilization of antibodies against CA 125 in order to provide the biospecificity of the targe...
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MDPI AG
2020-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/10/12/210 |
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doaj-3709f3ca25734db1afcb6c49f88eb6f62020-12-19T00:06:38ZengMDPI AGBiosensors2079-63742020-12-011021021010.3390/bios10120210Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire BiosensorKristina A. Malsagova0Tatyana O. Pleshakova1Rafael A. Galiullin2Andrey F. Kozlov3Ivan D. Shumov4Vladimir P. Popov5Fedor V. Tikhonenko6Alexander V. Glukhov7Vadim S. Ziborov8Oleg F. Petrov9Vladimir E. Fortov10Alexander I. Archakov11Yuri D. Ivanov12Laboratory of Nanotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaLaboratory of Nanotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaLaboratory of Nanotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaLaboratory of Nanotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaLaboratory of Nanotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaRzhanov Institute of Semiconductor Physics, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaRzhanov Institute of Semiconductor Physics, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, RussiaJSC Novosibirsk Plant of Semiconductor Devices with OKB, 630082 Novosibirsk, RussiaLaboratory of Nanotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaJoint Institute for High Temperatures of Russian Academy of Sciences, 125412 Moscow, RussiaJoint Institute for High Temperatures of Russian Academy of Sciences, 125412 Moscow, RussiaLaboratory of Nanotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaLaboratory of Nanotechnology, Institute of Biomedical Chemistry, 119121 Moscow, RussiaThe detection of CA 125 protein in a solution using a silicon-on-insulator (SOI)-nanowire biosensor with n-type chip has been experimentally demonstrated. The surface of nanowires was modified by covalent immobilization of antibodies against CA 125 in order to provide the biospecificity of the target protein detection. We have demonstrated that the biosensor signal, which results from the biospecific interaction between CA 125 and the covalently immobilized antibodies, increases with the increase in the protein concentration. At that, the minimum concentration, at which the target protein was detectable with the SOI-nanowire biosensor, amounted to 1.5 × 10<sup>−16</sup> М.https://www.mdpi.com/2079-6374/10/12/210ovarian cancernanowire biosensornanowiresilicon-on-insulatorCA125antibodies |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Kristina A. Malsagova Tatyana O. Pleshakova Rafael A. Galiullin Andrey F. Kozlov Ivan D. Shumov Vladimir P. Popov Fedor V. Tikhonenko Alexander V. Glukhov Vadim S. Ziborov Oleg F. Petrov Vladimir E. Fortov Alexander I. Archakov Yuri D. Ivanov |
| spellingShingle |
Kristina A. Malsagova Tatyana O. Pleshakova Rafael A. Galiullin Andrey F. Kozlov Ivan D. Shumov Vladimir P. Popov Fedor V. Tikhonenko Alexander V. Glukhov Vadim S. Ziborov Oleg F. Petrov Vladimir E. Fortov Alexander I. Archakov Yuri D. Ivanov Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor Biosensors ovarian cancer nanowire biosensor nanowire silicon-on-insulator CA125 antibodies |
| author_facet |
Kristina A. Malsagova Tatyana O. Pleshakova Rafael A. Galiullin Andrey F. Kozlov Ivan D. Shumov Vladimir P. Popov Fedor V. Tikhonenko Alexander V. Glukhov Vadim S. Ziborov Oleg F. Petrov Vladimir E. Fortov Alexander I. Archakov Yuri D. Ivanov |
| author_sort |
Kristina A. Malsagova |
| title |
Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor |
| title_short |
Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor |
| title_full |
Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor |
| title_fullStr |
Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor |
| title_full_unstemmed |
Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor |
| title_sort |
highly sensitive detection of ca 125 protein with the use of an n-type nanowire biosensor |
| publisher |
MDPI AG |
| series |
Biosensors |
| issn |
2079-6374 |
| publishDate |
2020-12-01 |
| description |
The detection of CA 125 protein in a solution using a silicon-on-insulator (SOI)-nanowire biosensor with n-type chip has been experimentally demonstrated. The surface of nanowires was modified by covalent immobilization of antibodies against CA 125 in order to provide the biospecificity of the target protein detection. We have demonstrated that the biosensor signal, which results from the biospecific interaction between CA 125 and the covalently immobilized antibodies, increases with the increase in the protein concentration. At that, the minimum concentration, at which the target protein was detectable with the SOI-nanowire biosensor, amounted to 1.5 × 10<sup>−16</sup> М. |
| topic |
ovarian cancer nanowire biosensor nanowire silicon-on-insulator CA125 antibodies |
| url |
https://www.mdpi.com/2079-6374/10/12/210 |
| work_keys_str_mv |
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