Lack of Gut Secretory Immunoglobulin A in Memory B-Cell Dysfunction-Associated Disorders: A Possible Gut-Spleen Axis
Background: B-1a B cells and gut secretory IgA (SIgA) are absent in asplenic mice. Human immunoglobulin M (IgM) memory B cells, which are functionally equivalent to mouse B-1a B cells, are reduced after splenectomy.Objective: To demonstrate whether IgM memory B cells are necessary for generating IgA...
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doaj-37f68843b63841d29b064d7051d291f32020-11-25T02:45:39ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-01-011010.3389/fimmu.2019.02937487554Lack of Gut Secretory Immunoglobulin A in Memory B-Cell Dysfunction-Associated Disorders: A Possible Gut-Spleen AxisRita Carsetti0Rita Carsetti1Antonio Di Sabatino2Maria Manuela Rosado3Simona Cascioli4Eva Piano Mortari5Cinzia Milito6Ola Grimsholm7Alaitz Aranburu8Ezio Giorda9Francesco Paolo Tinozzi10Federica Pulvirenti11Giuseppe Donato12Francesco Morini13Pietro Bagolan14Gino Roberto Corazza15Isabella Quinti16B Cell Pathophysiology Unit, IRCCS Bambino Gesù Children's Hospital, Rome, ItalyDiagnostic Immunology Unit, IRCCS Bambino Gesù Children's Hospital, Rome, ItalyFirst Department of Medicine, IRCCS San Matteo Hospital Foundation, University of Pavia, Pavia, ItalyB Cell Pathophysiology Unit, IRCCS Bambino Gesù Children's Hospital, Rome, ItalyB Cell Pathophysiology Unit, IRCCS Bambino Gesù Children's Hospital, Rome, ItalyB Cell Pathophysiology Unit, IRCCS Bambino Gesù Children's Hospital, Rome, ItalyDepartment of Molecular Medicine, Sapienza University, Rome, ItalyB Cell Pathophysiology Unit, IRCCS Bambino Gesù Children's Hospital, Rome, ItalyB Cell Pathophysiology Unit, IRCCS Bambino Gesù Children's Hospital, Rome, ItalyB Cell Pathophysiology Unit, IRCCS Bambino Gesù Children's Hospital, Rome, ItalySecond Department of Surgery, IRCCS San Matteo Hospital Foundation, University of Pavia, Pavia, ItalyDepartment of Molecular Medicine, Sapienza University, Rome, ItalyDepartment of Translational and Precision Medicine, Sapienza University, Rome, ItalyDepartment of Medical and Surgical Neonatology, IRCCS Bambino Gesù Children's Hospital, Rome, ItalyDepartment of Medical and Surgical Neonatology, IRCCS Bambino Gesù Children's Hospital, Rome, ItalyFirst Department of Medicine, IRCCS San Matteo Hospital Foundation, University of Pavia, Pavia, ItalyDepartment of Molecular Medicine, Sapienza University, Rome, ItalyBackground: B-1a B cells and gut secretory IgA (SIgA) are absent in asplenic mice. Human immunoglobulin M (IgM) memory B cells, which are functionally equivalent to mouse B-1a B cells, are reduced after splenectomy.Objective: To demonstrate whether IgM memory B cells are necessary for generating IgA-secreting plasma cells in the human gut.Methods: We studied intestinal SIgA in two disorders sharing the IgM memory B cell defect, namely asplenia, and common variable immune deficiency (CVID).Results: Splenectomy was associated with reduced circulating IgM memory B cells and disappearance of intestinal IgA-secreting plasma cells. CVID patients with reduced circulating IgM memory B cells had a reduced frequency of gut IgA+ plasma cells and a disrupted film of SIgA on epithelial cells. Toll-like receptor 9 (TLR9) and transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI) induced IgM memory B cell differentiation into IgA+ plasma cells in vitro. In the human gut, TACI-expressing IgM memory B cells were localized under the epithelial cell layer where the TACI ligand a proliferation inducing ligand (APRIL) was extremely abundant.Conclusions: Circulating IgM memory B cell depletion was associated with a defect of intestinal IgA-secreting plasma cells in asplenia and CVID. The observation that IgM memory B cells have a distinctive role in mucosal protection suggests the existence of a functional gut-spleen axis.https://www.frontiersin.org/article/10.3389/fimmu.2019.02937/fullcommon variable immune deficiencygut mucosal immunologyplasma cellsplenectomytransmembrane activator and calcium-modulator and cyclophilin ligand interactor |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rita Carsetti Rita Carsetti Antonio Di Sabatino Maria Manuela Rosado Simona Cascioli Eva Piano Mortari Cinzia Milito Ola Grimsholm Alaitz Aranburu Ezio Giorda Francesco Paolo Tinozzi Federica Pulvirenti Giuseppe Donato Francesco Morini Pietro Bagolan Gino Roberto Corazza Isabella Quinti |
spellingShingle |
Rita Carsetti Rita Carsetti Antonio Di Sabatino Maria Manuela Rosado Simona Cascioli Eva Piano Mortari Cinzia Milito Ola Grimsholm Alaitz Aranburu Ezio Giorda Francesco Paolo Tinozzi Federica Pulvirenti Giuseppe Donato Francesco Morini Pietro Bagolan Gino Roberto Corazza Isabella Quinti Lack of Gut Secretory Immunoglobulin A in Memory B-Cell Dysfunction-Associated Disorders: A Possible Gut-Spleen Axis Frontiers in Immunology common variable immune deficiency gut mucosal immunology plasma cell splenectomy transmembrane activator and calcium-modulator and cyclophilin ligand interactor |
author_facet |
Rita Carsetti Rita Carsetti Antonio Di Sabatino Maria Manuela Rosado Simona Cascioli Eva Piano Mortari Cinzia Milito Ola Grimsholm Alaitz Aranburu Ezio Giorda Francesco Paolo Tinozzi Federica Pulvirenti Giuseppe Donato Francesco Morini Pietro Bagolan Gino Roberto Corazza Isabella Quinti |
author_sort |
Rita Carsetti |
title |
Lack of Gut Secretory Immunoglobulin A in Memory B-Cell Dysfunction-Associated Disorders: A Possible Gut-Spleen Axis |
title_short |
Lack of Gut Secretory Immunoglobulin A in Memory B-Cell Dysfunction-Associated Disorders: A Possible Gut-Spleen Axis |
title_full |
Lack of Gut Secretory Immunoglobulin A in Memory B-Cell Dysfunction-Associated Disorders: A Possible Gut-Spleen Axis |
title_fullStr |
Lack of Gut Secretory Immunoglobulin A in Memory B-Cell Dysfunction-Associated Disorders: A Possible Gut-Spleen Axis |
title_full_unstemmed |
Lack of Gut Secretory Immunoglobulin A in Memory B-Cell Dysfunction-Associated Disorders: A Possible Gut-Spleen Axis |
title_sort |
lack of gut secretory immunoglobulin a in memory b-cell dysfunction-associated disorders: a possible gut-spleen axis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2020-01-01 |
description |
Background: B-1a B cells and gut secretory IgA (SIgA) are absent in asplenic mice. Human immunoglobulin M (IgM) memory B cells, which are functionally equivalent to mouse B-1a B cells, are reduced after splenectomy.Objective: To demonstrate whether IgM memory B cells are necessary for generating IgA-secreting plasma cells in the human gut.Methods: We studied intestinal SIgA in two disorders sharing the IgM memory B cell defect, namely asplenia, and common variable immune deficiency (CVID).Results: Splenectomy was associated with reduced circulating IgM memory B cells and disappearance of intestinal IgA-secreting plasma cells. CVID patients with reduced circulating IgM memory B cells had a reduced frequency of gut IgA+ plasma cells and a disrupted film of SIgA on epithelial cells. Toll-like receptor 9 (TLR9) and transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI) induced IgM memory B cell differentiation into IgA+ plasma cells in vitro. In the human gut, TACI-expressing IgM memory B cells were localized under the epithelial cell layer where the TACI ligand a proliferation inducing ligand (APRIL) was extremely abundant.Conclusions: Circulating IgM memory B cell depletion was associated with a defect of intestinal IgA-secreting plasma cells in asplenia and CVID. The observation that IgM memory B cells have a distinctive role in mucosal protection suggests the existence of a functional gut-spleen axis. |
topic |
common variable immune deficiency gut mucosal immunology plasma cell splenectomy transmembrane activator and calcium-modulator and cyclophilin ligand interactor |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2019.02937/full |
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