Mutational Analysis of Ocriplasmin to Reduce Proteolytic and Autolytic Activity in Pichia pastoris

Mutational Analysis of Ocriplasmin to Reduce Proteolytic and Autolytic Activity in Pichia pastoris

Abstract Background Ocriplasmin (Jetrea) is using for the treatment of symptomatic vitreomacular adhesion. This enzyme undergoes rapid inactivation and limited activity duration as a result of its autolytic nature after injection within the eye. Moreover, the proteolytic activity can cause photorece...

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Bibliographic Details
Main Authors: Roghayyeh Baghban, Safar Farajnia, Younes Ghasemi, Reyhaneh Hoseinpoor, Azam Safary, Mojtaba Mortazavi, Nosratollah Zarghami
Format: Article
Language:English
Published: BMC 2020-12-01
Series:Biological Procedures Online
Subjects:
Ocriplasmin
Pichia pastoris
Site-directed mutagenesis
Proteolytic activity
Autolytic activity
Online Access:https://doi.org/10.1186/s12575-020-00138-0

Internet

https://doi.org/10.1186/s12575-020-00138-0

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