The structural basis for the integrity of adenovirus Ad3 dodecahedron.
During the viral life cycle adenoviruses produce excess capsid proteins. Human adenovirus serotype 3 (Ad3) synthesizes predominantly an excess of free pentons, the complexes of pentameric penton base and trimeric fiber proteins, which are responsible for virus penetration. In infected cells Ad3 pent...
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doaj-394de866805e45adbb6eae6cefdfc3082020-11-24T21:49:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4607510.1371/journal.pone.0046075The structural basis for the integrity of adenovirus Ad3 dodecahedron.Ewa SzolajskaWim P BurmeisterMonika ZochowskaBarbara NerloIgor AndreevGuy SchoehnJean-Pierre AndrieuPascal FenderAntonina NaskalskaChloe ZubietaStephen CusackJadwiga ChroboczekDuring the viral life cycle adenoviruses produce excess capsid proteins. Human adenovirus serotype 3 (Ad3) synthesizes predominantly an excess of free pentons, the complexes of pentameric penton base and trimeric fiber proteins, which are responsible for virus penetration. In infected cells Ad3 pentons spontaneously assemble into dodecahedral virus-like nano-particles containing twelve pentons. They also form in insect cells during expression in the baculovirus system. Similarly, in the absence of fiber protein dodecahedric particles built of 12 penton base pentamers can be produced. Both kinds of dodecahedra show remarkable efficiency of intracellular penetration and can be engineered to deliver several millions of foreign cargo molecules to a single target cell. For this reason, they are of great interest as a delivery vector. In order to successfully manipulate this potential vector for drug and/or gene delivery, an understanding of the molecular basis of vector assembly and integrity is critical. Crystallographic data in conjunction with site-directed mutagenesis and biochemical analysis provide a model for the molecular determinants of dodecamer particle assembly and the requirements for stability. The 3.8 Å crystal structure of Ad3 penton base dodecamer (Dd) shows that the dodecahedric structure is stabilized by strand-swapping between neighboring penton base molecules. Such N-terminal strand-swapping does not occur for Dd of Ad2, a serotype which does not form Dd under physiological conditions. This unique stabilization of the Ad3 dodecamer is controlled by residues 59-61 located at the site of strand switching, the residues involved in putative salt bridges between pentamers and by the disordered N-terminus (residues 1-47), as confirmed by site directed mutagenesis and biochemical analysis of mutant and wild type protein. We also provide evidence that the distal N-terminal residues are externally exposed and available for attaching cargo.http://europepmc.org/articles/PMC3457955?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ewa Szolajska Wim P Burmeister Monika Zochowska Barbara Nerlo Igor Andreev Guy Schoehn Jean-Pierre Andrieu Pascal Fender Antonina Naskalska Chloe Zubieta Stephen Cusack Jadwiga Chroboczek |
spellingShingle |
Ewa Szolajska Wim P Burmeister Monika Zochowska Barbara Nerlo Igor Andreev Guy Schoehn Jean-Pierre Andrieu Pascal Fender Antonina Naskalska Chloe Zubieta Stephen Cusack Jadwiga Chroboczek The structural basis for the integrity of adenovirus Ad3 dodecahedron. PLoS ONE |
author_facet |
Ewa Szolajska Wim P Burmeister Monika Zochowska Barbara Nerlo Igor Andreev Guy Schoehn Jean-Pierre Andrieu Pascal Fender Antonina Naskalska Chloe Zubieta Stephen Cusack Jadwiga Chroboczek |
author_sort |
Ewa Szolajska |
title |
The structural basis for the integrity of adenovirus Ad3 dodecahedron. |
title_short |
The structural basis for the integrity of adenovirus Ad3 dodecahedron. |
title_full |
The structural basis for the integrity of adenovirus Ad3 dodecahedron. |
title_fullStr |
The structural basis for the integrity of adenovirus Ad3 dodecahedron. |
title_full_unstemmed |
The structural basis for the integrity of adenovirus Ad3 dodecahedron. |
title_sort |
structural basis for the integrity of adenovirus ad3 dodecahedron. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
During the viral life cycle adenoviruses produce excess capsid proteins. Human adenovirus serotype 3 (Ad3) synthesizes predominantly an excess of free pentons, the complexes of pentameric penton base and trimeric fiber proteins, which are responsible for virus penetration. In infected cells Ad3 pentons spontaneously assemble into dodecahedral virus-like nano-particles containing twelve pentons. They also form in insect cells during expression in the baculovirus system. Similarly, in the absence of fiber protein dodecahedric particles built of 12 penton base pentamers can be produced. Both kinds of dodecahedra show remarkable efficiency of intracellular penetration and can be engineered to deliver several millions of foreign cargo molecules to a single target cell. For this reason, they are of great interest as a delivery vector. In order to successfully manipulate this potential vector for drug and/or gene delivery, an understanding of the molecular basis of vector assembly and integrity is critical. Crystallographic data in conjunction with site-directed mutagenesis and biochemical analysis provide a model for the molecular determinants of dodecamer particle assembly and the requirements for stability. The 3.8 Å crystal structure of Ad3 penton base dodecamer (Dd) shows that the dodecahedric structure is stabilized by strand-swapping between neighboring penton base molecules. Such N-terminal strand-swapping does not occur for Dd of Ad2, a serotype which does not form Dd under physiological conditions. This unique stabilization of the Ad3 dodecamer is controlled by residues 59-61 located at the site of strand switching, the residues involved in putative salt bridges between pentamers and by the disordered N-terminus (residues 1-47), as confirmed by site directed mutagenesis and biochemical analysis of mutant and wild type protein. We also provide evidence that the distal N-terminal residues are externally exposed and available for attaching cargo. |
url |
http://europepmc.org/articles/PMC3457955?pdf=render |
work_keys_str_mv |
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