Construction of the hit-deficient mutant strain of Streptococcus mutans ATCC25175

Construction of the hit-deficient mutant strain of Streptococcus mutans ATCC25175

Objective To construct a hit-deficient mutant strain of S. mutans ATCC25175 and verify its cell cycle regulatory function. Method Genomic DNA was extracted from S. mutans ATCC25175 strains, and then the upstream and downstream DNA fragments of the hit gene were cloned into the pFW5 vector (spectinom...

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Bibliographic Details
Main Authors: LAI Yangfan, WANG Peng, QIAO Li, LIU Zhongjing, YE Zhaoyang, LIANG Yan
Format: Article
Language:zho
Published: Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases 2021-12-01
Series:口腔疾病防治
Subjects:
streptococcus mutans
hit gene
pfw5 vector
natural genetic transformation
homologous recombination
hit-deficient mutant strain
cell growth
cell-cycle regulation
Online Access:http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2021.12.002

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http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2021.12.002

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