Osmotic Stress Induced Cell Death in Wheat Is Alleviated by Tauroursodeoxycholic Acid and Involves Endoplasmic Reticulum Stress–Related Gene Expression

Although, tauroursodeoxycholic acid (TUDCA) has been widely studied in mammalian cells because of its role in inhibiting apoptosis, its effects on plants remain almost unknown, especially in the case of crops such as wheat. In this study, we conducted a series of experiments to explore the effects a...

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Main Authors: Liting Zhang, Zeyu Xin, Xing Yu, Chao Ma, Weiwei Liang, Meichen Zhu, Qiwei Cheng, Zongzhen Li, Yanan Niu, Yongzhe Ren, Zhiqiang Wang, Tongbao Lin
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-05-01
Series:Frontiers in Plant Science
Subjects:
ROS
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2017.00667/full
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record_format Article
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language English
format Article
sources DOAJ
author Liting Zhang
Liting Zhang
Liting Zhang
Zeyu Xin
Zeyu Xin
Zeyu Xin
Xing Yu
Xing Yu
Xing Yu
Chao Ma
Weiwei Liang
Weiwei Liang
Weiwei Liang
Meichen Zhu
Meichen Zhu
Meichen Zhu
Qiwei Cheng
Qiwei Cheng
Qiwei Cheng
Zongzhen Li
Zongzhen Li
Zongzhen Li
Yanan Niu
Yongzhe Ren
Yongzhe Ren
Yongzhe Ren
Zhiqiang Wang
Zhiqiang Wang
Zhiqiang Wang
Tongbao Lin
Tongbao Lin
Tongbao Lin
spellingShingle Liting Zhang
Liting Zhang
Liting Zhang
Zeyu Xin
Zeyu Xin
Zeyu Xin
Xing Yu
Xing Yu
Xing Yu
Chao Ma
Weiwei Liang
Weiwei Liang
Weiwei Liang
Meichen Zhu
Meichen Zhu
Meichen Zhu
Qiwei Cheng
Qiwei Cheng
Qiwei Cheng
Zongzhen Li
Zongzhen Li
Zongzhen Li
Yanan Niu
Yongzhe Ren
Yongzhe Ren
Yongzhe Ren
Zhiqiang Wang
Zhiqiang Wang
Zhiqiang Wang
Tongbao Lin
Tongbao Lin
Tongbao Lin
Osmotic Stress Induced Cell Death in Wheat Is Alleviated by Tauroursodeoxycholic Acid and Involves Endoplasmic Reticulum Stress–Related Gene Expression
Frontiers in Plant Science
osmotic stress
TUDCA
ER stress
ROS
cell death
author_facet Liting Zhang
Liting Zhang
Liting Zhang
Zeyu Xin
Zeyu Xin
Zeyu Xin
Xing Yu
Xing Yu
Xing Yu
Chao Ma
Weiwei Liang
Weiwei Liang
Weiwei Liang
Meichen Zhu
Meichen Zhu
Meichen Zhu
Qiwei Cheng
Qiwei Cheng
Qiwei Cheng
Zongzhen Li
Zongzhen Li
Zongzhen Li
Yanan Niu
Yongzhe Ren
Yongzhe Ren
Yongzhe Ren
Zhiqiang Wang
Zhiqiang Wang
Zhiqiang Wang
Tongbao Lin
Tongbao Lin
Tongbao Lin
author_sort Liting Zhang
title Osmotic Stress Induced Cell Death in Wheat Is Alleviated by Tauroursodeoxycholic Acid and Involves Endoplasmic Reticulum Stress–Related Gene Expression
title_short Osmotic Stress Induced Cell Death in Wheat Is Alleviated by Tauroursodeoxycholic Acid and Involves Endoplasmic Reticulum Stress–Related Gene Expression
title_full Osmotic Stress Induced Cell Death in Wheat Is Alleviated by Tauroursodeoxycholic Acid and Involves Endoplasmic Reticulum Stress–Related Gene Expression
title_fullStr Osmotic Stress Induced Cell Death in Wheat Is Alleviated by Tauroursodeoxycholic Acid and Involves Endoplasmic Reticulum Stress–Related Gene Expression
title_full_unstemmed Osmotic Stress Induced Cell Death in Wheat Is Alleviated by Tauroursodeoxycholic Acid and Involves Endoplasmic Reticulum Stress–Related Gene Expression
title_sort osmotic stress induced cell death in wheat is alleviated by tauroursodeoxycholic acid and involves endoplasmic reticulum stress–related gene expression
publisher Frontiers Media S.A.
series Frontiers in Plant Science
issn 1664-462X
publishDate 2017-05-01
description Although, tauroursodeoxycholic acid (TUDCA) has been widely studied in mammalian cells because of its role in inhibiting apoptosis, its effects on plants remain almost unknown, especially in the case of crops such as wheat. In this study, we conducted a series of experiments to explore the effects and mechanisms of action of TUDCA on wheat growth and cell death induced by osmotic stress. Our results show that TUDCA: (1) ameliorates the impact of osmotic stress on wheat height, fresh weight, and water content; (2) alleviates the decrease in chlorophyll content as well as membrane damage caused by osmotic stress; (3) decreases the accumulation of reactive oxygen species (ROS) by increasing the activity of antioxidant enzymes under osmotic stress; and (4) to some extent alleviates osmotic stress–induced cell death probably by regulating endoplasmic reticulum (ER) stress–related gene expression, for example expression of the basic leucine zipper genes bZIP60B and bZIP60D, the binding proteins BiP1 and BiP2, the protein disulfide isomerase PDIL8-1, and the glucose-regulated protein GRP94. We also propose a model that illustrates how TUDCA alleviates osmotic stress–related wheat cell death, which provides an important theoretical basis for improving plant stress adaptation and elucidates the mechanisms of ER stress–related plant osmotic stress resistance.
topic osmotic stress
TUDCA
ER stress
ROS
cell death
url http://journal.frontiersin.org/article/10.3389/fpls.2017.00667/full
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spelling doaj-3a93cc4670624a519c3ef5e2f2c1eeb52020-11-25T01:10:21ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-05-01810.3389/fpls.2017.00667241091Osmotic Stress Induced Cell Death in Wheat Is Alleviated by Tauroursodeoxycholic Acid and Involves Endoplasmic Reticulum Stress–Related Gene ExpressionLiting Zhang0Liting Zhang1Liting Zhang2Zeyu Xin3Zeyu Xin4Zeyu Xin5Xing Yu6Xing Yu7Xing Yu8Chao Ma9Weiwei Liang10Weiwei Liang11Weiwei Liang12Meichen Zhu13Meichen Zhu14Meichen Zhu15Qiwei Cheng16Qiwei Cheng17Qiwei Cheng18Zongzhen Li19Zongzhen Li20Zongzhen Li21Yanan Niu22Yongzhe Ren23Yongzhe Ren24Yongzhe Ren25Zhiqiang Wang26Zhiqiang Wang27Zhiqiang Wang28Tongbao Lin29Tongbao Lin30Tongbao Lin31College of Agronomy, Henan Agricultural UniversityZhengzhou, ChinaCollaborative Innovation Center of Henan Grain CropsZhengzhou, ChinaNational Key Laboratory of Wheat and Maize Crop ScienceZhengzhou, ChinaCollege of Agronomy, Henan Agricultural UniversityZhengzhou, ChinaCollaborative Innovation Center of Henan Grain CropsZhengzhou, ChinaNational Key Laboratory of Wheat and Maize Crop ScienceZhengzhou, ChinaCollege of Agronomy, Henan Agricultural UniversityZhengzhou, ChinaCollaborative Innovation Center of Henan Grain CropsZhengzhou, ChinaNational Key Laboratory of Wheat and Maize Crop ScienceZhengzhou, ChinaCollege of Agronomy, Henan University of Science and TechnologyLuoyang, ChinaCollege of Agronomy, Henan Agricultural UniversityZhengzhou, ChinaCollaborative Innovation Center of Henan Grain CropsZhengzhou, ChinaNational Key Laboratory of Wheat and Maize Crop ScienceZhengzhou, ChinaCollege of Agronomy, Henan Agricultural UniversityZhengzhou, ChinaCollaborative Innovation Center of Henan Grain CropsZhengzhou, ChinaNational Key Laboratory of Wheat and Maize Crop ScienceZhengzhou, ChinaCollege of Agronomy, Henan Agricultural UniversityZhengzhou, ChinaCollaborative Innovation Center of Henan Grain CropsZhengzhou, ChinaNational Key Laboratory of Wheat and Maize Crop ScienceZhengzhou, ChinaCollege of Agronomy, Henan Agricultural UniversityZhengzhou, ChinaCollaborative Innovation Center of Henan Grain CropsZhengzhou, ChinaNational Key Laboratory of Wheat and Maize Crop ScienceZhengzhou, ChinaCollege of Agronomy, Henan Agricultural UniversityZhengzhou, ChinaCollege of Agronomy, Henan Agricultural UniversityZhengzhou, ChinaCollaborative Innovation Center of Henan Grain CropsZhengzhou, ChinaNational Key Laboratory of Wheat and Maize Crop ScienceZhengzhou, ChinaCollege of Agronomy, Henan Agricultural UniversityZhengzhou, ChinaCollaborative Innovation Center of Henan Grain CropsZhengzhou, ChinaNational Key Laboratory of Wheat and Maize Crop ScienceZhengzhou, ChinaCollege of Agronomy, Henan Agricultural UniversityZhengzhou, ChinaCollaborative Innovation Center of Henan Grain CropsZhengzhou, ChinaNational Key Laboratory of Wheat and Maize Crop ScienceZhengzhou, ChinaAlthough, tauroursodeoxycholic acid (TUDCA) has been widely studied in mammalian cells because of its role in inhibiting apoptosis, its effects on plants remain almost unknown, especially in the case of crops such as wheat. In this study, we conducted a series of experiments to explore the effects and mechanisms of action of TUDCA on wheat growth and cell death induced by osmotic stress. Our results show that TUDCA: (1) ameliorates the impact of osmotic stress on wheat height, fresh weight, and water content; (2) alleviates the decrease in chlorophyll content as well as membrane damage caused by osmotic stress; (3) decreases the accumulation of reactive oxygen species (ROS) by increasing the activity of antioxidant enzymes under osmotic stress; and (4) to some extent alleviates osmotic stress–induced cell death probably by regulating endoplasmic reticulum (ER) stress–related gene expression, for example expression of the basic leucine zipper genes bZIP60B and bZIP60D, the binding proteins BiP1 and BiP2, the protein disulfide isomerase PDIL8-1, and the glucose-regulated protein GRP94. We also propose a model that illustrates how TUDCA alleviates osmotic stress–related wheat cell death, which provides an important theoretical basis for improving plant stress adaptation and elucidates the mechanisms of ER stress–related plant osmotic stress resistance.http://journal.frontiersin.org/article/10.3389/fpls.2017.00667/fullosmotic stressTUDCAER stressROScell death