Chemical Analysis of Lipid Boundaries after Consecutive Growth and Division of Supported Giant Vesicles
Summary: The reproduction of the shape of giant vesicles usually results in the increase of their “population” size. This may be achieved on giant vesicles by appropriately supplying “mother” vesicles with membranogenic amphiphiles. The next “generation” of “daughter” vesicles obtained from this “fe...
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doaj-3b1db8a3099341b7aa5b9f382613d6d22020-11-25T03:10:20ZengElsevieriScience2589-00422020-11-012311101677Chemical Analysis of Lipid Boundaries after Consecutive Growth and Division of Supported Giant VesiclesAugustin Lopez0Dimitri Fayolle1Michele Fiore2Peter Strazewski3Université de Lyon, Université Claude Bernard Lyon 1, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Bâtiment Edgar Lederer, 1 Rue Victor Grignard, 69622 Villeurbanne Cedex, FranceUniversité de Lyon, Université Claude Bernard Lyon 1, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Bâtiment Edgar Lederer, 1 Rue Victor Grignard, 69622 Villeurbanne Cedex, FranceUniversité de Lyon, Université Claude Bernard Lyon 1, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Bâtiment Edgar Lederer, 1 Rue Victor Grignard, 69622 Villeurbanne Cedex, France; Corresponding authorUniversité de Lyon, Université Claude Bernard Lyon 1, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Bâtiment Edgar Lederer, 1 Rue Victor Grignard, 69622 Villeurbanne Cedex, FranceSummary: The reproduction of the shape of giant vesicles usually results in the increase of their “population” size. This may be achieved on giant vesicles by appropriately supplying “mother” vesicles with membranogenic amphiphiles. The next “generation” of “daughter” vesicles obtained from this “feeding” is inherently difficult to distinguish from the original mothers. Here we report on a method for the consecutive feeding with different fatty acids that each provoke membrane growth and detachment of daughter vesicles from glass microsphere-supported phospholipidic mother vesicles. We discovered that a saturated fatty acid was carried over to the next generation of mothers better than two unsaturated congeners. This has an important bearing on the growth and replication of primitive compartments at the early stages of life. Microsphere-supported vesicles are also a precise analytical tool.http://www.sciencedirect.com/science/article/pii/S2589004220308695Soft MatterMaterials ScienceColloids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Augustin Lopez Dimitri Fayolle Michele Fiore Peter Strazewski |
spellingShingle |
Augustin Lopez Dimitri Fayolle Michele Fiore Peter Strazewski Chemical Analysis of Lipid Boundaries after Consecutive Growth and Division of Supported Giant Vesicles iScience Soft Matter Materials Science Colloids |
author_facet |
Augustin Lopez Dimitri Fayolle Michele Fiore Peter Strazewski |
author_sort |
Augustin Lopez |
title |
Chemical Analysis of Lipid Boundaries after Consecutive Growth and Division of Supported Giant Vesicles |
title_short |
Chemical Analysis of Lipid Boundaries after Consecutive Growth and Division of Supported Giant Vesicles |
title_full |
Chemical Analysis of Lipid Boundaries after Consecutive Growth and Division of Supported Giant Vesicles |
title_fullStr |
Chemical Analysis of Lipid Boundaries after Consecutive Growth and Division of Supported Giant Vesicles |
title_full_unstemmed |
Chemical Analysis of Lipid Boundaries after Consecutive Growth and Division of Supported Giant Vesicles |
title_sort |
chemical analysis of lipid boundaries after consecutive growth and division of supported giant vesicles |
publisher |
Elsevier |
series |
iScience |
issn |
2589-0042 |
publishDate |
2020-11-01 |
description |
Summary: The reproduction of the shape of giant vesicles usually results in the increase of their “population” size. This may be achieved on giant vesicles by appropriately supplying “mother” vesicles with membranogenic amphiphiles. The next “generation” of “daughter” vesicles obtained from this “feeding” is inherently difficult to distinguish from the original mothers. Here we report on a method for the consecutive feeding with different fatty acids that each provoke membrane growth and detachment of daughter vesicles from glass microsphere-supported phospholipidic mother vesicles. We discovered that a saturated fatty acid was carried over to the next generation of mothers better than two unsaturated congeners. This has an important bearing on the growth and replication of primitive compartments at the early stages of life. Microsphere-supported vesicles are also a precise analytical tool. |
topic |
Soft Matter Materials Science Colloids |
url |
http://www.sciencedirect.com/science/article/pii/S2589004220308695 |
work_keys_str_mv |
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