Association of IL-1β, IL-6, TNF-α, and TGFβ1 Gene Polymorphisms with Recurrent Spontaneous Abortion in Polycystic Ovary Syndrome

Recurrent spontaneous abortion (RSA) is a common pregnancy-associated complication of polycystic ovary syndrome (PCOS) which is an endocrine malfunction disease. Patients with PCOS may have several underlying contributing and interrelated factors, which have been reported in women with RSA. The inci...

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Main Authors: Afrah F. Alkhuriji, Suliman Y. Al Omar, Zainb A. Babay, Manal F. El-khadragy, Lamjed A. Mansour, Wazirah G. Alharbi, Mahmoud I. Khalil
Format: Article
Language:English
Published: Hindawi Limited 2020-01-01
Series:Disease Markers
Online Access:http://dx.doi.org/10.1155/2020/6076274
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spelling doaj-3b44d3bd03394d5894cadb0e3dfe69a92020-11-25T02:05:58ZengHindawi LimitedDisease Markers0278-02401875-86302020-01-01202010.1155/2020/60762746076274Association of IL-1β, IL-6, TNF-α, and TGFβ1 Gene Polymorphisms with Recurrent Spontaneous Abortion in Polycystic Ovary SyndromeAfrah F. Alkhuriji0Suliman Y. Al Omar1Zainb A. Babay2Manal F. El-khadragy3Lamjed A. Mansour4Wazirah G. Alharbi5Mahmoud I. Khalil6Department of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Obstetrics and Gynaecology, King Saud University, King Khalid University Hospital, Riyadh, Saudi ArabiaDepartment of Biology, Faculty of Science, Princess Nourah Bint Abdulrahman University, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Biological Sciences, Faculty of Science, Beirut Arab University, LebanonRecurrent spontaneous abortion (RSA) is a common pregnancy-associated complication of polycystic ovary syndrome (PCOS) which is an endocrine malfunction disease. Patients with PCOS may have several underlying contributing and interrelated factors, which have been reported in women with RSA. The incidence rate between PCOS and RSA remains uncertain. The aim of this study is to determine the possible association of IL-1β-511C/T, IL-6-174G/C, TNF-α-1031T/C, and TGFβ1-509T/C with RSA patients with or without PCOS. A total of 140 RSA patients, 70 of which were PCOS patients, and 140 healthy females with no history of RSA or PCOS were included in this study. PCR amplification, genotyping, and sequence analysis were employed to investigate the presence of the polymorphisms. The genotypic and allelic frequencies were calculated separately for each subject. Out of the four studied polymorphisms, the IL-1β-511C/T genotype in RSA without PCOS patients (12.7%) was significantly different compared with that in control subjects (p=0.047). For IL-6-174C/G, there was a tendency towards more CC carriers among RSA with PCOS patients (10%) than in controls (3%). The GG genotype in RSA women with PCOS (60%) was significantly different compared with that in control subjects (p=0.033), and the GC genotype in RSA with PCOS patients (30%) showed a marginal significant difference compared with that in control subjects (p=0.050). Significant difference was identified in the allelic frequencies in RSA patients with PCOS compared to controls (p=0.025). IL-6-174G/C and TNF-α-1031T/C polymorphisms are significantly associated with RSA patients in Saudi patients with PCOS, while the IL-1β-511C/T polymorphism is significantly associated with RSA patients without PCOS.http://dx.doi.org/10.1155/2020/6076274
collection DOAJ
language English
format Article
sources DOAJ
author Afrah F. Alkhuriji
Suliman Y. Al Omar
Zainb A. Babay
Manal F. El-khadragy
Lamjed A. Mansour
Wazirah G. Alharbi
Mahmoud I. Khalil
spellingShingle Afrah F. Alkhuriji
Suliman Y. Al Omar
Zainb A. Babay
Manal F. El-khadragy
Lamjed A. Mansour
Wazirah G. Alharbi
Mahmoud I. Khalil
Association of IL-1β, IL-6, TNF-α, and TGFβ1 Gene Polymorphisms with Recurrent Spontaneous Abortion in Polycystic Ovary Syndrome
Disease Markers
author_facet Afrah F. Alkhuriji
Suliman Y. Al Omar
Zainb A. Babay
Manal F. El-khadragy
Lamjed A. Mansour
Wazirah G. Alharbi
Mahmoud I. Khalil
author_sort Afrah F. Alkhuriji
title Association of IL-1β, IL-6, TNF-α, and TGFβ1 Gene Polymorphisms with Recurrent Spontaneous Abortion in Polycystic Ovary Syndrome
title_short Association of IL-1β, IL-6, TNF-α, and TGFβ1 Gene Polymorphisms with Recurrent Spontaneous Abortion in Polycystic Ovary Syndrome
title_full Association of IL-1β, IL-6, TNF-α, and TGFβ1 Gene Polymorphisms with Recurrent Spontaneous Abortion in Polycystic Ovary Syndrome
title_fullStr Association of IL-1β, IL-6, TNF-α, and TGFβ1 Gene Polymorphisms with Recurrent Spontaneous Abortion in Polycystic Ovary Syndrome
title_full_unstemmed Association of IL-1β, IL-6, TNF-α, and TGFβ1 Gene Polymorphisms with Recurrent Spontaneous Abortion in Polycystic Ovary Syndrome
title_sort association of il-1β, il-6, tnf-α, and tgfβ1 gene polymorphisms with recurrent spontaneous abortion in polycystic ovary syndrome
publisher Hindawi Limited
series Disease Markers
issn 0278-0240
1875-8630
publishDate 2020-01-01
description Recurrent spontaneous abortion (RSA) is a common pregnancy-associated complication of polycystic ovary syndrome (PCOS) which is an endocrine malfunction disease. Patients with PCOS may have several underlying contributing and interrelated factors, which have been reported in women with RSA. The incidence rate between PCOS and RSA remains uncertain. The aim of this study is to determine the possible association of IL-1β-511C/T, IL-6-174G/C, TNF-α-1031T/C, and TGFβ1-509T/C with RSA patients with or without PCOS. A total of 140 RSA patients, 70 of which were PCOS patients, and 140 healthy females with no history of RSA or PCOS were included in this study. PCR amplification, genotyping, and sequence analysis were employed to investigate the presence of the polymorphisms. The genotypic and allelic frequencies were calculated separately for each subject. Out of the four studied polymorphisms, the IL-1β-511C/T genotype in RSA without PCOS patients (12.7%) was significantly different compared with that in control subjects (p=0.047). For IL-6-174C/G, there was a tendency towards more CC carriers among RSA with PCOS patients (10%) than in controls (3%). The GG genotype in RSA women with PCOS (60%) was significantly different compared with that in control subjects (p=0.033), and the GC genotype in RSA with PCOS patients (30%) showed a marginal significant difference compared with that in control subjects (p=0.050). Significant difference was identified in the allelic frequencies in RSA patients with PCOS compared to controls (p=0.025). IL-6-174G/C and TNF-α-1031T/C polymorphisms are significantly associated with RSA patients in Saudi patients with PCOS, while the IL-1β-511C/T polymorphism is significantly associated with RSA patients without PCOS.
url http://dx.doi.org/10.1155/2020/6076274
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