Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion

The host-guest complexation between an Alexa 488 labelled adamantane derivative and β-cyclodextrin is studied by Fluorescence Correlation Spectroscopy (FCS). A 1:1 complex stoichiometry and a high association equilibrium constant of K = 5.2 × 104 M-1 are obtained in aqueous solution at 25 °C and pH...

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Main Authors: Wajih Al-Soufi, Mercedes Novo, Maria Jesus Pérez-Alvite, Jorge Bordello, Daniel Granadero
Format: Article
Language:English
Published: MDPI AG 2010-01-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:http://www.mdpi.com/1422-0067/11/1/173/
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spelling doaj-3c47e50375e347b6b852975e8a077d392020-11-25T00:52:16ZengMDPI AGInternational Journal of Molecular Sciences1422-00672010-01-0111117318810.3390/ijms11010173Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin InclusionWajih Al-SoufiMercedes NovoMaria Jesus Pérez-AlviteJorge BordelloDaniel GranaderoThe host-guest complexation between an Alexa 488 labelled adamantane derivative and β-cyclodextrin is studied by Fluorescence Correlation Spectroscopy (FCS). A 1:1 complex stoichiometry and a high association equilibrium constant of K = 5.2 × 104 M-1 are obtained in aqueous solution at 25 °C and pH = 6. The necessary experimental conditions are discussed. FCS proves to be an excellent method for the determination of stoichiometry and association equilibrium constant of this type of complexes, where both host and guest are nonfluorescent and which are therefore not easily amenable to standard fluorescence spectroscopic methods. http://www.mdpi.com/1422-0067/11/1/173/fluorescence correlation spectroscopyhost-guest chemistryadamantanecyclodextrin
collection DOAJ
language English
format Article
sources DOAJ
author Wajih Al-Soufi
Mercedes Novo
Maria Jesus Pérez-Alvite
Jorge Bordello
Daniel Granadero
spellingShingle Wajih Al-Soufi
Mercedes Novo
Maria Jesus Pérez-Alvite
Jorge Bordello
Daniel Granadero
Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion
International Journal of Molecular Sciences
fluorescence correlation spectroscopy
host-guest chemistry
adamantane
cyclodextrin
author_facet Wajih Al-Soufi
Mercedes Novo
Maria Jesus Pérez-Alvite
Jorge Bordello
Daniel Granadero
author_sort Wajih Al-Soufi
title Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion
title_short Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion
title_full Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion
title_fullStr Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion
title_full_unstemmed Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion
title_sort host-guest complexation studied by fluorescence correlation spectroscopy: adamantane–cyclodextrin inclusion
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2010-01-01
description The host-guest complexation between an Alexa 488 labelled adamantane derivative and β-cyclodextrin is studied by Fluorescence Correlation Spectroscopy (FCS). A 1:1 complex stoichiometry and a high association equilibrium constant of K = 5.2 × 104 M-1 are obtained in aqueous solution at 25 °C and pH = 6. The necessary experimental conditions are discussed. FCS proves to be an excellent method for the determination of stoichiometry and association equilibrium constant of this type of complexes, where both host and guest are nonfluorescent and which are therefore not easily amenable to standard fluorescence spectroscopic methods.
topic fluorescence correlation spectroscopy
host-guest chemistry
adamantane
cyclodextrin
url http://www.mdpi.com/1422-0067/11/1/173/
work_keys_str_mv AT wajihalsoufi hostguestcomplexationstudiedbyfluorescencecorrelationspectroscopyadamantanecyclodextrininclusion
AT mercedesnovo hostguestcomplexationstudiedbyfluorescencecorrelationspectroscopyadamantanecyclodextrininclusion
AT mariajesusperezalvite hostguestcomplexationstudiedbyfluorescencecorrelationspectroscopyadamantanecyclodextrininclusion
AT jorgebordello hostguestcomplexationstudiedbyfluorescencecorrelationspectroscopyadamantanecyclodextrininclusion
AT danielgranadero hostguestcomplexationstudiedbyfluorescencecorrelationspectroscopyadamantanecyclodextrininclusion
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