Cloning, molecular and functional characterization by overexpression in Arabidopsis of MAPKK genes from grapevine (Vitis vinifera)
Abstract Background The mitogen-activated protein kinases (MAPKs), as a part of the MAPKKK-MAPKK-MAPK cascade, play crucial roles in plant development as an intracellular signal transduction pathway to respond various environmental signals. However, few MAPKK have been functionally characterized in...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2020-05-01
|
Series: | BMC Plant Biology |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12870-020-02378-4 |
id |
doaj-3cbd9611c2e5487984b1d6d5eac25a30 |
---|---|
record_format |
Article |
spelling |
doaj-3cbd9611c2e5487984b1d6d5eac25a302020-11-25T03:10:25ZengBMCBMC Plant Biology1471-22292020-05-0120111510.1186/s12870-020-02378-4Cloning, molecular and functional characterization by overexpression in Arabidopsis of MAPKK genes from grapevine (Vitis vinifera)Gang Wang0Ying-hai Liang1Ji-yu Zhang2Zong-Ming ( Max) Cheng3College of Horticulture, Nanjing Agricultural UniversityInstitute of Pomology, Jilin Academy of Agricultural SciencesInstitute of Botany, Jiangsu Province and Chinese Academy of SciencesCollege of Horticulture, Nanjing Agricultural UniversityAbstract Background The mitogen-activated protein kinases (MAPKs), as a part of the MAPKKK-MAPKK-MAPK cascade, play crucial roles in plant development as an intracellular signal transduction pathway to respond various environmental signals. However, few MAPKK have been functionally characterized in grapevine. Results In the study, five MAPKK (MKK) members were identified in grapevine (cultivar ‘Pinot Noir’), cloned and designated as VvMKK1-VvMKK5. A phylogenetic analysis grouped them into four sub-families based on the similarity of their conserved motifs and gene structure to Arabidopsis MAPKK members. qRT-PCR results indicated that the expression of VvMKK1, VvMKK2, VvMKK4, and VvMKK5 were up-regulated in mature leaf and young blades, and roots, but exhibited low expression in leaf petioles. VvMKK2, VvMKK3, and VvMKK5 genes were differentially up-regulated when grapevine leaves were inoculated with spores of Erisyphe necator, or treated with salicylic acid (SA), ethylene (ETH), H2O2, or exposed to drought, indicating that these genes may be involved in a variety of signaling pathways. Over expression of VvMKK2 and VvMKK4 genes in transgenic Arabidopsis plants resulted in the production of seeds with a significantly higher germination and survival rate, and better seedling growth under stress conditions than wild-type plants. Overexpression of VvMKK2 in Arabidopsis improved salt and drought stress tolerance while overexpression of VvMKK4 only improved salt stress tolerance. Conclusions Results of the present investigation provide a better understanding of the interaction and function of MAPKKK-MAPKK-MAPK genes at the transcriptional level in grapevine and led to the identification of candidate genes for drought and salt stress in grapes.http://link.springer.com/article/10.1186/s12870-020-02378-4GrapevineMitogen-activated protein kinase kinase (MAPKK/MKK)Expression analysisSalt and drought stressesTransgenic plants |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gang Wang Ying-hai Liang Ji-yu Zhang Zong-Ming ( Max) Cheng |
spellingShingle |
Gang Wang Ying-hai Liang Ji-yu Zhang Zong-Ming ( Max) Cheng Cloning, molecular and functional characterization by overexpression in Arabidopsis of MAPKK genes from grapevine (Vitis vinifera) BMC Plant Biology Grapevine Mitogen-activated protein kinase kinase (MAPKK/MKK) Expression analysis Salt and drought stresses Transgenic plants |
author_facet |
Gang Wang Ying-hai Liang Ji-yu Zhang Zong-Ming ( Max) Cheng |
author_sort |
Gang Wang |
title |
Cloning, molecular and functional characterization by overexpression in Arabidopsis of MAPKK genes from grapevine (Vitis vinifera) |
title_short |
Cloning, molecular and functional characterization by overexpression in Arabidopsis of MAPKK genes from grapevine (Vitis vinifera) |
title_full |
Cloning, molecular and functional characterization by overexpression in Arabidopsis of MAPKK genes from grapevine (Vitis vinifera) |
title_fullStr |
Cloning, molecular and functional characterization by overexpression in Arabidopsis of MAPKK genes from grapevine (Vitis vinifera) |
title_full_unstemmed |
Cloning, molecular and functional characterization by overexpression in Arabidopsis of MAPKK genes from grapevine (Vitis vinifera) |
title_sort |
cloning, molecular and functional characterization by overexpression in arabidopsis of mapkk genes from grapevine (vitis vinifera) |
publisher |
BMC |
series |
BMC Plant Biology |
issn |
1471-2229 |
publishDate |
2020-05-01 |
description |
Abstract Background The mitogen-activated protein kinases (MAPKs), as a part of the MAPKKK-MAPKK-MAPK cascade, play crucial roles in plant development as an intracellular signal transduction pathway to respond various environmental signals. However, few MAPKK have been functionally characterized in grapevine. Results In the study, five MAPKK (MKK) members were identified in grapevine (cultivar ‘Pinot Noir’), cloned and designated as VvMKK1-VvMKK5. A phylogenetic analysis grouped them into four sub-families based on the similarity of their conserved motifs and gene structure to Arabidopsis MAPKK members. qRT-PCR results indicated that the expression of VvMKK1, VvMKK2, VvMKK4, and VvMKK5 were up-regulated in mature leaf and young blades, and roots, but exhibited low expression in leaf petioles. VvMKK2, VvMKK3, and VvMKK5 genes were differentially up-regulated when grapevine leaves were inoculated with spores of Erisyphe necator, or treated with salicylic acid (SA), ethylene (ETH), H2O2, or exposed to drought, indicating that these genes may be involved in a variety of signaling pathways. Over expression of VvMKK2 and VvMKK4 genes in transgenic Arabidopsis plants resulted in the production of seeds with a significantly higher germination and survival rate, and better seedling growth under stress conditions than wild-type plants. Overexpression of VvMKK2 in Arabidopsis improved salt and drought stress tolerance while overexpression of VvMKK4 only improved salt stress tolerance. Conclusions Results of the present investigation provide a better understanding of the interaction and function of MAPKKK-MAPKK-MAPK genes at the transcriptional level in grapevine and led to the identification of candidate genes for drought and salt stress in grapes. |
topic |
Grapevine Mitogen-activated protein kinase kinase (MAPKK/MKK) Expression analysis Salt and drought stresses Transgenic plants |
url |
http://link.springer.com/article/10.1186/s12870-020-02378-4 |
work_keys_str_mv |
AT gangwang cloningmolecularandfunctionalcharacterizationbyoverexpressioninarabidopsisofmapkkgenesfromgrapevinevitisvinifera AT yinghailiang cloningmolecularandfunctionalcharacterizationbyoverexpressioninarabidopsisofmapkkgenesfromgrapevinevitisvinifera AT jiyuzhang cloningmolecularandfunctionalcharacterizationbyoverexpressioninarabidopsisofmapkkgenesfromgrapevinevitisvinifera AT zongmingmaxcheng cloningmolecularandfunctionalcharacterizationbyoverexpressioninarabidopsisofmapkkgenesfromgrapevinevitisvinifera |
_version_ |
1724658719686393856 |