The Acinetobacter baumannii Mla system and glycerophospholipid transport to the outer membrane
The outer membrane (OM) of Gram-negative bacteria serves as a selective permeability barrier that allows entry of essential nutrients while excluding toxic compounds, including antibiotics. The OM is asymmetric and contains an outer leaflet of lipopolysaccharides (LPS) or lipooligosaccharides (LOS)...
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doaj-3e878368b67745b59962862f6cdc5c942021-05-05T17:18:46ZengeLife Sciences Publications LtdeLife2050-084X2019-01-01810.7554/eLife.40171The Acinetobacter baumannii Mla system and glycerophospholipid transport to the outer membraneCassandra Kamischke0Junping Fan1Julien Bergeron2Hemantha D Kulasekara3Zachary D Dalebroux4Anika Burrell5Justin M Kollman6Samuel I Miller7https://orcid.org/0000-0003-1638-2181Department of Microbiology, University of Washington, Seattle, United StatesDepartment of Microbiology, University of Washington, Seattle, United StatesDepartment of Biochemistry, University of Washington, Seattle, United States; Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield, United KingdomDepartment of Microbiology, University of Washington, Seattle, United StatesDepartment of Microbiology, University of Washington, Seattle, United StatesDepartment of Biochemistry, University of Washington, Seattle, United StatesDepartment of Biochemistry, University of Washington, Seattle, United StatesDepartment of Microbiology, University of Washington, Seattle, United States; Department of Genome Sciences, University of Washington, Seattle, United States; Department of Medicine, University of Washington, Seattle, United StatesThe outer membrane (OM) of Gram-negative bacteria serves as a selective permeability barrier that allows entry of essential nutrients while excluding toxic compounds, including antibiotics. The OM is asymmetric and contains an outer leaflet of lipopolysaccharides (LPS) or lipooligosaccharides (LOS) and an inner leaflet of glycerophospholipids (GPL). We screened Acinetobacter baumannii transposon mutants and identified a number of mutants with OM defects, including an ABC transporter system homologous to the Mla system in E. coli. We further show that this opportunistic, antibiotic-resistant pathogen uses this multicomponent protein complex and ATP hydrolysis at the inner membrane to promote GPL export to the OM. The broad conservation of the Mla system in Gram-negative bacteria suggests the system may play a conserved role in OM biogenesis. The importance of the Mla system to Acinetobacter baumannii OM integrity and antibiotic sensitivity suggests that its components may serve as new antimicrobial therapeutic targets.https://elifesciences.org/articles/40171Acinetobacter baumanniiglycerophospholipidsABC Transporter |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cassandra Kamischke Junping Fan Julien Bergeron Hemantha D Kulasekara Zachary D Dalebroux Anika Burrell Justin M Kollman Samuel I Miller |
spellingShingle |
Cassandra Kamischke Junping Fan Julien Bergeron Hemantha D Kulasekara Zachary D Dalebroux Anika Burrell Justin M Kollman Samuel I Miller The Acinetobacter baumannii Mla system and glycerophospholipid transport to the outer membrane eLife Acinetobacter baumannii glycerophospholipids ABC Transporter |
author_facet |
Cassandra Kamischke Junping Fan Julien Bergeron Hemantha D Kulasekara Zachary D Dalebroux Anika Burrell Justin M Kollman Samuel I Miller |
author_sort |
Cassandra Kamischke |
title |
The Acinetobacter baumannii Mla system and glycerophospholipid transport to the outer membrane |
title_short |
The Acinetobacter baumannii Mla system and glycerophospholipid transport to the outer membrane |
title_full |
The Acinetobacter baumannii Mla system and glycerophospholipid transport to the outer membrane |
title_fullStr |
The Acinetobacter baumannii Mla system and glycerophospholipid transport to the outer membrane |
title_full_unstemmed |
The Acinetobacter baumannii Mla system and glycerophospholipid transport to the outer membrane |
title_sort |
acinetobacter baumannii mla system and glycerophospholipid transport to the outer membrane |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2019-01-01 |
description |
The outer membrane (OM) of Gram-negative bacteria serves as a selective permeability barrier that allows entry of essential nutrients while excluding toxic compounds, including antibiotics. The OM is asymmetric and contains an outer leaflet of lipopolysaccharides (LPS) or lipooligosaccharides (LOS) and an inner leaflet of glycerophospholipids (GPL). We screened Acinetobacter baumannii transposon mutants and identified a number of mutants with OM defects, including an ABC transporter system homologous to the Mla system in E. coli. We further show that this opportunistic, antibiotic-resistant pathogen uses this multicomponent protein complex and ATP hydrolysis at the inner membrane to promote GPL export to the OM. The broad conservation of the Mla system in Gram-negative bacteria suggests the system may play a conserved role in OM biogenesis. The importance of the Mla system to Acinetobacter baumannii OM integrity and antibiotic sensitivity suggests that its components may serve as new antimicrobial therapeutic targets. |
topic |
Acinetobacter baumannii glycerophospholipids ABC Transporter |
url |
https://elifesciences.org/articles/40171 |
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