Ion Chromatographic Method with Post-Column Fuchsin Reaction for Measurement of Bromate in Chlorinated Water
An ion chromatographic method that employs a post-column reaction with fuchsin and spectrophotometric detection was optimized for measuring bromate (BrO3-) in water. BrO3- is converted to Br2 by sodium metabisulfite and then reacted with acidic fuchsin to form a red-colored product that strongly ab...
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University of the Philippines
2009-06-01
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doaj-3ec8d40aee844f519eba2ac702cddddd2020-11-24T20:44:53ZengUniversity of the PhilippinesScience Diliman0115-78092012-08182009-06-012112936Ion Chromatographic Method with Post-Column Fuchsin Reaction for Measurement of Bromate in Chlorinated WaterHomer C. GenuinoMaria Pythias B. EspinoAn ion chromatographic method that employs a post-column reaction with fuchsin and spectrophotometric detection was optimized for measuring bromate (BrO3-) in water. BrO3- is converted to Br2 by sodium metabisulfite and then reacted with acidic fuchsin to form a red-colored product that strongly absorbs at 530 nm. The reaction of BrO3- and fuchsin reagent is optimum at pH 3.5 and 65 oC. The method has a limit of quantitation of 4.5 µg L-1 and is linear up to 150 µg L-1 BrO3-. Recoveries from spiked samples were high ranging from 95 to 102 % using external standard calibration and 87 to 103 % using standard addition method. Intra-batch and inter-batch reproducibility studies of the method resulted to RSD values ranging from 0.62 to 2.01 % and percent relative error of 0.12 to 2.94 % for BrO3- concentrations of 10 µg L-1 and 50 µg L-1. This method is free of interferences from common inorganic anions at levels typically found in chlorinated tap drinking water without preconcentration. The optimized method can be applied to trace analysis of bromate in chlorinated tap drinking water samples.http://journals.upd.edu.ph/index.php/sciencediliman/article/view/1518bromatefuchsinchlorinated waterpost-column reactionion chromatography |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Homer C. Genuino Maria Pythias B. Espino |
spellingShingle |
Homer C. Genuino Maria Pythias B. Espino Ion Chromatographic Method with Post-Column Fuchsin Reaction for Measurement of Bromate in Chlorinated Water Science Diliman bromate fuchsin chlorinated water post-column reaction ion chromatography |
author_facet |
Homer C. Genuino Maria Pythias B. Espino |
author_sort |
Homer C. Genuino |
title |
Ion Chromatographic Method with Post-Column Fuchsin Reaction for Measurement of Bromate in Chlorinated Water |
title_short |
Ion Chromatographic Method with Post-Column Fuchsin Reaction for Measurement of Bromate in Chlorinated Water |
title_full |
Ion Chromatographic Method with Post-Column Fuchsin Reaction for Measurement of Bromate in Chlorinated Water |
title_fullStr |
Ion Chromatographic Method with Post-Column Fuchsin Reaction for Measurement of Bromate in Chlorinated Water |
title_full_unstemmed |
Ion Chromatographic Method with Post-Column Fuchsin Reaction for Measurement of Bromate in Chlorinated Water |
title_sort |
ion chromatographic method with post-column fuchsin reaction for measurement of bromate in chlorinated water |
publisher |
University of the Philippines |
series |
Science Diliman |
issn |
0115-7809 2012-0818 |
publishDate |
2009-06-01 |
description |
An ion chromatographic method that employs a post-column reaction with fuchsin and spectrophotometric detection was optimized for measuring bromate (BrO3-) in water. BrO3- is converted to Br2 by sodium metabisulfite and then reacted with acidic fuchsin to form a red-colored product that strongly absorbs at 530 nm. The reaction of BrO3- and fuchsin reagent is optimum at pH 3.5 and 65 oC. The method has a limit of quantitation of 4.5 µg L-1 and is linear up to 150 µg L-1 BrO3-. Recoveries from spiked samples were high ranging from 95 to 102 % using external standard calibration and 87 to 103 % using standard addition method. Intra-batch and inter-batch reproducibility studies of the method resulted to RSD values ranging from 0.62 to 2.01 % and percent relative error of 0.12 to 2.94 % for BrO3- concentrations of 10 µg L-1 and 50 µg L-1. This method is free of interferences from common inorganic anions at levels typically found in chlorinated tap drinking water without preconcentration. The optimized method can be applied to trace analysis of bromate in chlorinated tap drinking water samples. |
topic |
bromate fuchsin chlorinated water post-column reaction ion chromatography |
url |
http://journals.upd.edu.ph/index.php/sciencediliman/article/view/1518 |
work_keys_str_mv |
AT homercgenuino ionchromatographicmethodwithpostcolumnfuchsinreactionformeasurementofbromateinchlorinatedwater AT mariapythiasbespino ionchromatographicmethodwithpostcolumnfuchsinreactionformeasurementofbromateinchlorinatedwater |
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