Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons

Oil spill pollution remains a serious concern in marine environments and the development of effective oil bioremediation techniques are vital. This work is aimed at developing an autochthonous hydrocarbon-degrading consortium with bacterial strains with high potential for hydrocarbons degradation, o...

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Main Authors: Rafaela Perdigão, C. Marisa R. Almeida, Filipa Santos, Maria F. Carvalho, Ana P. Mucha
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Water
Subjects:
Online Access:https://www.mdpi.com/2073-4441/13/1/66
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spelling doaj-3edd759b20704fc39a7f80c5458085b62021-01-01T00:00:51ZengMDPI AGWater2073-44412021-12-0113666610.3390/w13010066Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum HydrocarbonsRafaela Perdigão0C. Marisa R. Almeida1Filipa Santos2Maria F. Carvalho3Ana P. Mucha4Interdisciplinary Centre of Marine and Environmental Research of the University of Porto, Novo Edifício do Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, S/N, 4450-208 Matosinhos, PortugalInterdisciplinary Centre of Marine and Environmental Research of the University of Porto, Novo Edifício do Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, S/N, 4450-208 Matosinhos, PortugalInterdisciplinary Centre of Marine and Environmental Research of the University of Porto, Novo Edifício do Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, S/N, 4450-208 Matosinhos, PortugalInterdisciplinary Centre of Marine and Environmental Research of the University of Porto, Novo Edifício do Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, S/N, 4450-208 Matosinhos, PortugalInterdisciplinary Centre of Marine and Environmental Research of the University of Porto, Novo Edifício do Terminal de Cruzeiros do Porto de Leixões, Avenida General Norton de Matos, S/N, 4450-208 Matosinhos, PortugalOil spill pollution remains a serious concern in marine environments and the development of effective oil bioremediation techniques are vital. This work is aimed at developing an autochthonous hydrocarbon-degrading consortium with bacterial strains with high potential for hydrocarbons degradation, optimizing first the growth conditions for the consortium, and then testing its hydrocarbon-degrading performance in microcosm bioremediation experiments. Bacterial strains, previously isolated from a sediment and cryopreserved in a georeferenced microbial bank, belonged to the genera <i>Pseudomonas</i>, <i>Rhodococcus</i> and <i>Acinetobacter</i>. Microcosms were assembled with natural seawater and petroleum, for testing: natural attenuation (NA); biostimulation (BS) (nutrients addition); bioaugmentation with inoculum pre-grown in petroleum (BA/P) and bioaugmentation with inoculum pre-grown in acetate (BA/A). After 15 days, a clear blending of petroleum with seawater was observed in BS, BA/P and BA/A but not in NA. Acetate was the best substrate for consortium growth. BA/A showed the highest hydrocarbons degradation (66%). All bacterial strains added as inoculum were recovered at the end of the experiment. This study provides an insight into the capacity of autochthonous communities to degrade hydrocarbons and on the use of alternative carbon sources for bacterial biomass growth for the development of bioremediation products to respond to oil spills.https://www.mdpi.com/2073-4441/13/1/66autochthonous bacteriaoil spillsbioaugmentationbiostimulationpetroleumbioremediation
collection DOAJ
language English
format Article
sources DOAJ
author Rafaela Perdigão
C. Marisa R. Almeida
Filipa Santos
Maria F. Carvalho
Ana P. Mucha
spellingShingle Rafaela Perdigão
C. Marisa R. Almeida
Filipa Santos
Maria F. Carvalho
Ana P. Mucha
Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons
Water
autochthonous bacteria
oil spills
bioaugmentation
biostimulation
petroleum
bioremediation
author_facet Rafaela Perdigão
C. Marisa R. Almeida
Filipa Santos
Maria F. Carvalho
Ana P. Mucha
author_sort Rafaela Perdigão
title Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons
title_short Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons
title_full Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons
title_fullStr Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons
title_full_unstemmed Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons
title_sort optimization of an autochthonous bacterial consortium obtained from beach sediments for bioremediation of petroleum hydrocarbons
publisher MDPI AG
series Water
issn 2073-4441
publishDate 2021-12-01
description Oil spill pollution remains a serious concern in marine environments and the development of effective oil bioremediation techniques are vital. This work is aimed at developing an autochthonous hydrocarbon-degrading consortium with bacterial strains with high potential for hydrocarbons degradation, optimizing first the growth conditions for the consortium, and then testing its hydrocarbon-degrading performance in microcosm bioremediation experiments. Bacterial strains, previously isolated from a sediment and cryopreserved in a georeferenced microbial bank, belonged to the genera <i>Pseudomonas</i>, <i>Rhodococcus</i> and <i>Acinetobacter</i>. Microcosms were assembled with natural seawater and petroleum, for testing: natural attenuation (NA); biostimulation (BS) (nutrients addition); bioaugmentation with inoculum pre-grown in petroleum (BA/P) and bioaugmentation with inoculum pre-grown in acetate (BA/A). After 15 days, a clear blending of petroleum with seawater was observed in BS, BA/P and BA/A but not in NA. Acetate was the best substrate for consortium growth. BA/A showed the highest hydrocarbons degradation (66%). All bacterial strains added as inoculum were recovered at the end of the experiment. This study provides an insight into the capacity of autochthonous communities to degrade hydrocarbons and on the use of alternative carbon sources for bacterial biomass growth for the development of bioremediation products to respond to oil spills.
topic autochthonous bacteria
oil spills
bioaugmentation
biostimulation
petroleum
bioremediation
url https://www.mdpi.com/2073-4441/13/1/66
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