Effects of honey, glucose, and fructose on the enamel demineralization depth
Background/purpose: Caries prevention is an important strategy in many countries. Honey has antibacterial activity against cariogenic bacteria and therefore may have less caries activity than other sugars. This study was conducted to compare the cariogenic effect of honey with those of glucose and f...
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doaj-3ef25b66b0e3474aad92b0471e7fe39d2020-11-24T22:02:32ZengElsevierJournal of Dental Sciences1991-79022013-06-018214715010.1016/j.jds.2012.02.004Effects of honey, glucose, and fructose on the enamel demineralization depthFatemeh Ahmadi-Motamayel0Loghman Rezaei-Soufi1Leila Kiani2Mohammad Yousef Alikhani3Jalal Poorolajal4Masoud Moghadam5Research Center for Molecular Medicine, Department of Oral Medicine, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, IranDepartment of Operative Dentistry, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, IranDepartment of Oral Medicine, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, IranDepartment of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IranResearch Center for Health Sciences, Department of Epidemiology and Biostatistics, School of Public Health, Hamadan University of Medical Sciences, Hamadan, IranDepartment of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IranBackground/purpose: Caries prevention is an important strategy in many countries. Honey has antibacterial activity against cariogenic bacteria and therefore may have less caries activity than other sugars. This study was conducted to compare the cariogenic effect of honey with those of glucose and fructose. Materials and methods: In this in vitro study, 36 extracted caries-free human premolars were collected and prepared following a multistage laboratory process. Then, the teeth were randomly divided into three groups. Each group was put into separate tubes containing different solutions of honey, fructose, and glucose in a brain-heart infusion broth environment. About 1.5 × 108 cells of Streptococcus mutans (equal to 0.5 McFarland units) were added to each tube. Every other day, 2 ml of the solution were replaced by 2 ml of a previously prepared solution for 21 days. Teeth were sectioned buccolingually using a diamond-saw microtome. The demineralization depth of each section was measured at three points, and the average of three representative measurements was considered the lesion depth. Results: Mean ± SD demineralization depths related to honey, glucose, and fructose were 160.1 ± 59.82 μm, 245.98 ± 96.13 μm, and 195.98 ± 47.53 μm, respectively. Differences among the three means were statistically significant. Conclusion: The results of this study demonstrated that honey had less caries activity than the other sugars. However, further evidence is required to detect the active components and mechanisms by which it reduces demineralization and to demonstrate whether this food has any clinical application for preventing and reducing dental caries.http://www.sciencedirect.com/science/article/pii/S1991790212001687enamel demineralizationfructoseglucosehoney |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fatemeh Ahmadi-Motamayel Loghman Rezaei-Soufi Leila Kiani Mohammad Yousef Alikhani Jalal Poorolajal Masoud Moghadam |
spellingShingle |
Fatemeh Ahmadi-Motamayel Loghman Rezaei-Soufi Leila Kiani Mohammad Yousef Alikhani Jalal Poorolajal Masoud Moghadam Effects of honey, glucose, and fructose on the enamel demineralization depth Journal of Dental Sciences enamel demineralization fructose glucose honey |
author_facet |
Fatemeh Ahmadi-Motamayel Loghman Rezaei-Soufi Leila Kiani Mohammad Yousef Alikhani Jalal Poorolajal Masoud Moghadam |
author_sort |
Fatemeh Ahmadi-Motamayel |
title |
Effects of honey, glucose, and fructose on the enamel demineralization depth |
title_short |
Effects of honey, glucose, and fructose on the enamel demineralization depth |
title_full |
Effects of honey, glucose, and fructose on the enamel demineralization depth |
title_fullStr |
Effects of honey, glucose, and fructose on the enamel demineralization depth |
title_full_unstemmed |
Effects of honey, glucose, and fructose on the enamel demineralization depth |
title_sort |
effects of honey, glucose, and fructose on the enamel demineralization depth |
publisher |
Elsevier |
series |
Journal of Dental Sciences |
issn |
1991-7902 |
publishDate |
2013-06-01 |
description |
Background/purpose: Caries prevention is an important strategy in many countries. Honey has antibacterial activity against cariogenic bacteria and therefore may have less caries activity than other sugars. This study was conducted to compare the cariogenic effect of honey with those of glucose and fructose.
Materials and methods: In this in vitro study, 36 extracted caries-free human premolars were collected and prepared following a multistage laboratory process. Then, the teeth were randomly divided into three groups. Each group was put into separate tubes containing different solutions of honey, fructose, and glucose in a brain-heart infusion broth environment. About 1.5 × 108 cells of Streptococcus mutans (equal to 0.5 McFarland units) were added to each tube. Every other day, 2 ml of the solution were replaced by 2 ml of a previously prepared solution for 21 days. Teeth were sectioned buccolingually using a diamond-saw microtome. The demineralization depth of each section was measured at three points, and the average of three representative measurements was considered the lesion depth.
Results: Mean ± SD demineralization depths related to honey, glucose, and fructose were 160.1 ± 59.82 μm, 245.98 ± 96.13 μm, and 195.98 ± 47.53 μm, respectively. Differences among the three means were statistically significant.
Conclusion: The results of this study demonstrated that honey had less caries activity than the other sugars. However, further evidence is required to detect the active components and mechanisms by which it reduces demineralization and to demonstrate whether this food has any clinical application for preventing and reducing dental caries. |
topic |
enamel demineralization fructose glucose honey |
url |
http://www.sciencedirect.com/science/article/pii/S1991790212001687 |
work_keys_str_mv |
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