Selective Flamingo Medium for the Isolation of <i>Aspergillus fumigatus</i>

For various studies in the clinic as well as the environment, it is essential to be able to selectively isolate <i>Aspergillus fumigatus</i> from samples containing bacteria as well as various other fungi (mainly Mucorales). Six agar media were compared for effectiveness in selectively i...

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Main Authors: Jianhua Zhang, Alfons J. M. Debets, Paul E. Verweij, Sijmen E. Schoustra
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:Microorganisms
Subjects:
Online Access:https://www.mdpi.com/2076-2607/9/6/1155
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spelling doaj-401d6dc24b864e21ac0d84e947cbc9e62021-06-01T01:23:57ZengMDPI AGMicroorganisms2076-26072021-05-0191155115510.3390/microorganisms9061155Selective Flamingo Medium for the Isolation of <i>Aspergillus fumigatus</i>Jianhua Zhang0Alfons J. M. Debets1Paul E. Verweij2Sijmen E. Schoustra3Laboratory for Genetics, Wageningen University and Research, 6708 PB Wageningen, The NetherlandsLaboratory for Genetics, Wageningen University and Research, 6708 PB Wageningen, The NetherlandsDepartment of Medical Microbiology, Radboud University Medical Center, 6500 HB Nijmegen, The NetherlandsLaboratory for Genetics, Wageningen University and Research, 6708 PB Wageningen, The NetherlandsFor various studies in the clinic as well as the environment, it is essential to be able to selectively isolate <i>Aspergillus fumigatus</i> from samples containing bacteria as well as various other fungi (mainly Mucorales). Six agar media were compared for effectiveness in selectively isolating <i>Aspergillus fumigatus</i> from agricultural plant waste, woodchip waste, green waste, soil, grass and air samples collected in The Netherlands at a 48 °C incubation. The Flamingo Medium incubated at 48 °C, provided the most effective condition for the isolation of <i>A. fumigatus</i> from environmental samples, since it effectively inhibited the growth of competing fungi (mainly Mucorales) present in the environmental samples. Flamingo Medium reduced the number of colonies of Mucorales species by 95% and recovered an average of 20−30% more <i>A. fumigatus</i> colonies compared to the other media. We further confirmed that Flamingo Medium can inhibit the growth of clinical Mucorales, which occasionally present in patient’s tissue and can also be used for clinical applications. We suggest the use of Flamingo Medium as an efficient method for the study of <i>A. fumigatus</i> from important environmental niches for which there is increasing interest. Additionally, it can also be used in the clinic to isolate <i>A. fumigatus</i> especially from tissue contaminated with Mucorales.https://www.mdpi.com/2076-2607/9/6/1155<i>Aspergillus fumigatus</i>environmental samplesselective mediumMucorales
collection DOAJ
language English
format Article
sources DOAJ
author Jianhua Zhang
Alfons J. M. Debets
Paul E. Verweij
Sijmen E. Schoustra
spellingShingle Jianhua Zhang
Alfons J. M. Debets
Paul E. Verweij
Sijmen E. Schoustra
Selective Flamingo Medium for the Isolation of <i>Aspergillus fumigatus</i>
Microorganisms
<i>Aspergillus fumigatus</i>
environmental samples
selective medium
Mucorales
author_facet Jianhua Zhang
Alfons J. M. Debets
Paul E. Verweij
Sijmen E. Schoustra
author_sort Jianhua Zhang
title Selective Flamingo Medium for the Isolation of <i>Aspergillus fumigatus</i>
title_short Selective Flamingo Medium for the Isolation of <i>Aspergillus fumigatus</i>
title_full Selective Flamingo Medium for the Isolation of <i>Aspergillus fumigatus</i>
title_fullStr Selective Flamingo Medium for the Isolation of <i>Aspergillus fumigatus</i>
title_full_unstemmed Selective Flamingo Medium for the Isolation of <i>Aspergillus fumigatus</i>
title_sort selective flamingo medium for the isolation of <i>aspergillus fumigatus</i>
publisher MDPI AG
series Microorganisms
issn 2076-2607
publishDate 2021-05-01
description For various studies in the clinic as well as the environment, it is essential to be able to selectively isolate <i>Aspergillus fumigatus</i> from samples containing bacteria as well as various other fungi (mainly Mucorales). Six agar media were compared for effectiveness in selectively isolating <i>Aspergillus fumigatus</i> from agricultural plant waste, woodchip waste, green waste, soil, grass and air samples collected in The Netherlands at a 48 °C incubation. The Flamingo Medium incubated at 48 °C, provided the most effective condition for the isolation of <i>A. fumigatus</i> from environmental samples, since it effectively inhibited the growth of competing fungi (mainly Mucorales) present in the environmental samples. Flamingo Medium reduced the number of colonies of Mucorales species by 95% and recovered an average of 20−30% more <i>A. fumigatus</i> colonies compared to the other media. We further confirmed that Flamingo Medium can inhibit the growth of clinical Mucorales, which occasionally present in patient’s tissue and can also be used for clinical applications. We suggest the use of Flamingo Medium as an efficient method for the study of <i>A. fumigatus</i> from important environmental niches for which there is increasing interest. Additionally, it can also be used in the clinic to isolate <i>A. fumigatus</i> especially from tissue contaminated with Mucorales.
topic <i>Aspergillus fumigatus</i>
environmental samples
selective medium
Mucorales
url https://www.mdpi.com/2076-2607/9/6/1155
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