Xanthophylls are preferentially taken up compared with β-carotene by retinal cells via a SRBI-dependent mechanism*1
The purpose of this study was to investigate the mechanisms by which carotenoids [xanthophylls vs. β-carotene(β-C)] are taken up by retinal pigment epithelial (RPE) cells. The human RPE cell line, ARPE-19, was used. When ARPE-19 cells were fully differentiated (7–9 weeks), the xanthophylls lutein (L...
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doaj-41a99f00ded8435aa59e7d11570d97582021-04-28T05:58:34ZengElsevierJournal of Lipid Research0022-22752008-08-0149817151724Xanthophylls are preferentially taken up compared with β-carotene by retinal cells via a SRBI-dependent mechanism*1Alexandrine During0Sundari Doraiswamy1Earl H. Harrison2Phytonutrients Laboratory, U.S. Department of Agriculture-Agricultural Research Service, Beltsville Human Nutrition Research Center, Beltsville, MD 20705; Laboratoire de Biochimie Cellulaire, Université Catholique de Louvain, Louvain-La-Neuve B1348, BelgiumPhytonutrients Laboratory, U.S. Department of Agriculture-Agricultural Research Service, Beltsville Human Nutrition Research Center, Beltsville, MD 20705Phytonutrients Laboratory, U.S. Department of Agriculture-Agricultural Research Service, Beltsville Human Nutrition Research Center, Beltsville, MD 20705; Department of Human Nutrition, Ohio State University, Columbus, OH 43210The purpose of this study was to investigate the mechanisms by which carotenoids [xanthophylls vs. β-carotene(β-C)] are taken up by retinal pigment epithelial (RPE) cells. The human RPE cell line, ARPE-19, was used. When ARPE-19 cells were fully differentiated (7–9 weeks), the xanthophylls lutein (LUT) and zeaxanthin (ZEA) were taken up by cells to an extent 2-fold higher than β-C (P < 0.05). At 9 weeks, cellular uptakes were 1.6, 2.5, and 3.2%, respectively, for β-C, LUT, and ZEA. Similar extents were observed when carotenoids were delivered in either Tween 40 or “chylomicrons” produced by Caco-2 cells. Differentiated ARPE-19 cells did not exhibit any detectable β-C 15,15′-oxygenase activity or convert exogenous β-C into vitamin A. When using specific antibodies against the lipid transporters cluster determinant 36 (CD36) and scavenger receptor class B type I (SR-BI), cellular uptake of β-C and ZEA were significantly decreased (40–60%) with anti-SR-BI but not with anti-CD36. Small interfering RNA transfection for SR-BI led to marked knockdown of SR-BI protein expression (∼90%), which resulted in decreased β-C and ZEA uptakes by 51% and 87%, respectively. Thus, the present data show that RPE cells preferentially take up xanthophylls versus the carotene by a process that appears to be entirely SR-BI-dependent for ZEA and partly so for β-C. This mechanism may explain, in part, the preferential accumulation of xanthophylls in the macula of the retina.http://www.sciencedirect.com/science/article/pii/S0022227520346903carotenoidsxanthophyllscellular uptakescavenger receptor class B type Ismall interfering RNAsARPE-19 cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alexandrine During Sundari Doraiswamy Earl H. Harrison |
spellingShingle |
Alexandrine During Sundari Doraiswamy Earl H. Harrison Xanthophylls are preferentially taken up compared with β-carotene by retinal cells via a SRBI-dependent mechanism*1 Journal of Lipid Research carotenoids xanthophylls cellular uptake scavenger receptor class B type I small interfering RNAs ARPE-19 cells |
author_facet |
Alexandrine During Sundari Doraiswamy Earl H. Harrison |
author_sort |
Alexandrine During |
title |
Xanthophylls are preferentially taken up compared with β-carotene by retinal cells via a SRBI-dependent mechanism*1 |
title_short |
Xanthophylls are preferentially taken up compared with β-carotene by retinal cells via a SRBI-dependent mechanism*1 |
title_full |
Xanthophylls are preferentially taken up compared with β-carotene by retinal cells via a SRBI-dependent mechanism*1 |
title_fullStr |
Xanthophylls are preferentially taken up compared with β-carotene by retinal cells via a SRBI-dependent mechanism*1 |
title_full_unstemmed |
Xanthophylls are preferentially taken up compared with β-carotene by retinal cells via a SRBI-dependent mechanism*1 |
title_sort |
xanthophylls are preferentially taken up compared with β-carotene by retinal cells via a srbi-dependent mechanism*1 |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2008-08-01 |
description |
The purpose of this study was to investigate the mechanisms by which carotenoids [xanthophylls vs. β-carotene(β-C)] are taken up by retinal pigment epithelial (RPE) cells. The human RPE cell line, ARPE-19, was used. When ARPE-19 cells were fully differentiated (7–9 weeks), the xanthophylls lutein (LUT) and zeaxanthin (ZEA) were taken up by cells to an extent 2-fold higher than β-C (P < 0.05). At 9 weeks, cellular uptakes were 1.6, 2.5, and 3.2%, respectively, for β-C, LUT, and ZEA. Similar extents were observed when carotenoids were delivered in either Tween 40 or “chylomicrons” produced by Caco-2 cells. Differentiated ARPE-19 cells did not exhibit any detectable β-C 15,15′-oxygenase activity or convert exogenous β-C into vitamin A. When using specific antibodies against the lipid transporters cluster determinant 36 (CD36) and scavenger receptor class B type I (SR-BI), cellular uptake of β-C and ZEA were significantly decreased (40–60%) with anti-SR-BI but not with anti-CD36. Small interfering RNA transfection for SR-BI led to marked knockdown of SR-BI protein expression (∼90%), which resulted in decreased β-C and ZEA uptakes by 51% and 87%, respectively. Thus, the present data show that RPE cells preferentially take up xanthophylls versus the carotene by a process that appears to be entirely SR-BI-dependent for ZEA and partly so for β-C. This mechanism may explain, in part, the preferential accumulation of xanthophylls in the macula of the retina. |
topic |
carotenoids xanthophylls cellular uptake scavenger receptor class B type I small interfering RNAs ARPE-19 cells |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520346903 |
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