Evaluation of a PCR multiplex for detection and differentiation of Mycoplasma synoviae, M. gallisepticum, and M. gallisepticum strain F-vaccine

Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the mycoplasma infections of most concern for commercial poultry industry. MG infection is commonly designated as chronic respiratory disease (CRD) of chickens and infections sinusitis of turkeys. MS causes sub clinical upper respiratory...

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Main Authors: Elena Mettifogo, Melissa Buzinhani, Marcos R. Buim, Jorge Timenetsky, Antonio J. Piantino Ferreira
Format: Article
Language:English
Published: Colégio Brasileiro de Patologia Animal (CBPA) 2015-01-01
Series:Pesquisa Veterinária Brasileira
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000100013&lng=en&tlng=en
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spelling doaj-41b88b74d5494dd7b36354e5a27f735a2020-11-24T23:15:37ZengColégio Brasileiro de Patologia Animal (CBPA)Pesquisa Veterinária Brasileira1678-51502015-01-01351131810.1590/S0100-736X2015000100004S0100-736X2015000100013Evaluation of a PCR multiplex for detection and differentiation of Mycoplasma synoviae, M. gallisepticum, and M. gallisepticum strain F-vaccineElena MettifogoMelissa BuzinhaniMarcos R. BuimJorge TimenetskyAntonio J. Piantino FerreiraMycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the mycoplasma infections of most concern for commercial poultry industry. MG infection is commonly designated as chronic respiratory disease (CRD) of chickens and infections sinusitis of turkeys. MS causes sub clinical upper respiratory infection and tenosynovitis or bursitis in chickens and turkeys. The multiplex PCR was standardized to detect simultaneously the MS, MG field strains and MG F-vaccine strain specific. The generic PCR for detection of any species of Mollicutes Class was performed and compared to the multiplex PCR and to PCR using species-specific primers. A total of 129 avian tracheal swabs were collected from broiler-breeders, layer hens and broilers in seven different farms and were examined by multiplex PCR methods. The system (multiplex PCR) demonstrated to be very rapid, sensitive, and specific. Therefore, the results showed a high prevalence of MS in the flocks examined (27.9%), and indicate that the MS is a recurrent pathogen in Brazilian commercial poultry flocks.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000100013&lng=en&tlng=enGalinhaMycoplasma gallisepticumMycoplasma synoviaePCR multiplex.
collection DOAJ
language English
format Article
sources DOAJ
author Elena Mettifogo
Melissa Buzinhani
Marcos R. Buim
Jorge Timenetsky
Antonio J. Piantino Ferreira
spellingShingle Elena Mettifogo
Melissa Buzinhani
Marcos R. Buim
Jorge Timenetsky
Antonio J. Piantino Ferreira
Evaluation of a PCR multiplex for detection and differentiation of Mycoplasma synoviae, M. gallisepticum, and M. gallisepticum strain F-vaccine
Pesquisa Veterinária Brasileira
Galinha
Mycoplasma gallisepticum
Mycoplasma synoviae
PCR multiplex.
author_facet Elena Mettifogo
Melissa Buzinhani
Marcos R. Buim
Jorge Timenetsky
Antonio J. Piantino Ferreira
author_sort Elena Mettifogo
title Evaluation of a PCR multiplex for detection and differentiation of Mycoplasma synoviae, M. gallisepticum, and M. gallisepticum strain F-vaccine
title_short Evaluation of a PCR multiplex for detection and differentiation of Mycoplasma synoviae, M. gallisepticum, and M. gallisepticum strain F-vaccine
title_full Evaluation of a PCR multiplex for detection and differentiation of Mycoplasma synoviae, M. gallisepticum, and M. gallisepticum strain F-vaccine
title_fullStr Evaluation of a PCR multiplex for detection and differentiation of Mycoplasma synoviae, M. gallisepticum, and M. gallisepticum strain F-vaccine
title_full_unstemmed Evaluation of a PCR multiplex for detection and differentiation of Mycoplasma synoviae, M. gallisepticum, and M. gallisepticum strain F-vaccine
title_sort evaluation of a pcr multiplex for detection and differentiation of mycoplasma synoviae, m. gallisepticum, and m. gallisepticum strain f-vaccine
publisher Colégio Brasileiro de Patologia Animal (CBPA)
series Pesquisa Veterinária Brasileira
issn 1678-5150
publishDate 2015-01-01
description Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the mycoplasma infections of most concern for commercial poultry industry. MG infection is commonly designated as chronic respiratory disease (CRD) of chickens and infections sinusitis of turkeys. MS causes sub clinical upper respiratory infection and tenosynovitis or bursitis in chickens and turkeys. The multiplex PCR was standardized to detect simultaneously the MS, MG field strains and MG F-vaccine strain specific. The generic PCR for detection of any species of Mollicutes Class was performed and compared to the multiplex PCR and to PCR using species-specific primers. A total of 129 avian tracheal swabs were collected from broiler-breeders, layer hens and broilers in seven different farms and were examined by multiplex PCR methods. The system (multiplex PCR) demonstrated to be very rapid, sensitive, and specific. Therefore, the results showed a high prevalence of MS in the flocks examined (27.9%), and indicate that the MS is a recurrent pathogen in Brazilian commercial poultry flocks.
topic Galinha
Mycoplasma gallisepticum
Mycoplasma synoviae
PCR multiplex.
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000100013&lng=en&tlng=en
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