Growth of <i>Staphylococcus epidermidis</i> on the Surface of Teatcups from Milking Parlours

The growth of two <i>Staphylococcus epidermidis</i> isolates (one biofilm-forming and one not) on teatcups for cattle (made of rubber) or sheep (made of silicone) were assessed in nine multiplicates for 24 h post-smearing on the teatcup surface. Staphylococci were smeared on an area of 0...

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Main Authors: Eleni I. Katsarou, Angeliki I. Katsafadou, Theodoros Karakasidis, Dimitris C. Chatzopoulos, Natalia G. C. Vasileiou, Daphne T. Lianou, Vasia S. Mavrogianni, Efthymia Petinaki, George C. Fthenakis
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Microorganisms
Subjects:
Online Access:https://www.mdpi.com/2076-2607/9/4/852
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spelling doaj-427aea3d44cf4ec9be8701b1e00bc0362021-04-15T23:06:00ZengMDPI AGMicroorganisms2076-26072021-04-01985285210.3390/microorganisms9040852Growth of <i>Staphylococcus epidermidis</i> on the Surface of Teatcups from Milking ParloursEleni I. Katsarou0Angeliki I. Katsafadou1Theodoros Karakasidis2Dimitris C. Chatzopoulos3Natalia G. C. Vasileiou4Daphne T. Lianou5Vasia S. Mavrogianni6Efthymia Petinaki7George C. Fthenakis8Veterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceFaculty of Public and Integrated (One) Health, University of Thessaly, 43100 Karditsa, GreeceFaculty of Physics, University of Thessaly, 35131 Lamia, GreeceFaculty of Public and Integrated (One) Health, University of Thessaly, 43100 Karditsa, GreeceFaculty of Animal Science, University of Thessaly, 41110 Larisa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceUniversity Hospital, University of Thessaly, 41110 Larisa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceThe growth of two <i>Staphylococcus epidermidis</i> isolates (one biofilm-forming and one not) on teatcups for cattle (made of rubber) or sheep (made of silicone) were assessed in nine multiplicates for 24 h post-smearing on the teatcup surface. Staphylococci were smeared on an area of 0.0003142 m<sup>2</sup> on the material and their growth and expansion further on were monitored for 24 h. There were no differences in the frequency of recoveries between the two isolates (<i>p </i>> 0.82 for all comparisons). There were more recoveries from sheep teatcups than from cattle teatcups: 1280/1728 (74.1%) versus 942/1728 (54.5%), for both isolates (<i>p </i>< 0.0001). Significance was observed only 6 h to 15 h after smearing (<i>p </i>< 0.0001 for all comparisons). The median speed of linear dissemination of the isolates was 0.00000021 m s<sup>−1</sup> on cattle teatcups and 0.00000033 m s<sup>−1</sup> on sheep teatcups (<i>p </i>< 0.0001). The increased growth and faster expansion of staphylococci on silicone teatcups raise important points from a clinical viewpoint. The model could be used in the testing of staphylococcal growth in the material of milking parlours in various conditions.https://www.mdpi.com/2076-2607/9/4/852biofilmcattlemastitismilking parlourmodelrubber
collection DOAJ
language English
format Article
sources DOAJ
author Eleni I. Katsarou
Angeliki I. Katsafadou
Theodoros Karakasidis
Dimitris C. Chatzopoulos
Natalia G. C. Vasileiou
Daphne T. Lianou
Vasia S. Mavrogianni
Efthymia Petinaki
George C. Fthenakis
spellingShingle Eleni I. Katsarou
Angeliki I. Katsafadou
Theodoros Karakasidis
Dimitris C. Chatzopoulos
Natalia G. C. Vasileiou
Daphne T. Lianou
Vasia S. Mavrogianni
Efthymia Petinaki
George C. Fthenakis
Growth of <i>Staphylococcus epidermidis</i> on the Surface of Teatcups from Milking Parlours
Microorganisms
biofilm
cattle
mastitis
milking parlour
model
rubber
author_facet Eleni I. Katsarou
Angeliki I. Katsafadou
Theodoros Karakasidis
Dimitris C. Chatzopoulos
Natalia G. C. Vasileiou
Daphne T. Lianou
Vasia S. Mavrogianni
Efthymia Petinaki
George C. Fthenakis
author_sort Eleni I. Katsarou
title Growth of <i>Staphylococcus epidermidis</i> on the Surface of Teatcups from Milking Parlours
title_short Growth of <i>Staphylococcus epidermidis</i> on the Surface of Teatcups from Milking Parlours
title_full Growth of <i>Staphylococcus epidermidis</i> on the Surface of Teatcups from Milking Parlours
title_fullStr Growth of <i>Staphylococcus epidermidis</i> on the Surface of Teatcups from Milking Parlours
title_full_unstemmed Growth of <i>Staphylococcus epidermidis</i> on the Surface of Teatcups from Milking Parlours
title_sort growth of <i>staphylococcus epidermidis</i> on the surface of teatcups from milking parlours
publisher MDPI AG
series Microorganisms
issn 2076-2607
publishDate 2021-04-01
description The growth of two <i>Staphylococcus epidermidis</i> isolates (one biofilm-forming and one not) on teatcups for cattle (made of rubber) or sheep (made of silicone) were assessed in nine multiplicates for 24 h post-smearing on the teatcup surface. Staphylococci were smeared on an area of 0.0003142 m<sup>2</sup> on the material and their growth and expansion further on were monitored for 24 h. There were no differences in the frequency of recoveries between the two isolates (<i>p </i>> 0.82 for all comparisons). There were more recoveries from sheep teatcups than from cattle teatcups: 1280/1728 (74.1%) versus 942/1728 (54.5%), for both isolates (<i>p </i>< 0.0001). Significance was observed only 6 h to 15 h after smearing (<i>p </i>< 0.0001 for all comparisons). The median speed of linear dissemination of the isolates was 0.00000021 m s<sup>−1</sup> on cattle teatcups and 0.00000033 m s<sup>−1</sup> on sheep teatcups (<i>p </i>< 0.0001). The increased growth and faster expansion of staphylococci on silicone teatcups raise important points from a clinical viewpoint. The model could be used in the testing of staphylococcal growth in the material of milking parlours in various conditions.
topic biofilm
cattle
mastitis
milking parlour
model
rubber
url https://www.mdpi.com/2076-2607/9/4/852
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