Growth of <i>Staphylococcus epidermidis</i> on the Surface of Teatcups from Milking Parlours
The growth of two <i>Staphylococcus epidermidis</i> isolates (one biofilm-forming and one not) on teatcups for cattle (made of rubber) or sheep (made of silicone) were assessed in nine multiplicates for 24 h post-smearing on the teatcup surface. Staphylococci were smeared on an area of 0...
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doaj-427aea3d44cf4ec9be8701b1e00bc0362021-04-15T23:06:00ZengMDPI AGMicroorganisms2076-26072021-04-01985285210.3390/microorganisms9040852Growth of <i>Staphylococcus epidermidis</i> on the Surface of Teatcups from Milking ParloursEleni I. Katsarou0Angeliki I. Katsafadou1Theodoros Karakasidis2Dimitris C. Chatzopoulos3Natalia G. C. Vasileiou4Daphne T. Lianou5Vasia S. Mavrogianni6Efthymia Petinaki7George C. Fthenakis8Veterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceFaculty of Public and Integrated (One) Health, University of Thessaly, 43100 Karditsa, GreeceFaculty of Physics, University of Thessaly, 35131 Lamia, GreeceFaculty of Public and Integrated (One) Health, University of Thessaly, 43100 Karditsa, GreeceFaculty of Animal Science, University of Thessaly, 41110 Larisa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceUniversity Hospital, University of Thessaly, 41110 Larisa, GreeceVeterinary Faculty, University of Thessaly, 43100 Karditsa, GreeceThe growth of two <i>Staphylococcus epidermidis</i> isolates (one biofilm-forming and one not) on teatcups for cattle (made of rubber) or sheep (made of silicone) were assessed in nine multiplicates for 24 h post-smearing on the teatcup surface. Staphylococci were smeared on an area of 0.0003142 m<sup>2</sup> on the material and their growth and expansion further on were monitored for 24 h. There were no differences in the frequency of recoveries between the two isolates (<i>p </i>> 0.82 for all comparisons). There were more recoveries from sheep teatcups than from cattle teatcups: 1280/1728 (74.1%) versus 942/1728 (54.5%), for both isolates (<i>p </i>< 0.0001). Significance was observed only 6 h to 15 h after smearing (<i>p </i>< 0.0001 for all comparisons). The median speed of linear dissemination of the isolates was 0.00000021 m s<sup>−1</sup> on cattle teatcups and 0.00000033 m s<sup>−1</sup> on sheep teatcups (<i>p </i>< 0.0001). The increased growth and faster expansion of staphylococci on silicone teatcups raise important points from a clinical viewpoint. The model could be used in the testing of staphylococcal growth in the material of milking parlours in various conditions.https://www.mdpi.com/2076-2607/9/4/852biofilmcattlemastitismilking parlourmodelrubber |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eleni I. Katsarou Angeliki I. Katsafadou Theodoros Karakasidis Dimitris C. Chatzopoulos Natalia G. C. Vasileiou Daphne T. Lianou Vasia S. Mavrogianni Efthymia Petinaki George C. Fthenakis |
spellingShingle |
Eleni I. Katsarou Angeliki I. Katsafadou Theodoros Karakasidis Dimitris C. Chatzopoulos Natalia G. C. Vasileiou Daphne T. Lianou Vasia S. Mavrogianni Efthymia Petinaki George C. Fthenakis Growth of <i>Staphylococcus epidermidis</i> on the Surface of Teatcups from Milking Parlours Microorganisms biofilm cattle mastitis milking parlour model rubber |
author_facet |
Eleni I. Katsarou Angeliki I. Katsafadou Theodoros Karakasidis Dimitris C. Chatzopoulos Natalia G. C. Vasileiou Daphne T. Lianou Vasia S. Mavrogianni Efthymia Petinaki George C. Fthenakis |
author_sort |
Eleni I. Katsarou |
title |
Growth of <i>Staphylococcus epidermidis</i> on the Surface of Teatcups from Milking Parlours |
title_short |
Growth of <i>Staphylococcus epidermidis</i> on the Surface of Teatcups from Milking Parlours |
title_full |
Growth of <i>Staphylococcus epidermidis</i> on the Surface of Teatcups from Milking Parlours |
title_fullStr |
Growth of <i>Staphylococcus epidermidis</i> on the Surface of Teatcups from Milking Parlours |
title_full_unstemmed |
Growth of <i>Staphylococcus epidermidis</i> on the Surface of Teatcups from Milking Parlours |
title_sort |
growth of <i>staphylococcus epidermidis</i> on the surface of teatcups from milking parlours |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-04-01 |
description |
The growth of two <i>Staphylococcus epidermidis</i> isolates (one biofilm-forming and one not) on teatcups for cattle (made of rubber) or sheep (made of silicone) were assessed in nine multiplicates for 24 h post-smearing on the teatcup surface. Staphylococci were smeared on an area of 0.0003142 m<sup>2</sup> on the material and their growth and expansion further on were monitored for 24 h. There were no differences in the frequency of recoveries between the two isolates (<i>p </i>> 0.82 for all comparisons). There were more recoveries from sheep teatcups than from cattle teatcups: 1280/1728 (74.1%) versus 942/1728 (54.5%), for both isolates (<i>p </i>< 0.0001). Significance was observed only 6 h to 15 h after smearing (<i>p </i>< 0.0001 for all comparisons). The median speed of linear dissemination of the isolates was 0.00000021 m s<sup>−1</sup> on cattle teatcups and 0.00000033 m s<sup>−1</sup> on sheep teatcups (<i>p </i>< 0.0001). The increased growth and faster expansion of staphylococci on silicone teatcups raise important points from a clinical viewpoint. The model could be used in the testing of staphylococcal growth in the material of milking parlours in various conditions. |
topic |
biofilm cattle mastitis milking parlour model rubber |
url |
https://www.mdpi.com/2076-2607/9/4/852 |
work_keys_str_mv |
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