Direct Detection of Carbapenem-Resistant Organisms from Environmental Samples Using the GeneXpert Molecular Diagnostic System

Use of the Carba-R assay for detection of carbapenem-resistant Gram-negative organisms (CROs) can provide data for implementation of a rapid infection control response to minimize the spread of CROs in the health care setting.In this pilot study, traditional culture and PCR methods were compared to...

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Main Authors: K. A. Perry, J. B. Daniels, S. C. Reddy, A. J. Kallen, A. L. Halpin, J. K. Rasheed, J. A. Noble-Wang
Format: Article
Language:English
Published: American Society for Microbiology 2018-08-01
Series:mSphere
Subjects:
PCR
Online Access:https://doi.org/10.1128/mSphere.00113-18
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spelling doaj-428158adea6a4023bed67cee7420a1682020-11-25T00:36:26ZengAmerican Society for MicrobiologymSphere2379-50422018-08-0134e00113-1810.1128/mSphere.00113-18Direct Detection of Carbapenem-Resistant Organisms from Environmental Samples Using the GeneXpert Molecular Diagnostic SystemK. A. PerryJ. B. DanielsS. C. ReddyA. J. KallenA. L. HalpinJ. K. RasheedJ. A. Noble-WangUse of the Carba-R assay for detection of carbapenem-resistant Gram-negative organisms (CROs) can provide data for implementation of a rapid infection control response to minimize the spread of CROs in the health care setting.In this pilot study, traditional culture and PCR methods were compared to the Cepheid GeneXpert IV molecular diagnostic system with the Xpert Carba-R assay (Carba-R assay) for detection of carbapenem resistance genes in primary environmental samples collected during a health care-related outbreak. Overall, traditional culture-dependent PCR and the Carba-R assay demonstrated 75% agreement. The Carba-R assay detected carbapenemase genes in five additional samples and in two samples that had additional genes when compared to culture-dependent PCR. The Carba-R assay could be useful for prioritizing further testing of environmental samples during health care-related outbreaks.https://doi.org/10.1128/mSphere.00113-18Cepheid GeneXpertPCRcarbapenem resistantcarbapenemase genes
collection DOAJ
language English
format Article
sources DOAJ
author K. A. Perry
J. B. Daniels
S. C. Reddy
A. J. Kallen
A. L. Halpin
J. K. Rasheed
J. A. Noble-Wang
spellingShingle K. A. Perry
J. B. Daniels
S. C. Reddy
A. J. Kallen
A. L. Halpin
J. K. Rasheed
J. A. Noble-Wang
Direct Detection of Carbapenem-Resistant Organisms from Environmental Samples Using the GeneXpert Molecular Diagnostic System
mSphere
Cepheid GeneXpert
PCR
carbapenem resistant
carbapenemase genes
author_facet K. A. Perry
J. B. Daniels
S. C. Reddy
A. J. Kallen
A. L. Halpin
J. K. Rasheed
J. A. Noble-Wang
author_sort K. A. Perry
title Direct Detection of Carbapenem-Resistant Organisms from Environmental Samples Using the GeneXpert Molecular Diagnostic System
title_short Direct Detection of Carbapenem-Resistant Organisms from Environmental Samples Using the GeneXpert Molecular Diagnostic System
title_full Direct Detection of Carbapenem-Resistant Organisms from Environmental Samples Using the GeneXpert Molecular Diagnostic System
title_fullStr Direct Detection of Carbapenem-Resistant Organisms from Environmental Samples Using the GeneXpert Molecular Diagnostic System
title_full_unstemmed Direct Detection of Carbapenem-Resistant Organisms from Environmental Samples Using the GeneXpert Molecular Diagnostic System
title_sort direct detection of carbapenem-resistant organisms from environmental samples using the genexpert molecular diagnostic system
publisher American Society for Microbiology
series mSphere
issn 2379-5042
publishDate 2018-08-01
description Use of the Carba-R assay for detection of carbapenem-resistant Gram-negative organisms (CROs) can provide data for implementation of a rapid infection control response to minimize the spread of CROs in the health care setting.In this pilot study, traditional culture and PCR methods were compared to the Cepheid GeneXpert IV molecular diagnostic system with the Xpert Carba-R assay (Carba-R assay) for detection of carbapenem resistance genes in primary environmental samples collected during a health care-related outbreak. Overall, traditional culture-dependent PCR and the Carba-R assay demonstrated 75% agreement. The Carba-R assay detected carbapenemase genes in five additional samples and in two samples that had additional genes when compared to culture-dependent PCR. The Carba-R assay could be useful for prioritizing further testing of environmental samples during health care-related outbreaks.
topic Cepheid GeneXpert
PCR
carbapenem resistant
carbapenemase genes
url https://doi.org/10.1128/mSphere.00113-18
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