Optimization of Cellulase Production by Aspergillus niger ITBCC L74 with Bagasse as Substrate using Response Surface Methodology
Cellulase is a very important enzyme for lignocelluloses based ethanol production. Bagasse contains mainly cellulose (57.76%), hemicellulose (12.44%), lignin (21.34%), and others (7.96%). Lignocellulosic material has been considered as the good option for cellulase production because it is cheap an...
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Bogor Agricultural University
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doaj-42f2106096644d359312395a31cb390c2021-08-05T08:22:59ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942018-10-0125310.4308/hjb.25.3.115Optimization of Cellulase Production by Aspergillus niger ITBCC L74 with Bagasse as Substrate using Response Surface MethodologyAbdullah Abdullah0Hamid Hamid1Marcelinus Christwardana2H. Hadiyanto3Department of Chemical Engineering, Diponegoro University, Jln. Prof. Soedarto, SH, Tembalang, SemarangDepartment of Chemical Engineering, Diponegoro University, Jln. Prof. Soedarto, SH, Tembalang, SemarangGraduate School of Energy and Environment, Department of New Energy Engineering, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, Seoul 01811Department of Chemical Engineering, Diponegoro University, Jln. Prof. Soedarto, SH, Tembalang, Semarang Cellulase is a very important enzyme for lignocelluloses based ethanol production. Bagasse contains mainly cellulose (57.76%), hemicellulose (12.44%), lignin (21.34%), and others (7.96%). Lignocellulosic material has been considered as the good option for cellulase production because it is cheap and already available in a huge amount. The objective of this research was to produce cellulase enzyme and to optimize it by using response surface methodology. The bagasse with water content of 80% was incubated with 2 ml inoculum of Aspergillus niger ITBCC L74 in a 250 ml Erlenmeyer flask. After reaching the specified time the enzyme was extracted and then determined for its activity. Effect of process parameters such as pH, urea and MgCl2 addition were studied. The optimal cellulase activity was achieved at urea concentration of 4.5% (w/w), MgCl2 concentration of 1 mM and pH of 3.5, with maximum enzyme activity was 0.630 U/gr. https://journal.ipb.ac.id/index.php/hayati/article/view/23824Aspergillus nigerbagassecellulaseoptimizationsolid state fermentation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Abdullah Abdullah Hamid Hamid Marcelinus Christwardana H. Hadiyanto |
spellingShingle |
Abdullah Abdullah Hamid Hamid Marcelinus Christwardana H. Hadiyanto Optimization of Cellulase Production by Aspergillus niger ITBCC L74 with Bagasse as Substrate using Response Surface Methodology Hayati Journal of Biosciences Aspergillus niger bagasse cellulase optimization solid state fermentation |
author_facet |
Abdullah Abdullah Hamid Hamid Marcelinus Christwardana H. Hadiyanto |
author_sort |
Abdullah Abdullah |
title |
Optimization of Cellulase Production by Aspergillus niger ITBCC L74 with Bagasse as Substrate using Response Surface Methodology |
title_short |
Optimization of Cellulase Production by Aspergillus niger ITBCC L74 with Bagasse as Substrate using Response Surface Methodology |
title_full |
Optimization of Cellulase Production by Aspergillus niger ITBCC L74 with Bagasse as Substrate using Response Surface Methodology |
title_fullStr |
Optimization of Cellulase Production by Aspergillus niger ITBCC L74 with Bagasse as Substrate using Response Surface Methodology |
title_full_unstemmed |
Optimization of Cellulase Production by Aspergillus niger ITBCC L74 with Bagasse as Substrate using Response Surface Methodology |
title_sort |
optimization of cellulase production by aspergillus niger itbcc l74 with bagasse as substrate using response surface methodology |
publisher |
Bogor Agricultural University |
series |
Hayati Journal of Biosciences |
issn |
1978-3019 2086-4094 |
publishDate |
2018-10-01 |
description |
Cellulase is a very important enzyme for lignocelluloses based ethanol production. Bagasse contains mainly cellulose (57.76%), hemicellulose (12.44%), lignin (21.34%), and others (7.96%). Lignocellulosic material has been considered as the good option for cellulase production because it is cheap and already available in a huge amount. The objective of this research was to produce cellulase enzyme and to optimize it by using response surface methodology. The bagasse with water content of 80% was incubated with 2 ml inoculum of Aspergillus niger ITBCC L74 in a 250 ml Erlenmeyer flask. After reaching the specified time the enzyme was extracted and then determined for its activity. Effect of process parameters such as pH, urea and MgCl2 addition were studied. The optimal cellulase activity was achieved at urea concentration of 4.5% (w/w), MgCl2 concentration of 1 mM and pH of 3.5, with maximum enzyme activity was 0.630 U/gr.
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topic |
Aspergillus niger bagasse cellulase optimization solid state fermentation |
url |
https://journal.ipb.ac.id/index.php/hayati/article/view/23824 |
work_keys_str_mv |
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