Modern Approaches to the Methods of Creating of Phage Basis for Treatment-Prophylactic a Drug of Bacteriophages Pseudomonas aeruginosa

• Main Authors: O. A. Polygach, N. N. Voroshilov, N. V. Tikunova, V. V. Morozova, A. Y. Tikunov, B. N. Кrylov, A. A. Yunusova, A. N. Dabizheva
• Format: Article
• Language: Russian
• Published: Numikom LLC 2018-04-01
• Series: Эпидемиология и вакцинопрофилактика
• Subjects: bacteria p. aeruginosa; bacteriophages p. aeruginosa; the bacteriophage preparation; antibacterial activity
• Online Access: https://www.epidemvac.ru/jour/article/view/428

Therapeutic and prophylactic preparations of bacteriophages are considered as an effective modern means of antimicrobial therapy of Pseudomonas aeruginosa infection. Bacteriophages that are part  of the drugs must be certified for confirmation of their lytic and  genetic properties, which ensures the safety and effectiveness of the  use of drugs. In our study, biological and morphological  properties were studied for drug design, the spectrum of  antibacterial activity of bacteriophages isolated in various regions of  Russia was determined. The most important stage of the work was  research on ensuring genetic safety of bacteriophage preparations.  As a result, a composition of 6 strains of lytic bacteriophages of P.  aeruginosa (PaUfa №№ 1, 2, 3, 4, 6, 7) composed of the  morphology of the virions to the families Podoviridae and Myoviridae, the order of Caudovirales, phylogenetic genetic groups phiKMV- likevirus , N4-likevirus, PB1-likevirus. The composition has a wide  spectrum of antibacterial activity (90.7%) with respect to 818  collisional, epidemiologically significant hospital and clinical strains of P. aeruginosa bacteria, and also quite versatile for various remote  territories of Russia and CIS countries.