Docosahexaenoic acid transfer into human milk after dietary supplementation: a randomized clinical trial

Docosahexaenoic acid (DHA) is important for infant development. The DHA transfer from maternal diet into human milk has not been investigated in detail. We studied the effects of DHA supplementation on the fatty acid composition of human milk and the secretion of dietary 13C-labeled fatty acids, inc...

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Main Authors: Nataša Fidler, Thorsten Sauerwald, Anja Pohl, Hans Demmelmair, Berthold Koletzko
Format: Article
Language:English
Published: Elsevier 2000-09-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520334490
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spelling doaj-4483624c7526435cb5e877c1255a871b2021-04-27T04:45:04ZengElsevierJournal of Lipid Research0022-22752000-09-0141913761383Docosahexaenoic acid transfer into human milk after dietary supplementation: a randomized clinical trialNataša Fidler0Thorsten Sauerwald1Anja Pohl2Hans Demmelmair3Berthold Koletzko4Kinderklinik and Kinderpoliklinik, Dr. von Haunersches Kinderspital, Ludwig-Maximilian-Universitat, D-80337 Munich, GermanyKinderklinik and Kinderpoliklinik, Dr. von Haunersches Kinderspital, Ludwig-Maximilian-Universitat, D-80337 Munich, GermanyKinderklinik and Kinderpoliklinik, Dr. von Haunersches Kinderspital, Ludwig-Maximilian-Universitat, D-80337 Munich, GermanyKinderklinik and Kinderpoliklinik, Dr. von Haunersches Kinderspital, Ludwig-Maximilian-Universitat, D-80337 Munich, GermanyTo whom correspondence should be addressed.; Kinderklinik and Kinderpoliklinik, Dr. von Haunersches Kinderspital, Ludwig-Maximilian-Universitat, D-80337 Munich, GermanyDocosahexaenoic acid (DHA) is important for infant development. The DHA transfer from maternal diet into human milk has not been investigated in detail. We studied the effects of DHA supplementation on the fatty acid composition of human milk and the secretion of dietary 13C-labeled fatty acids, including DHA, into human milk. Ten lactating women were randomized to consume, from 4 to 6 weeks postpartum, an oil rich in DHA (DHASCO™, 200 mg of DHA/day) (n = 5) or a placebo oil (n = 5). Dietary intakes were followed by 7-day protocols. On study day 14 a single dose of [U-13C]DHASCO™ was given orally, milk samples were collected over 48 h, and milk production was recorded. Milk fatty acid composition was determined by gas-liquid chromatography and isotopic enrichment was determined by gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). Milk DHA content did not differ between the supplemented and placebo group at study entry (0.29 vs. 0.28 wt%, median). After 2 weeks of supplementation the milk DHA content was almost 2-fold higher in the supplemented versus placebo group (0.37 vs. 0.21 wt%, P = 0.003). Cumulative recovery of [13C]palmitic, [13C]oleic, and [13C]docosahexaenoic acids in human milk at 48 h was similar between supplemented and placebo groups (palmitic acid 7.40 vs. 8.14%, oleic acid 9.14 vs. 9.97%, and docosahexaenoic acid 9.09 vs. 8.03% of dose, respectively). Notable lower recovery was observed for [13C]myristic acid in both the supplemented and placebo groups, 0.62 versus 0.77% of dose. Dietary DHA supplementation increases the DHA content in human milk. DHA transfer from the diet into human milk is comparable to palmitic and oleic acid transfer. —Fidler, N., T. Sauerwald, A. Pohl, H. Demmelmair, and B. Koletzko. Docosahexaenoic acid transfer into human milk after dietary supplementation: a randomized clinical trial. J. Lipid Res. 2000. 41: 1376–1383.http://www.sciencedirect.com/science/article/pii/S0022227520334490fatty acidsisotope ratio mass spectrometrylactationnutritionstable isotopes
collection DOAJ
language English
format Article
sources DOAJ
author Nataša Fidler
Thorsten Sauerwald
Anja Pohl
Hans Demmelmair
Berthold Koletzko
spellingShingle Nataša Fidler
Thorsten Sauerwald
Anja Pohl
Hans Demmelmair
Berthold Koletzko
Docosahexaenoic acid transfer into human milk after dietary supplementation: a randomized clinical trial
Journal of Lipid Research
fatty acids
isotope ratio mass spectrometry
lactation
nutrition
stable isotopes
author_facet Nataša Fidler
Thorsten Sauerwald
Anja Pohl
Hans Demmelmair
Berthold Koletzko
author_sort Nataša Fidler
title Docosahexaenoic acid transfer into human milk after dietary supplementation: a randomized clinical trial
title_short Docosahexaenoic acid transfer into human milk after dietary supplementation: a randomized clinical trial
title_full Docosahexaenoic acid transfer into human milk after dietary supplementation: a randomized clinical trial
title_fullStr Docosahexaenoic acid transfer into human milk after dietary supplementation: a randomized clinical trial
title_full_unstemmed Docosahexaenoic acid transfer into human milk after dietary supplementation: a randomized clinical trial
title_sort docosahexaenoic acid transfer into human milk after dietary supplementation: a randomized clinical trial
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 2000-09-01
description Docosahexaenoic acid (DHA) is important for infant development. The DHA transfer from maternal diet into human milk has not been investigated in detail. We studied the effects of DHA supplementation on the fatty acid composition of human milk and the secretion of dietary 13C-labeled fatty acids, including DHA, into human milk. Ten lactating women were randomized to consume, from 4 to 6 weeks postpartum, an oil rich in DHA (DHASCO™, 200 mg of DHA/day) (n = 5) or a placebo oil (n = 5). Dietary intakes were followed by 7-day protocols. On study day 14 a single dose of [U-13C]DHASCO™ was given orally, milk samples were collected over 48 h, and milk production was recorded. Milk fatty acid composition was determined by gas-liquid chromatography and isotopic enrichment was determined by gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). Milk DHA content did not differ between the supplemented and placebo group at study entry (0.29 vs. 0.28 wt%, median). After 2 weeks of supplementation the milk DHA content was almost 2-fold higher in the supplemented versus placebo group (0.37 vs. 0.21 wt%, P = 0.003). Cumulative recovery of [13C]palmitic, [13C]oleic, and [13C]docosahexaenoic acids in human milk at 48 h was similar between supplemented and placebo groups (palmitic acid 7.40 vs. 8.14%, oleic acid 9.14 vs. 9.97%, and docosahexaenoic acid 9.09 vs. 8.03% of dose, respectively). Notable lower recovery was observed for [13C]myristic acid in both the supplemented and placebo groups, 0.62 versus 0.77% of dose. Dietary DHA supplementation increases the DHA content in human milk. DHA transfer from the diet into human milk is comparable to palmitic and oleic acid transfer. —Fidler, N., T. Sauerwald, A. Pohl, H. Demmelmair, and B. Koletzko. Docosahexaenoic acid transfer into human milk after dietary supplementation: a randomized clinical trial. J. Lipid Res. 2000. 41: 1376–1383.
topic fatty acids
isotope ratio mass spectrometry
lactation
nutrition
stable isotopes
url http://www.sciencedirect.com/science/article/pii/S0022227520334490
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