Quantitative and Qualitative Changes in the Skeletal Muscle Acetylcholinesterase Activity of Oreochromis niloticus Exposed to Methylparathion

• Main Authors: Elena Catap, Glorina Pocsidio
• Format: Article
• Language: English
• Published: University of the Philippines 1994-12-01
• Series: Science Diliman
• Subjects: acetylcholinesterase; oreochromis niloticus; methylparathion
• Online Access: http://journals.upd.edu.ph/index.php/sciencediliman/article/view/274

Spectrophotometric assays and histochemical tests for acetylcholinesterase activity in the epaxial skeletal muscle of maturing Oreochromis niloticus after in-vivo exposure to 0.10 mg/L methylparathion showed significant inhibition of the enzyme by the pesticide. The assays manifested enzyme inhibition, after 48 and 96 hours of exposure, of 43.19% and 56.62%, respectively. These results were confirmed by the occurrences of decreased sites of acetylcholinesterase activity in the muscle fibers as exhibited upon performance of histochemical tests.