Use of fractional factorial design for selection of nutrients for culturing Paecilomyces variotii in eucalyptus hemicellulosic hydrolysate
A eucalyptus hemicellulose fraction was hydrolysed by treating eucalyptus wood chips with sulfuric acid. The hydrolysate was used as the substrate to grow Paecilomyces variotii IOC-3764 cultured for 72 or 96 hours. The influence of the inhibitors, nutrients and fermentation time was verified by a 28...
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Brazilian Society of Chemical Engineering
1998-09-01
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321998000300005 |
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doaj-468d93c6d2ff465d9caface3d44c8a572020-11-24T23:20:28ZengBrazilian Society of Chemical EngineeringBrazilian Journal of Chemical Engineering0104-66321678-43831998-09-0115327310.1590/S0104-66321998000300005Use of fractional factorial design for selection of nutrients for culturing Paecilomyces variotii in eucalyptus hemicellulosic hydrolysateJ.B. Almeida e SilvaU.A. LimaM.E.S. TaquedaF.G. GuaragnaA eucalyptus hemicellulose fraction was hydrolysed by treating eucalyptus wood chips with sulfuric acid. The hydrolysate was used as the substrate to grow Paecilomyces variotii IOC-3764 cultured for 72 or 96 hours. The influence of the inhibitors, nutrients and fermentation time was verified by a 28-4 and, subsequently, a 25-1 fractional factorial design. The effects of the inhibitors (acetic acid and furfural), nutrients (rice bran, urea, potassium nitrate, ammonium sulfate, magnesium sulfate and sodium phosphate) and fermentation time were investigated. The highest yield (10.59 g/L of biomass) was obtained when the microorganisms were cultivated for 72 hours in a medium composed of 30 g/L rice bran, 9.4 g/L ammonium sulfate (2 g/L nitrogen) and 2 g/L sodium phosphate.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321998000300005Fermentationexperimental designfractional factorialxyloseglucoseacetic acidfurfuralnutrients |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
J.B. Almeida e Silva U.A. Lima M.E.S. Taqueda F.G. Guaragna |
spellingShingle |
J.B. Almeida e Silva U.A. Lima M.E.S. Taqueda F.G. Guaragna Use of fractional factorial design for selection of nutrients for culturing Paecilomyces variotii in eucalyptus hemicellulosic hydrolysate Brazilian Journal of Chemical Engineering Fermentation experimental design fractional factorial xylose glucose acetic acid furfural nutrients |
author_facet |
J.B. Almeida e Silva U.A. Lima M.E.S. Taqueda F.G. Guaragna |
author_sort |
J.B. Almeida e Silva |
title |
Use of fractional factorial design for selection of nutrients for culturing Paecilomyces variotii in eucalyptus hemicellulosic hydrolysate |
title_short |
Use of fractional factorial design for selection of nutrients for culturing Paecilomyces variotii in eucalyptus hemicellulosic hydrolysate |
title_full |
Use of fractional factorial design for selection of nutrients for culturing Paecilomyces variotii in eucalyptus hemicellulosic hydrolysate |
title_fullStr |
Use of fractional factorial design for selection of nutrients for culturing Paecilomyces variotii in eucalyptus hemicellulosic hydrolysate |
title_full_unstemmed |
Use of fractional factorial design for selection of nutrients for culturing Paecilomyces variotii in eucalyptus hemicellulosic hydrolysate |
title_sort |
use of fractional factorial design for selection of nutrients for culturing paecilomyces variotii in eucalyptus hemicellulosic hydrolysate |
publisher |
Brazilian Society of Chemical Engineering |
series |
Brazilian Journal of Chemical Engineering |
issn |
0104-6632 1678-4383 |
publishDate |
1998-09-01 |
description |
A eucalyptus hemicellulose fraction was hydrolysed by treating eucalyptus wood chips with sulfuric acid. The hydrolysate was used as the substrate to grow Paecilomyces variotii IOC-3764 cultured for 72 or 96 hours. The influence of the inhibitors, nutrients and fermentation time was verified by a 28-4 and, subsequently, a 25-1 fractional factorial design. The effects of the inhibitors (acetic acid and furfural), nutrients (rice bran, urea, potassium nitrate, ammonium sulfate, magnesium sulfate and sodium phosphate) and fermentation time were investigated. The highest yield (10.59 g/L of biomass) was obtained when the microorganisms were cultivated for 72 hours in a medium composed of 30 g/L rice bran, 9.4 g/L ammonium sulfate (2 g/L nitrogen) and 2 g/L sodium phosphate. |
topic |
Fermentation experimental design fractional factorial xylose glucose acetic acid furfural nutrients |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321998000300005 |
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