Cytokine Responses in BALB/c Mice Immunized with Ovalbumin after Oral Administration of 5-Hydroxymethylfurfural

• Main Authors: Mohammad Alizadeh, Hamed Khodaei
• Format: Article
• Language: fas
• Published: Ilam University of Medical Sciences 2018-05-01
• Series: Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām
• Subjects: 5-hydroxymethylfurfural; cytokine; ovalbumin
• Online Access: http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-3312-1&slc_lang=en&sid=1

Introduction: 5-Hydroxymethylfurfural (5-HMF) is one of the most important products of the Millard reaction. Several kinds of foods and beverages produce high amount of 5-HMF. In the recent years, many profitable effects of this compound have been demonstrated. Because of the role of T helper cells cytokines in the inflammatory responses, this study sought to investigate the effects of 5-HMF on IFN-&gamma; and IL-4 levels respectively as Th1 and Th2 cytokines in BALB/c mice immunized with ovalbumin (OVA). &nbsp; Materials & methods: BALB/c mice (n=5 for each group) were grouped as follow:&nbsp; control, vehicle, and two different treatment groups. The treatment groups received daily doses of 188 and 750 mg/kg body weight, respectively. The groups were immunized with OVA on days 7 and 28 except for the control. Interleukin 4(IL-4) and interferon gamma (IFN-&gamma;) levels were measured in serum by ELISA. &nbsp; Findings: IL-4 levels were significantly suppressed in 5-HMF treatment groups (P<0.001). IFN-&gamma; levels were significantly lowered in the treatment groups (P<0.05).&nbsp; Further, no significant difference was observed in IL-4 and IFN-&gamma; levels between the treatment groups (P>0.05). &nbsp; Discussion & conclusions: 5-HMF reduced production of selected components of Th1 and Th2 cytokine responses in immunized BALB/c mice.