The genome of the stable fly, Stomoxys calcitrans, reveals potential mechanisms underlying reproduction, host interactions, and novel targets for pest control
Abstract Background The stable fly, Stomoxys calcitrans, is a major blood-feeding pest of livestock that has near worldwide distribution, causing an annual cost of over $2 billion for control and product loss in the USA alone. Control of these flies has been limited to increased sanitary management...
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BMC
2021-03-01
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| Series: | BMC Biology |
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| Online Access: | https://doi.org/10.1186/s12915-021-00975-9 |
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DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Pia U. Olafson Serap Aksoy Geoffrey M. Attardo Greta Buckmeier Xiaoting Chen Craig J. Coates Megan Davis Justin Dykema Scott J. Emrich Markus Friedrich Christopher J. Holmes Panagiotis Ioannidis Evan N. Jansen Emily C. Jennings Daniel Lawson Ellen O. Martinson Gareth L. Maslen Richard P. Meisel Terence D. Murphy Dana Nayduch David R. Nelson Kennan J. Oyen Tyler J. Raszick José M. C. Ribeiro Hugh M. Robertson Andrew J. Rosendale Timothy B. Sackton Perot Saelao Sonja L. Swiger Sing-Hoi Sze Aaron M. Tarone David B. Taylor Wesley C. Warren Robert M. Waterhouse Matthew T. Weirauch John H. Werren Richard K. Wilson Evgeny M. Zdobnov Joshua B. Benoit |
| spellingShingle |
Pia U. Olafson Serap Aksoy Geoffrey M. Attardo Greta Buckmeier Xiaoting Chen Craig J. Coates Megan Davis Justin Dykema Scott J. Emrich Markus Friedrich Christopher J. Holmes Panagiotis Ioannidis Evan N. Jansen Emily C. Jennings Daniel Lawson Ellen O. Martinson Gareth L. Maslen Richard P. Meisel Terence D. Murphy Dana Nayduch David R. Nelson Kennan J. Oyen Tyler J. Raszick José M. C. Ribeiro Hugh M. Robertson Andrew J. Rosendale Timothy B. Sackton Perot Saelao Sonja L. Swiger Sing-Hoi Sze Aaron M. Tarone David B. Taylor Wesley C. Warren Robert M. Waterhouse Matthew T. Weirauch John H. Werren Richard K. Wilson Evgeny M. Zdobnov Joshua B. Benoit The genome of the stable fly, Stomoxys calcitrans, reveals potential mechanisms underlying reproduction, host interactions, and novel targets for pest control BMC Biology Stable fly genome Muscid genomics Insect orthology Chemoreceptor genes Opsin gene duplication Metabolic detoxification genes |
| author_facet |
Pia U. Olafson Serap Aksoy Geoffrey M. Attardo Greta Buckmeier Xiaoting Chen Craig J. Coates Megan Davis Justin Dykema Scott J. Emrich Markus Friedrich Christopher J. Holmes Panagiotis Ioannidis Evan N. Jansen Emily C. Jennings Daniel Lawson Ellen O. Martinson Gareth L. Maslen Richard P. Meisel Terence D. Murphy Dana Nayduch David R. Nelson Kennan J. Oyen Tyler J. Raszick José M. C. Ribeiro Hugh M. Robertson Andrew J. Rosendale Timothy B. Sackton Perot Saelao Sonja L. Swiger Sing-Hoi Sze Aaron M. Tarone David B. Taylor Wesley C. Warren Robert M. Waterhouse Matthew T. Weirauch John H. Werren Richard K. Wilson Evgeny M. Zdobnov Joshua B. Benoit |
| author_sort |
Pia U. Olafson |
| title |
The genome of the stable fly, Stomoxys calcitrans, reveals potential mechanisms underlying reproduction, host interactions, and novel targets for pest control |
| title_short |
The genome of the stable fly, Stomoxys calcitrans, reveals potential mechanisms underlying reproduction, host interactions, and novel targets for pest control |
| title_full |
The genome of the stable fly, Stomoxys calcitrans, reveals potential mechanisms underlying reproduction, host interactions, and novel targets for pest control |
| title_fullStr |
The genome of the stable fly, Stomoxys calcitrans, reveals potential mechanisms underlying reproduction, host interactions, and novel targets for pest control |
| title_full_unstemmed |
The genome of the stable fly, Stomoxys calcitrans, reveals potential mechanisms underlying reproduction, host interactions, and novel targets for pest control |
| title_sort |
genome of the stable fly, stomoxys calcitrans, reveals potential mechanisms underlying reproduction, host interactions, and novel targets for pest control |
| publisher |
BMC |
| series |
BMC Biology |
| issn |
1741-7007 |
| publishDate |
2021-03-01 |
| description |
Abstract Background The stable fly, Stomoxys calcitrans, is a major blood-feeding pest of livestock that has near worldwide distribution, causing an annual cost of over $2 billion for control and product loss in the USA alone. Control of these flies has been limited to increased sanitary management practices and insecticide application for suppressing larval stages. Few genetic and molecular resources are available to help in developing novel methods for controlling stable flies. Results This study examines stable fly biology by utilizing a combination of high-quality genome sequencing and RNA-Seq analyses targeting multiple developmental stages and tissues. In conjunction, 1600 genes were manually curated to characterize genetic features related to stable fly reproduction, vector host interactions, host-microbe dynamics, and putative targets for control. Most notable was characterization of genes associated with reproduction and identification of expanded gene families with functional associations to vision, chemosensation, immunity, and metabolic detoxification pathways. Conclusions The combined sequencing, assembly, and curation of the male stable fly genome followed by RNA-Seq and downstream analyses provide insights necessary to understand the biology of this important pest. These resources and new data will provide the groundwork for expanding the tools available to control stable fly infestations. The close relationship of Stomoxys to other blood-feeding (horn flies and Glossina) and non-blood-feeding flies (house flies, medflies, Drosophila) will facilitate understanding of the evolutionary processes associated with development of blood feeding among the Cyclorrhapha. |
| topic |
Stable fly genome Muscid genomics Insect orthology Chemoreceptor genes Opsin gene duplication Metabolic detoxification genes |
| url |
https://doi.org/10.1186/s12915-021-00975-9 |
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doaj-4a1847c75a1b47d6b38f58fe8b200d362021-03-11T12:09:24ZengBMCBMC Biology1741-70072021-03-0119113110.1186/s12915-021-00975-9The genome of the stable fly, Stomoxys calcitrans, reveals potential mechanisms underlying reproduction, host interactions, and novel targets for pest controlPia U. Olafson0Serap Aksoy1Geoffrey M. Attardo2Greta Buckmeier3Xiaoting Chen4Craig J. Coates5Megan Davis6Justin Dykema7Scott J. Emrich8Markus Friedrich9Christopher J. Holmes10Panagiotis Ioannidis11Evan N. Jansen12Emily C. Jennings13Daniel Lawson14Ellen O. Martinson15Gareth L. Maslen16Richard P. Meisel17Terence D. Murphy18Dana Nayduch19David R. Nelson20Kennan J. Oyen21Tyler J. Raszick22José M. C. Ribeiro23Hugh M. Robertson24Andrew J. Rosendale25Timothy B. Sackton26Perot Saelao27Sonja L. Swiger28Sing-Hoi Sze29Aaron M. Tarone30David B. Taylor31Wesley C. Warren32Robert M. Waterhouse33Matthew T. Weirauch34John H. Werren35Richard K. Wilson36Evgeny M. Zdobnov37Joshua B. Benoit38Livestock Arthropod Pests Research Unit, USDA-ARSDepartment of Epidemiology of Microbial Diseases, Yale School of Public HealthDepartment of Entomology and Nematology, University of California – DavisLivestock Arthropod Pests Research Unit, USDA-ARSThe Center for Autoimmune Genomics and Etiology, Cincinnati Children’s Hospital Medical CenterDepartment of Entomology, Texas A & M UniversityLivestock Arthropod Pests Research Unit, USDA-ARSDepartment of Biological Sciences, Wayne State UniversityDepartment of Electrical Engineering & Computer Science, University of TennesseeDepartment of Biological Sciences, Wayne State UniversityDepartment of Biological Sciences, University of CincinnatiDepartment of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of BioinformaticsDepartment of Biological Sciences, University of CincinnatiDepartment of Biological Sciences, University of CincinnatiThe European Molecular Biology Laboratory, The European Bioinformatics Institute, The Wellcome Genome CampusDepartment of Entomology, University of GeorgiaThe European Molecular Biology Laboratory, The European Bioinformatics Institute, The Wellcome Genome CampusDepartment of Biology and Biochemistry, University of HoustonNational Center for Biotechnology Information, National Library of Medicine, National Institutes of HealthArthropod-borne Animal Diseases Research Unit, USDA-ARSDepartment of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science CenterDepartment of Biological Sciences, University of CincinnatiDepartment of Entomology, Texas A & M UniversitySection of Vector Biology, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious DiseasesDepartment of Entomology, University of Illinois at Urbana-ChampaignDepartment of Biology, Mount St. Joseph UniversityInformatics Group, Faculty of Arts and Sciences, Harvard UniversityLivestock Arthropod Pests Research Unit, USDA-ARSDepartment of Entomology, Texas A&M AgriLife Research and Extension CenterDepartment of Computer Science & Engineering, Department of Biochemistry & Biophysics, Texas A & M UniversityDepartment of Entomology, Texas A & M UniversityAgroecosystem Management Research Unit, USDA-ARSUniversity of Missouri, Bond Life Sciences CenterDepartment of Ecology and Evolution, University of Lausanne, and Swiss Institute of BioinformaticsCenter for Autoimmune Genomics and Etiology, Divisions of Biomedical Informatics and Developmental Biology, Cincinnati Children’s Hospital Medical CenterDepartment of Biology, University of RochesterInstitute for Genomic Medicine, Nationwide Children’s HospitalDepartment of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of BioinformaticsDepartment of Biological Sciences, University of CincinnatiAbstract Background The stable fly, Stomoxys calcitrans, is a major blood-feeding pest of livestock that has near worldwide distribution, causing an annual cost of over $2 billion for control and product loss in the USA alone. Control of these flies has been limited to increased sanitary management practices and insecticide application for suppressing larval stages. Few genetic and molecular resources are available to help in developing novel methods for controlling stable flies. Results This study examines stable fly biology by utilizing a combination of high-quality genome sequencing and RNA-Seq analyses targeting multiple developmental stages and tissues. In conjunction, 1600 genes were manually curated to characterize genetic features related to stable fly reproduction, vector host interactions, host-microbe dynamics, and putative targets for control. Most notable was characterization of genes associated with reproduction and identification of expanded gene families with functional associations to vision, chemosensation, immunity, and metabolic detoxification pathways. Conclusions The combined sequencing, assembly, and curation of the male stable fly genome followed by RNA-Seq and downstream analyses provide insights necessary to understand the biology of this important pest. These resources and new data will provide the groundwork for expanding the tools available to control stable fly infestations. The close relationship of Stomoxys to other blood-feeding (horn flies and Glossina) and non-blood-feeding flies (house flies, medflies, Drosophila) will facilitate understanding of the evolutionary processes associated with development of blood feeding among the Cyclorrhapha.https://doi.org/10.1186/s12915-021-00975-9Stable fly genomeMuscid genomicsInsect orthologyChemoreceptor genesOpsin gene duplicationMetabolic detoxification genes |