Evaluation of p16 hypermethylation in oral submucous fibrosis: A quantitative and comparative analysis in buccal cells and saliva using real-time methylation-specific polymerase chain reaction
Aims: The aim of this study was to quantitatively investigate the hypermethylation of p16 gene in buccal cells and saliva of oral submucous fibrosis (OSMF) patients using real-time quantitative methylation-specific polymerase chain reaction (PCR) and to compare the values of two methods. Subjects an...
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doaj-4a20bb8166a6450e85e7fac1ef4a38ff2020-12-02T18:40:41ZengThieme Medical and Scientific Publishers Pvt. Ltd.South Asian Journal of Cancer2278-330X2278-43062016-01-0152737910.4103/2278-330X.181645Evaluation of p16 hypermethylation in oral submucous fibrosis: A quantitative and comparative analysis in buccal cells and saliva using real-time methylation-specific polymerase chain reactionSubadra KaliyaperumalSathasivasubramanian SankarapandianAims: The aim of this study was to quantitatively investigate the hypermethylation of p16 gene in buccal cells and saliva of oral submucous fibrosis (OSMF) patients using real-time quantitative methylation-specific polymerase chain reaction (PCR) and to compare the values of two methods. Subjects and Methods: A total of 120 samples were taken from 60 subjects selected for this study, of which 30 were controls and 30 patients were clinically and histopathologically diagnosed with OSMF. In both groups, two sets of samples were collected, one directly from the buccal cells through cytobrush technique and the other through salivary rinse. We analyzed the samples for the presence of p16 hypermethylation using quantitative real-time PCR. Results: In OSMF, the hypermethylation status of p16 in buccal cells was very high (93.3%) and in salivary samples, it was partially methylated (50%). However, no hypermethylation was found in controls suggesting that significant quantity of p16 hypermethylation was present in buccal cells and saliva in OSMF. Conclusions: This study indicates that buccal cell sampling may be a better method for evaluation than the salivary samples. It signifies that hypermethylation of p16 is an important factor to be considered in epigenetic alterations of normal cells to oral precancer, i.e. OSMF.http://journal.sajc.org/article.asp?issn=2278-330X;year=2016;volume=5;issue=2;spage=73;epage=79;aulast=KaliyaperumalCpG islandsepigenetichypermethylationoral submucous fibrosisp16 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Subadra Kaliyaperumal Sathasivasubramanian Sankarapandian |
spellingShingle |
Subadra Kaliyaperumal Sathasivasubramanian Sankarapandian Evaluation of p16 hypermethylation in oral submucous fibrosis: A quantitative and comparative analysis in buccal cells and saliva using real-time methylation-specific polymerase chain reaction South Asian Journal of Cancer CpG islands epigenetic hypermethylation oral submucous fibrosis p16 |
author_facet |
Subadra Kaliyaperumal Sathasivasubramanian Sankarapandian |
author_sort |
Subadra Kaliyaperumal |
title |
Evaluation of p16 hypermethylation in oral submucous fibrosis: A quantitative and comparative analysis in buccal cells and saliva using real-time methylation-specific polymerase chain reaction |
title_short |
Evaluation of p16 hypermethylation in oral submucous fibrosis: A quantitative and comparative analysis in buccal cells and saliva using real-time methylation-specific polymerase chain reaction |
title_full |
Evaluation of p16 hypermethylation in oral submucous fibrosis: A quantitative and comparative analysis in buccal cells and saliva using real-time methylation-specific polymerase chain reaction |
title_fullStr |
Evaluation of p16 hypermethylation in oral submucous fibrosis: A quantitative and comparative analysis in buccal cells and saliva using real-time methylation-specific polymerase chain reaction |
title_full_unstemmed |
Evaluation of p16 hypermethylation in oral submucous fibrosis: A quantitative and comparative analysis in buccal cells and saliva using real-time methylation-specific polymerase chain reaction |
title_sort |
evaluation of p16 hypermethylation in oral submucous fibrosis: a quantitative and comparative analysis in buccal cells and saliva using real-time methylation-specific polymerase chain reaction |
publisher |
Thieme Medical and Scientific Publishers Pvt. Ltd. |
series |
South Asian Journal of Cancer |
issn |
2278-330X 2278-4306 |
publishDate |
2016-01-01 |
description |
Aims: The aim of this study was to quantitatively investigate the hypermethylation of p16 gene in buccal cells and saliva of oral submucous fibrosis (OSMF) patients using real-time quantitative methylation-specific polymerase chain reaction (PCR) and to compare the values of two methods. Subjects and Methods: A total of 120 samples were taken from 60 subjects selected for this study, of which 30 were controls and 30 patients were clinically and histopathologically diagnosed with OSMF. In both groups, two sets of samples were collected, one directly from the buccal cells through cytobrush technique and the other through salivary rinse. We analyzed the samples for the presence of p16 hypermethylation using quantitative real-time PCR. Results: In OSMF, the hypermethylation status of p16 in buccal cells was very high (93.3%) and in salivary samples, it was partially methylated (50%). However, no hypermethylation was found in controls suggesting that significant quantity of p16 hypermethylation was present in buccal cells and saliva in OSMF. Conclusions: This study indicates that buccal cell sampling may be a better method for evaluation than the salivary samples. It signifies that hypermethylation of p16 is an important factor to be considered in epigenetic alterations of normal cells to oral precancer, i.e. OSMF. |
topic |
CpG islands epigenetic hypermethylation oral submucous fibrosis p16 |
url |
http://journal.sajc.org/article.asp?issn=2278-330X;year=2016;volume=5;issue=2;spage=73;epage=79;aulast=Kaliyaperumal |
work_keys_str_mv |
AT subadrakaliyaperumal evaluationofp16hypermethylationinoralsubmucousfibrosisaquantitativeandcomparativeanalysisinbuccalcellsandsalivausingrealtimemethylationspecificpolymerasechainreaction AT sathasivasubramaniansankarapandian evaluationofp16hypermethylationinoralsubmucousfibrosisaquantitativeandcomparativeanalysisinbuccalcellsandsalivausingrealtimemethylationspecificpolymerasechainreaction |
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1724403296462962688 |