Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop.

Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop.

The present study mainly consists of a re-evaluation of the rate at which C12E8, a typical non-ionic detergent used for membrane studies, is able to dissociate from biological membranes, with sarcoplasmic reticulum membrane vesicles being used as an example. Utilizing a brominated derivative of C12E...

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Bibliographic Details
Main Authors: Philippe Champeil, Béatrice de Foresta, Martin Picard, Carole Gauron, Dominique Georgin, Marc le Maire, Jesper V Møller, Guillaume Lenoir, Cédric Montigny
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0222932

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https://doi.org/10.1371/journal.pone.0222932

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