An iTRAQ-Based Comparative Proteomics Analysis of the Biofilm and Planktonic States of <i>Aeromonas veronii</i> TH0426

An iTRAQ-Based Comparative Proteomics Analysis of the Biofilm and Planktonic States of <i>Aeromonas veronii</i> TH0426

<i>Aeromonas veronii</i> is a virulent fish pathogen that causes extensive economic losses in the aquaculture industry worldwide. In this study, a virulent strain of <i>A. veronii</i> TH0426 was used to establish an in vitro biofilm model. The results show that the biofilm-fo...

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Main Authors: Ying Li, Bintong Yang, Jiaxin Tian, Wuwen Sun, Guiqin Wang, Aidong Qian, Chunfeng Wang, Xiaofeng Shan, Yuanhuan Kang
Format: Article
Language:English
Published: MDPI AG 2020-02-01
Series:International Journal of Molecular Sciences
Subjects:
<i>aeromonas veronii</i>
biofilm
quantitative proteomics
itraq
Online Access:https://www.mdpi.com/1422-0067/21/4/1450
id doaj-4b1d0166c756435da127fe334fceb87a
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spelling doaj-4b1d0166c756435da127fe334fceb87a2020-11-25T00:31:11ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-02-01214145010.3390/ijms21041450ijms21041450An iTRAQ-Based Comparative Proteomics Analysis of the Biofilm and Planktonic States of <i>Aeromonas veronii</i> TH0426Ying Li0Bintong Yang1Jiaxin Tian2Wuwen Sun3Guiqin Wang4Aidong Qian5Chunfeng Wang6Xiaofeng Shan7Yuanhuan Kang8Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun 130118, China<i>Aeromonas veronii</i> is a virulent fish pathogen that causes extensive economic losses in the aquaculture industry worldwide. In this study, a virulent strain of <i>A. veronii</i> TH0426 was used to establish an in vitro biofilm model. The results show that the biofilm-forming abilities of <i>A. veronii</i> TH0426 were similar in different media, peaking under conditions of 20 &#176;C and pH 6. Further, isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics methods were used to compare the differential expression of <i>A. veronii</i> between the biofilm and planktonic cells. The results show alterations in 277 proteins, with 130 being upregulated and 147 downregulated. Pathway analysis and GO (Gene Ontology) annotations indicated that these proteins are mainly involved in metabolic pathways and the biosynthesis of secondary metabolites and antibiotics. These proteins are the main factors affecting the adaptability of <i>A. veronii</i> to its external environment. MRM (multiple reaction 27 monitoring) and qPCR (qPCR) were used to verify the differential proteins of the selected <i>A. veronii</i>. This is the first report on the biofilm and planktonic cells of <i>A. veronii</i>, thus contributing to studying the infection and pathogenesis of <i>A. veronii.</i>https://www.mdpi.com/1422-0067/21/4/1450<i>aeromonas veronii</i>biofilmquantitative proteomicsitraq
collection DOAJ
language English
format Article
sources DOAJ
author Ying Li
Bintong Yang
Jiaxin Tian
Wuwen Sun
Guiqin Wang
Aidong Qian
Chunfeng Wang
Xiaofeng Shan
Yuanhuan Kang
spellingShingle Ying Li
Bintong Yang
Jiaxin Tian
Wuwen Sun
Guiqin Wang
Aidong Qian
Chunfeng Wang
Xiaofeng Shan
Yuanhuan Kang
An iTRAQ-Based Comparative Proteomics Analysis of the Biofilm and Planktonic States of <i>Aeromonas veronii</i> TH0426
International Journal of Molecular Sciences
<i>aeromonas veronii</i>
biofilm
quantitative proteomics
itraq
author_facet Ying Li
Bintong Yang
Jiaxin Tian
Wuwen Sun
Guiqin Wang
Aidong Qian
Chunfeng Wang
Xiaofeng Shan
Yuanhuan Kang
author_sort Ying Li
title An iTRAQ-Based Comparative Proteomics Analysis of the Biofilm and Planktonic States of <i>Aeromonas veronii</i> TH0426
title_short An iTRAQ-Based Comparative Proteomics Analysis of the Biofilm and Planktonic States of <i>Aeromonas veronii</i> TH0426
title_full An iTRAQ-Based Comparative Proteomics Analysis of the Biofilm and Planktonic States of <i>Aeromonas veronii</i> TH0426
title_fullStr An iTRAQ-Based Comparative Proteomics Analysis of the Biofilm and Planktonic States of <i>Aeromonas veronii</i> TH0426
title_full_unstemmed An iTRAQ-Based Comparative Proteomics Analysis of the Biofilm and Planktonic States of <i>Aeromonas veronii</i> TH0426
title_sort itraq-based comparative proteomics analysis of the biofilm and planktonic states of <i>aeromonas veronii</i> th0426
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2020-02-01
description <i>Aeromonas veronii</i> is a virulent fish pathogen that causes extensive economic losses in the aquaculture industry worldwide. In this study, a virulent strain of <i>A. veronii</i> TH0426 was used to establish an in vitro biofilm model. The results show that the biofilm-forming abilities of <i>A. veronii</i> TH0426 were similar in different media, peaking under conditions of 20 &#176;C and pH 6. Further, isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics methods were used to compare the differential expression of <i>A. veronii</i> between the biofilm and planktonic cells. The results show alterations in 277 proteins, with 130 being upregulated and 147 downregulated. Pathway analysis and GO (Gene Ontology) annotations indicated that these proteins are mainly involved in metabolic pathways and the biosynthesis of secondary metabolites and antibiotics. These proteins are the main factors affecting the adaptability of <i>A. veronii</i> to its external environment. MRM (multiple reaction 27 monitoring) and qPCR (qPCR) were used to verify the differential proteins of the selected <i>A. veronii</i>. This is the first report on the biofilm and planktonic cells of <i>A. veronii</i>, thus contributing to studying the infection and pathogenesis of <i>A. veronii.</i>
topic <i>aeromonas veronii</i>
biofilm
quantitative proteomics
itraq
url https://www.mdpi.com/1422-0067/21/4/1450
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