Intravital observation of Plasmodium berghei sporozoite infection of the liver.

Plasmodium sporozoite invasion of liver cells has been an extremely elusive event to study. In the prevailing model, sporozoites enter the liver by passing through Kupffer cells, but this model was based solely on incidental observations in fixed specimens and on biochemical and physiological data....

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Main Authors: Ute Frevert, Sabine Engelmann, Sergine Zougbédé, Jörg Stange, Bruce Ng, Kai Matuschewski, Leonard Liebes, Herman Yee
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2005-06-01
Series:PLoS Biology
Online Access:http://europepmc.org/articles/PMC1135295?pdf=render
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spelling doaj-4c44ca39fc3e405a80a2de4aeb57510f2021-07-02T13:48:05ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852005-06-0136e19210.1371/journal.pbio.0030192Intravital observation of Plasmodium berghei sporozoite infection of the liver.Ute FrevertSabine EngelmannSergine ZougbédéJörg StangeBruce NgKai MatuschewskiLeonard LiebesHerman YeePlasmodium sporozoite invasion of liver cells has been an extremely elusive event to study. In the prevailing model, sporozoites enter the liver by passing through Kupffer cells, but this model was based solely on incidental observations in fixed specimens and on biochemical and physiological data. To obtain direct information on the dynamics of sporozoite infection of the liver, we infected live mice with red or green fluorescent Plasmodium berghei sporozoites and monitored their behavior using intravital microscopy. Digital recordings show that sporozoites entering a liver lobule abruptly adhere to the sinusoidal cell layer, suggesting a high-affinity interaction. They glide along the sinusoid, with or against the bloodstream, to a Kupffer cell, and, by slowly pushing through a constriction, traverse across the space of Disse. Once inside the liver parenchyma, sporozoites move rapidly for many minutes, traversing several hepatocytes, until ultimately settling within a final one. Migration damage to hepatocytes was confirmed in liver sections, revealing clusters of necrotic hepatocytes adjacent to structurally intact, sporozoite-infected hepatocytes, and by elevated serum alanine aminotransferase activity. In summary, malaria sporozoites bind tightly to the sinusoidal cell layer, cross Kupffer cells, and leave behind a trail of dead hepatocytes when migrating to their final destination in the liver.http://europepmc.org/articles/PMC1135295?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Ute Frevert
Sabine Engelmann
Sergine Zougbédé
Jörg Stange
Bruce Ng
Kai Matuschewski
Leonard Liebes
Herman Yee
spellingShingle Ute Frevert
Sabine Engelmann
Sergine Zougbédé
Jörg Stange
Bruce Ng
Kai Matuschewski
Leonard Liebes
Herman Yee
Intravital observation of Plasmodium berghei sporozoite infection of the liver.
PLoS Biology
author_facet Ute Frevert
Sabine Engelmann
Sergine Zougbédé
Jörg Stange
Bruce Ng
Kai Matuschewski
Leonard Liebes
Herman Yee
author_sort Ute Frevert
title Intravital observation of Plasmodium berghei sporozoite infection of the liver.
title_short Intravital observation of Plasmodium berghei sporozoite infection of the liver.
title_full Intravital observation of Plasmodium berghei sporozoite infection of the liver.
title_fullStr Intravital observation of Plasmodium berghei sporozoite infection of the liver.
title_full_unstemmed Intravital observation of Plasmodium berghei sporozoite infection of the liver.
title_sort intravital observation of plasmodium berghei sporozoite infection of the liver.
publisher Public Library of Science (PLoS)
series PLoS Biology
issn 1544-9173
1545-7885
publishDate 2005-06-01
description Plasmodium sporozoite invasion of liver cells has been an extremely elusive event to study. In the prevailing model, sporozoites enter the liver by passing through Kupffer cells, but this model was based solely on incidental observations in fixed specimens and on biochemical and physiological data. To obtain direct information on the dynamics of sporozoite infection of the liver, we infected live mice with red or green fluorescent Plasmodium berghei sporozoites and monitored their behavior using intravital microscopy. Digital recordings show that sporozoites entering a liver lobule abruptly adhere to the sinusoidal cell layer, suggesting a high-affinity interaction. They glide along the sinusoid, with or against the bloodstream, to a Kupffer cell, and, by slowly pushing through a constriction, traverse across the space of Disse. Once inside the liver parenchyma, sporozoites move rapidly for many minutes, traversing several hepatocytes, until ultimately settling within a final one. Migration damage to hepatocytes was confirmed in liver sections, revealing clusters of necrotic hepatocytes adjacent to structurally intact, sporozoite-infected hepatocytes, and by elevated serum alanine aminotransferase activity. In summary, malaria sporozoites bind tightly to the sinusoidal cell layer, cross Kupffer cells, and leave behind a trail of dead hepatocytes when migrating to their final destination in the liver.
url http://europepmc.org/articles/PMC1135295?pdf=render
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