“Candidatus Neoehrlichia mikurensis” in Ixodes ricinus ticks collected near the Arctic Circle in Norway
Abstract Background “Candidatus Neoehrlichia mikurensis” is a gram-negative bacterium belonging to the family Anaplasmataceae that, in Europe, is transmitted by Ixodes ricinus ticks. “Candidatus N. mikurensis” can cause a severe systemic inflammatory syndrome, neoehrlichiosis, mostly in persons with...
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doaj-4db7c9ccd769446e960cd10f51fc4f572020-11-25T02:31:46ZengBMCParasites & Vectors1756-33052018-12-011111810.1186/s13071-018-3168-y“Candidatus Neoehrlichia mikurensis” in Ixodes ricinus ticks collected near the Arctic Circle in NorwayClarinda Larsson0Dag Hvidsten1Snorre Stuen2Anna J. Henningsson3Peter Wilhelmsson4Faculty of Medicine and Health Sciences, Linköping UniversityDepartment of Microbiology and Infection Control, University Hospital of North NorwayDepartment of Production Animal Clinical Sciences, Section of Small Ruminant Research and Herd Health, Norwegian University of Life SciencesClinical Microbiology, Laboratory Medicine, County Hospital RyhovDivision of Medical Microbiology, Department of Clinical and Experimental Medicine, Linköping UniversityAbstract Background “Candidatus Neoehrlichia mikurensis” is a gram-negative bacterium belonging to the family Anaplasmataceae that, in Europe, is transmitted by Ixodes ricinus ticks. “Candidatus N. mikurensis” can cause a severe systemic inflammatory syndrome, neoehrlichiosis, mostly in persons with other underlying diseases. To date, “Ca. N. mikurensis” has been found in ticks in different countries in Asia and Europe, but never as far north as at the Arctic Circle. Methods A total of 1104 I. ricinus ticks collected from vegetation and from animals in northern Norway (64–68°N) were analysed for the prevalence of “Ca. N. mikurensis”. Of them, 495 ticks were collected from vegetation by flagging and 609 ticks were collected from dogs and cats. Total nucleic acid extracted from the ticks were converted to cDNA and analyzed with real-time PCR targeting the 16S rRNA gene of “Ca. N. mikurensis”. Positive samples were further analysed by nested PCR and sequencing. Results “Candidatus N. mikurensis” was detected in 11.2% of all collected I. ricinus ticks in northern Norway. The prevalence differed between ticks collected from vegetation (18.2%; 90/495) compared to ticks collected from dogs and cats (5.6%; 34/609). The ticks from dogs and cats were collected in Brønnøy area and seven additional districts further north. The prevalence of “Ca. N. mikurensis” in these ticks differed between geographical localities, with the highest prevalence in the Brønnøy area. Conclusions The detection of “Ca. N. mikurensis” in I. ricinus ticks from the Arctic Circle in northern Norway indicates potential risk for tick-bitten humans at this latitude to be infected with “Ca. N. mikurensis”.http://link.springer.com/article/10.1186/s13071-018-3168-y“Candidatus Neoehrlichia mikurensis”Ixodes ricinusNeoehrlichiosisTick-borne pathogenArctic CircleNorway |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Clarinda Larsson Dag Hvidsten Snorre Stuen Anna J. Henningsson Peter Wilhelmsson |
spellingShingle |
Clarinda Larsson Dag Hvidsten Snorre Stuen Anna J. Henningsson Peter Wilhelmsson “Candidatus Neoehrlichia mikurensis” in Ixodes ricinus ticks collected near the Arctic Circle in Norway Parasites & Vectors “Candidatus Neoehrlichia mikurensis” Ixodes ricinus Neoehrlichiosis Tick-borne pathogen Arctic Circle Norway |
author_facet |
Clarinda Larsson Dag Hvidsten Snorre Stuen Anna J. Henningsson Peter Wilhelmsson |
author_sort |
Clarinda Larsson |
title |
“Candidatus Neoehrlichia mikurensis” in Ixodes ricinus ticks collected near the Arctic Circle in Norway |
title_short |
“Candidatus Neoehrlichia mikurensis” in Ixodes ricinus ticks collected near the Arctic Circle in Norway |
title_full |
“Candidatus Neoehrlichia mikurensis” in Ixodes ricinus ticks collected near the Arctic Circle in Norway |
title_fullStr |
“Candidatus Neoehrlichia mikurensis” in Ixodes ricinus ticks collected near the Arctic Circle in Norway |
title_full_unstemmed |
“Candidatus Neoehrlichia mikurensis” in Ixodes ricinus ticks collected near the Arctic Circle in Norway |
title_sort |
“candidatus neoehrlichia mikurensis” in ixodes ricinus ticks collected near the arctic circle in norway |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2018-12-01 |
description |
Abstract Background “Candidatus Neoehrlichia mikurensis” is a gram-negative bacterium belonging to the family Anaplasmataceae that, in Europe, is transmitted by Ixodes ricinus ticks. “Candidatus N. mikurensis” can cause a severe systemic inflammatory syndrome, neoehrlichiosis, mostly in persons with other underlying diseases. To date, “Ca. N. mikurensis” has been found in ticks in different countries in Asia and Europe, but never as far north as at the Arctic Circle. Methods A total of 1104 I. ricinus ticks collected from vegetation and from animals in northern Norway (64–68°N) were analysed for the prevalence of “Ca. N. mikurensis”. Of them, 495 ticks were collected from vegetation by flagging and 609 ticks were collected from dogs and cats. Total nucleic acid extracted from the ticks were converted to cDNA and analyzed with real-time PCR targeting the 16S rRNA gene of “Ca. N. mikurensis”. Positive samples were further analysed by nested PCR and sequencing. Results “Candidatus N. mikurensis” was detected in 11.2% of all collected I. ricinus ticks in northern Norway. The prevalence differed between ticks collected from vegetation (18.2%; 90/495) compared to ticks collected from dogs and cats (5.6%; 34/609). The ticks from dogs and cats were collected in Brønnøy area and seven additional districts further north. The prevalence of “Ca. N. mikurensis” in these ticks differed between geographical localities, with the highest prevalence in the Brønnøy area. Conclusions The detection of “Ca. N. mikurensis” in I. ricinus ticks from the Arctic Circle in northern Norway indicates potential risk for tick-bitten humans at this latitude to be infected with “Ca. N. mikurensis”. |
topic |
“Candidatus Neoehrlichia mikurensis” Ixodes ricinus Neoehrlichiosis Tick-borne pathogen Arctic Circle Norway |
url |
http://link.springer.com/article/10.1186/s13071-018-3168-y |
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