Energy-Efficient Single-Stage Nitrite Shunt Denitrification with Saline Sewage through Concise Dissolved Oxygen (DO) Supply: Process Performance and Microbial Communities
Single-stage nitrite shunt denitrification (through nitrite rather than nitrate) with low dissolved oxygen (DO) supply is a better alternative in terms of energy-efficiency, short-footprint, and low C/N-ratio requirement. This study investigates the optimal DO level with temperature effect, with sal...
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doaj-4edc03a66f734cb8bc11c2011fccd0e82020-11-25T03:12:06ZengMDPI AGMicroorganisms2076-26072020-06-01891991910.3390/microorganisms8060919Energy-Efficient Single-Stage Nitrite Shunt Denitrification with Saline Sewage through Concise Dissolved Oxygen (DO) Supply: Process Performance and Microbial CommunitiesHuichuan Zhuang0Zhuoying Wu1Linji Xu2Shao-Yuan Leu3Po-Heng Lee4Department of Civil and Environmental Engineering, Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, ChinaDepartment of Civil and Environmental Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UKEnvironmental Engineering Technology Research Center, Chongqing Academy of Ecology and Environmental Sciences, Chongqing 401147, ChinaDepartment of Civil and Environmental Engineering, Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, ChinaDepartment of Civil and Environmental Engineering, Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, ChinaSingle-stage nitrite shunt denitrification (through nitrite rather than nitrate) with low dissolved oxygen (DO) supply is a better alternative in terms of energy-efficiency, short-footprint, and low C/N-ratio requirement. This study investigates the optimal DO level with temperature effect, with saline sewage at the fixed hydraulic and solids retention times of 8 h and 8 d, respectively. Moreover, 16S rRNA gene sequencing analysis corresponding with total nitrogen (TN) and chemical oxygen demand (COD) removals in each operating condition were performed. Results showed that DO of 0.3 mg/L at 20 °C achieved over 60.7% and over 97.9% of TN and COD removal, respectively, suggesting that such condition achieved effective nitrite-oxidizing bacteria inhibition and efficient denitrification. An unexpected finding was that sulfur-reducing <i>Haematobacter</i> and nitrogen-fixing <i>Geofilum</i> and <i>Shinella</i> were highly abundant with the copredominance of ammonia-oxidizing <i>Comamonas</i> and <i>Nitrosomonas</i>, nitrite-oxidizing <i>Limnohabitans</i>, and denitrifying <i>Simplicispira</i>, <i>Castellaniella,</i> and <i>Nitratireductor</i>. Further, canonical correspondence analysis (CCA) with respect to the operating conditions associated with phenotype prediction via R-based tool Tax4Fun was performed for a preliminary diagnosis of microbial functionality. The effects of DO, temperature, nitrite, and nitrate in various extents toward each predominant microbe were discussed. Collectively, DO is likely pivotal in single-stage nitrite shunt denitrification, as well as microbial communities, for energy-efficient saline sewage treatment.https://www.mdpi.com/2076-2607/8/6/919nitrite shunt denitrification16S rRNA sequencingdissolve oxygentotal nitrogen removal |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huichuan Zhuang Zhuoying Wu Linji Xu Shao-Yuan Leu Po-Heng Lee |
spellingShingle |
Huichuan Zhuang Zhuoying Wu Linji Xu Shao-Yuan Leu Po-Heng Lee Energy-Efficient Single-Stage Nitrite Shunt Denitrification with Saline Sewage through Concise Dissolved Oxygen (DO) Supply: Process Performance and Microbial Communities Microorganisms nitrite shunt denitrification 16S rRNA sequencing dissolve oxygen total nitrogen removal |
author_facet |
Huichuan Zhuang Zhuoying Wu Linji Xu Shao-Yuan Leu Po-Heng Lee |
author_sort |
Huichuan Zhuang |
title |
Energy-Efficient Single-Stage Nitrite Shunt Denitrification with Saline Sewage through Concise Dissolved Oxygen (DO) Supply: Process Performance and Microbial Communities |
title_short |
Energy-Efficient Single-Stage Nitrite Shunt Denitrification with Saline Sewage through Concise Dissolved Oxygen (DO) Supply: Process Performance and Microbial Communities |
title_full |
Energy-Efficient Single-Stage Nitrite Shunt Denitrification with Saline Sewage through Concise Dissolved Oxygen (DO) Supply: Process Performance and Microbial Communities |
title_fullStr |
Energy-Efficient Single-Stage Nitrite Shunt Denitrification with Saline Sewage through Concise Dissolved Oxygen (DO) Supply: Process Performance and Microbial Communities |
title_full_unstemmed |
Energy-Efficient Single-Stage Nitrite Shunt Denitrification with Saline Sewage through Concise Dissolved Oxygen (DO) Supply: Process Performance and Microbial Communities |
title_sort |
energy-efficient single-stage nitrite shunt denitrification with saline sewage through concise dissolved oxygen (do) supply: process performance and microbial communities |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2020-06-01 |
description |
Single-stage nitrite shunt denitrification (through nitrite rather than nitrate) with low dissolved oxygen (DO) supply is a better alternative in terms of energy-efficiency, short-footprint, and low C/N-ratio requirement. This study investigates the optimal DO level with temperature effect, with saline sewage at the fixed hydraulic and solids retention times of 8 h and 8 d, respectively. Moreover, 16S rRNA gene sequencing analysis corresponding with total nitrogen (TN) and chemical oxygen demand (COD) removals in each operating condition were performed. Results showed that DO of 0.3 mg/L at 20 °C achieved over 60.7% and over 97.9% of TN and COD removal, respectively, suggesting that such condition achieved effective nitrite-oxidizing bacteria inhibition and efficient denitrification. An unexpected finding was that sulfur-reducing <i>Haematobacter</i> and nitrogen-fixing <i>Geofilum</i> and <i>Shinella</i> were highly abundant with the copredominance of ammonia-oxidizing <i>Comamonas</i> and <i>Nitrosomonas</i>, nitrite-oxidizing <i>Limnohabitans</i>, and denitrifying <i>Simplicispira</i>, <i>Castellaniella,</i> and <i>Nitratireductor</i>. Further, canonical correspondence analysis (CCA) with respect to the operating conditions associated with phenotype prediction via R-based tool Tax4Fun was performed for a preliminary diagnosis of microbial functionality. The effects of DO, temperature, nitrite, and nitrate in various extents toward each predominant microbe were discussed. Collectively, DO is likely pivotal in single-stage nitrite shunt denitrification, as well as microbial communities, for energy-efficient saline sewage treatment. |
topic |
nitrite shunt denitrification 16S rRNA sequencing dissolve oxygen total nitrogen removal |
url |
https://www.mdpi.com/2076-2607/8/6/919 |
work_keys_str_mv |
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