<i>Solidago virgaurea</i> L. Plant Extract Targeted Against <i>Candida albicans</i> to Reduce Oral Microbial Biomass: a Double Blind Randomized Trial on Healthy Adults

Oral microbiome plays an important part on oral health and endogenous bacteria and fungi should not be eradicated. However, their proliferation must be controlled by oral hygiene care. In vitro, <i>Solidago virgaurea</i> ssp. <i>virgaurea</i> L. (SV) plant extract inhibits th...

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Main Authors: Isabelle Prêcheur, Yohan Rolland, Lilia Hasseine, François Orange, Adeline Morisot, Anne Landreau
Format: Article
Language:English
Published: MDPI AG 2020-03-01
Series:Antibiotics
Subjects:
Online Access:https://www.mdpi.com/2079-6382/9/4/137
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spelling doaj-4f6275e6226e4a169fdcb6a5f71ec39c2020-11-25T01:54:15ZengMDPI AGAntibiotics2079-63822020-03-019413710.3390/antibiotics9040137antibiotics9040137<i>Solidago virgaurea</i> L. Plant Extract Targeted Against <i>Candida albicans</i> to Reduce Oral Microbial Biomass: a Double Blind Randomized Trial on Healthy AdultsIsabelle Prêcheur0Yohan Rolland1Lilia Hasseine2François Orange3Adeline Morisot4Anne Landreau5Université Nice Côte d’Azur, Faculté de Chirurgie Dentaire, Laboratoire Micoralis EA 7354, 06300 Nice, FranceGivaudan, Naturex, 84140 Avignon, FranceUniversité Nice Côte d’Azur, Centre Hospitalier Universitaire, Laboratoire de Parasitologie-Mycologie, 06200 Nice, FranceUniversité Nice Côte d’Azur, Faculté des Sciences, Centre Commun de Microscopie Appliquée (CCMA), 06000 Nice, FranceUniversité Nice Côte d’Azur, Centre Hospitalier Universitaire, Département de Santé Publique, 06200 Nice, FranceUniversité Nice Côte d’Azur, Centre Hospitalier Universitaire, Laboratoire de Parasitologie-Mycologie, 06200 Nice, FranceOral microbiome plays an important part on oral health and endogenous bacteria and fungi should not be eradicated. However, their proliferation must be controlled by oral hygiene care. In vitro, <i>Solidago virgaurea</i> ssp. <i>virgaurea</i> L. (SV) plant extract inhibits the adherence and hyphal formation of a fungus, <i>Candida albicans</i>. It reduces the biomass of <i>Candida</i>-bacterial biofilms but not fungal or bacterial growth. Unlike chemical antiseptics, like triclosan and chlorhexidine for instance, SV is a plant extract easily biodegradable. The purpose of this study was to assess the in vivo effectiveness of SV extract in reducing oral biomass. A randomized, double-blind clinical study, with dental plaque evaluation designed to assess the effectiveness of a fluorinated toothpaste containing SV (Bucovia&#8482;, Givaudan, Vernier, Switzerland) was conducted. Sixty-six subjects (SV group <i>n</i> = 33 vs. control <i>n</i> = 33) brushed their teeth twice a day for a 4-week period. Supragingival dental plaque was sampled. Total bacterial load (broad spectral bacterial quantitative Polymerase Chain Reaction (qPCR)), <i>C. albicans</i> and seven bacterial species were quantified by qPCR. In the Intervention group, there was a decrease of Total bacterial load (&#916;D0D28 <i>p</i> = 0.005 and &#916;D14D28 <i>p</i> = 0.026), <i>Streptococcus mutans</i> (&#916;D0D14 <i>p </i>= 0.024) and <i>C. albicans</i> (&#916;D0D28 <i>p</i> = 0.022). In the Control group Total bacterial load tended to decrease from baseline to day 28 (&#916;D0D28 <i>p</i> = 0.062 and &#916;D14D28 <i>p</i> = 0.009). Plaque Index and Gingival Index improved in both groups.https://www.mdpi.com/2079-6382/9/4/137antisepticsbiodegradablebiofilmcandida albicansdental plaqueoral care productsmycobiomesolidago virgaurea
collection DOAJ
language English
format Article
sources DOAJ
author Isabelle Prêcheur
Yohan Rolland
Lilia Hasseine
François Orange
Adeline Morisot
Anne Landreau
spellingShingle Isabelle Prêcheur
Yohan Rolland
Lilia Hasseine
François Orange
Adeline Morisot
Anne Landreau
<i>Solidago virgaurea</i> L. Plant Extract Targeted Against <i>Candida albicans</i> to Reduce Oral Microbial Biomass: a Double Blind Randomized Trial on Healthy Adults
Antibiotics
antiseptics
biodegradable
biofilm
candida albicans
dental plaque
oral care products
mycobiome
solidago virgaurea
author_facet Isabelle Prêcheur
Yohan Rolland
Lilia Hasseine
François Orange
Adeline Morisot
Anne Landreau
author_sort Isabelle Prêcheur
title <i>Solidago virgaurea</i> L. Plant Extract Targeted Against <i>Candida albicans</i> to Reduce Oral Microbial Biomass: a Double Blind Randomized Trial on Healthy Adults
title_short <i>Solidago virgaurea</i> L. Plant Extract Targeted Against <i>Candida albicans</i> to Reduce Oral Microbial Biomass: a Double Blind Randomized Trial on Healthy Adults
title_full <i>Solidago virgaurea</i> L. Plant Extract Targeted Against <i>Candida albicans</i> to Reduce Oral Microbial Biomass: a Double Blind Randomized Trial on Healthy Adults
title_fullStr <i>Solidago virgaurea</i> L. Plant Extract Targeted Against <i>Candida albicans</i> to Reduce Oral Microbial Biomass: a Double Blind Randomized Trial on Healthy Adults
title_full_unstemmed <i>Solidago virgaurea</i> L. Plant Extract Targeted Against <i>Candida albicans</i> to Reduce Oral Microbial Biomass: a Double Blind Randomized Trial on Healthy Adults
title_sort <i>solidago virgaurea</i> l. plant extract targeted against <i>candida albicans</i> to reduce oral microbial biomass: a double blind randomized trial on healthy adults
publisher MDPI AG
series Antibiotics
issn 2079-6382
publishDate 2020-03-01
description Oral microbiome plays an important part on oral health and endogenous bacteria and fungi should not be eradicated. However, their proliferation must be controlled by oral hygiene care. In vitro, <i>Solidago virgaurea</i> ssp. <i>virgaurea</i> L. (SV) plant extract inhibits the adherence and hyphal formation of a fungus, <i>Candida albicans</i>. It reduces the biomass of <i>Candida</i>-bacterial biofilms but not fungal or bacterial growth. Unlike chemical antiseptics, like triclosan and chlorhexidine for instance, SV is a plant extract easily biodegradable. The purpose of this study was to assess the in vivo effectiveness of SV extract in reducing oral biomass. A randomized, double-blind clinical study, with dental plaque evaluation designed to assess the effectiveness of a fluorinated toothpaste containing SV (Bucovia&#8482;, Givaudan, Vernier, Switzerland) was conducted. Sixty-six subjects (SV group <i>n</i> = 33 vs. control <i>n</i> = 33) brushed their teeth twice a day for a 4-week period. Supragingival dental plaque was sampled. Total bacterial load (broad spectral bacterial quantitative Polymerase Chain Reaction (qPCR)), <i>C. albicans</i> and seven bacterial species were quantified by qPCR. In the Intervention group, there was a decrease of Total bacterial load (&#916;D0D28 <i>p</i> = 0.005 and &#916;D14D28 <i>p</i> = 0.026), <i>Streptococcus mutans</i> (&#916;D0D14 <i>p </i>= 0.024) and <i>C. albicans</i> (&#916;D0D28 <i>p</i> = 0.022). In the Control group Total bacterial load tended to decrease from baseline to day 28 (&#916;D0D28 <i>p</i> = 0.062 and &#916;D14D28 <i>p</i> = 0.009). Plaque Index and Gingival Index improved in both groups.
topic antiseptics
biodegradable
biofilm
candida albicans
dental plaque
oral care products
mycobiome
solidago virgaurea
url https://www.mdpi.com/2079-6382/9/4/137
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