Random and combinatorial mutagenesis for improved total production of secretory target protein in Escherichia coli

Abstract Signal peptides and secretory carrier proteins are commonly used to secrete heterologous recombinant protein in Gram-negative bacteria. The Escherichia coli osmotically-inducible protein Y (OsmY) is a carrier protein that secretes a target protein extracellularly, and we have previously app...

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Bibliographic Details
Main Authors: David Gonzalez-Perez, James Ratcliffe, Shu Khan Tan, Mary Chen May Wong, Yi Pei Yee, Natsai Nyabadza, Jian-He Xu, Tuck Seng Wong, Kang Lan Tee
Format: Article
Language:English
Published: Nature Publishing Group 2021-03-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-84859-6