Evaluation of the Phytochemical, Antioxidant, Enzyme Inhibition, and Wound Healing Potential of Calotropis gigantea (L.) Dryand: A Source of a Bioactive Medicinal Product

Traditionally, plants of the genus Calotropis have been used to cure various common diseases. The present research work explores the chemical and biological characterization of one of the most common species of this genus, i.e., Calotropis gigantea (L.) Dryand (syn. Calotropis gigantea (L.) Dryand.)...

Full description

Bibliographic Details
Main Authors: Ahmed Alafnan, Swathi Sridharagatta, Hammad Saleem, Umair Khurshid, Abdulwahab Alamri, Shabana Yasmeen Ansari, Syafiq Asnawi Zainal Abidin, Siddique Akber Ansari, Abdulhakeem S. Alamri, Nafees Ahemad, Sirajudheen Anwar
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-08-01
Series:Frontiers in Pharmacology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2021.701369/full
id doaj-52eae9e885834a188675e821ee0c48df
record_format Article
spelling doaj-52eae9e885834a188675e821ee0c48df2021-08-17T11:31:40ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-08-011210.3389/fphar.2021.701369701369Evaluation of the Phytochemical, Antioxidant, Enzyme Inhibition, and Wound Healing Potential of Calotropis gigantea (L.) Dryand: A Source of a Bioactive Medicinal ProductAhmed Alafnan0Swathi Sridharagatta1Hammad Saleem2Hammad Saleem3Umair Khurshid4Abdulwahab Alamri5Shabana Yasmeen Ansari6Syafiq Asnawi Zainal Abidin7Siddique Akber Ansari8Abdulhakeem S. Alamri9Nafees Ahemad10Sirajudheen Anwar11Department of Pharmacology and Toxicology, College of Pharmacy, University of Hail, Hail, Saudi ArabiaDepartment of Pharmacognosy, Vydehi Institute of Pharmacy, Bangalore, IndiaInstitute of Pharmaceutical Sciences (IPS), University of Veterinary and Animal Sciences (UVAS), Lahore, PakistanSchool of Pharmacy, Monash University Malaysia, Darul Ehsan, MalaysiaBahawalpur College of Pharmacy, Bahawalpur Medical and Dental College, Bahawalpur, PakistanDepartment of Pharmacology and Toxicology, College of Pharmacy, University of Hail, Hail, Saudi ArabiaDepartment of Chemical and Electronic Engineering, Pharmaceutical Unit, University of Messina, Messina, ItalyLiquid Chromatography Mass Spectrometry (LCMS) Platform, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Selangor Darul Ehsan, MalaysiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Science, Taif University, Taif, Saudi ArabiaSchool of Pharmacy, Monash University Malaysia, Darul Ehsan, MalaysiaDepartment of Pharmacology and Toxicology, College of Pharmacy, University of Hail, Hail, Saudi ArabiaTraditionally, plants of the genus Calotropis have been used to cure various common diseases. The present research work explores the chemical and biological characterization of one of the most common species of this genus, i.e., Calotropis gigantea (L.) Dryand (syn. Calotropis gigantea (L.) Dryand.), having multiple folklore applications. The ethanolic extract of leaves of Calotropis gigantea (L.) Dryand was analyzed for the phytochemical composition by determining the total bioactive (total phenolic and total flavonoid) contents and UHPLC-MS secondary metabolites analysis. For phytopharmacological evaluation, in vitro antioxidant (including DPPH, ABTS, FRAP, CUPRAC, phosphomolybdenum, and metal chelation antioxidant assays) activities, enzyme inhibition potential (against AChE, BChE, α-amylase, and tyrosinase enzymes), and in vivo wound healing potential were determined. The tested extract has been shown to contain considerable flavonoid (46.75 mg RE/g extract) and phenolic (33.71 mg GAE/g extract) contents. The plant extract presented considerable antioxidant potential, being the most active for CUPRAC assays. Secondary metabolite UHPLC-MS characterization, in both the positive and negative ionization modes, indicated the tentative presence of 17 different phytocompounds, mostly derivatives of sesquiterpene, alkaloids, and flavonoids. Similarly, the tested extract exhibited considerable inhibitory effects on tyrosinase (81.72 mg KAE/g extract), whereas it showed weak inhibition ability against other tested enzymes. Moreover, in the case of in vivo wound healing assays, significant improvement in wound healing was observed in both the tested models at the doses of 0.5 percent w/w (p < 0.001) and 2.0 percent w/w (p < 0.01) on the 16th day. The outcomes of the present research work suggested that C. gigantea (L.) Dryand plant extract could be appraised as a potential origin of bioactive molecules having multifunctional medicinal uses.https://www.frontiersin.org/articles/10.3389/fphar.2021.701369/fullCalotropis giganteaphytochemicalsphenolic contentantioxidantenzyme inhibitionwound healing
collection DOAJ
language English
format Article
sources DOAJ
author Ahmed Alafnan
Swathi Sridharagatta
Hammad Saleem
Hammad Saleem
Umair Khurshid
Abdulwahab Alamri
Shabana Yasmeen Ansari
Syafiq Asnawi Zainal Abidin
Siddique Akber Ansari
Abdulhakeem S. Alamri
Nafees Ahemad
Sirajudheen Anwar
spellingShingle Ahmed Alafnan
Swathi Sridharagatta
Hammad Saleem
Hammad Saleem
Umair Khurshid
Abdulwahab Alamri
Shabana Yasmeen Ansari
Syafiq Asnawi Zainal Abidin
Siddique Akber Ansari
Abdulhakeem S. Alamri
Nafees Ahemad
Sirajudheen Anwar
Evaluation of the Phytochemical, Antioxidant, Enzyme Inhibition, and Wound Healing Potential of Calotropis gigantea (L.) Dryand: A Source of a Bioactive Medicinal Product
Frontiers in Pharmacology
Calotropis gigantea
phytochemicals
phenolic content
antioxidant
enzyme inhibition
wound healing
author_facet Ahmed Alafnan
Swathi Sridharagatta
Hammad Saleem
Hammad Saleem
Umair Khurshid
Abdulwahab Alamri
Shabana Yasmeen Ansari
Syafiq Asnawi Zainal Abidin
Siddique Akber Ansari
Abdulhakeem S. Alamri
Nafees Ahemad
Sirajudheen Anwar
author_sort Ahmed Alafnan
title Evaluation of the Phytochemical, Antioxidant, Enzyme Inhibition, and Wound Healing Potential of Calotropis gigantea (L.) Dryand: A Source of a Bioactive Medicinal Product
title_short Evaluation of the Phytochemical, Antioxidant, Enzyme Inhibition, and Wound Healing Potential of Calotropis gigantea (L.) Dryand: A Source of a Bioactive Medicinal Product
title_full Evaluation of the Phytochemical, Antioxidant, Enzyme Inhibition, and Wound Healing Potential of Calotropis gigantea (L.) Dryand: A Source of a Bioactive Medicinal Product
title_fullStr Evaluation of the Phytochemical, Antioxidant, Enzyme Inhibition, and Wound Healing Potential of Calotropis gigantea (L.) Dryand: A Source of a Bioactive Medicinal Product
title_full_unstemmed Evaluation of the Phytochemical, Antioxidant, Enzyme Inhibition, and Wound Healing Potential of Calotropis gigantea (L.) Dryand: A Source of a Bioactive Medicinal Product
title_sort evaluation of the phytochemical, antioxidant, enzyme inhibition, and wound healing potential of calotropis gigantea (l.) dryand: a source of a bioactive medicinal product
publisher Frontiers Media S.A.
series Frontiers in Pharmacology
issn 1663-9812
publishDate 2021-08-01
description Traditionally, plants of the genus Calotropis have been used to cure various common diseases. The present research work explores the chemical and biological characterization of one of the most common species of this genus, i.e., Calotropis gigantea (L.) Dryand (syn. Calotropis gigantea (L.) Dryand.), having multiple folklore applications. The ethanolic extract of leaves of Calotropis gigantea (L.) Dryand was analyzed for the phytochemical composition by determining the total bioactive (total phenolic and total flavonoid) contents and UHPLC-MS secondary metabolites analysis. For phytopharmacological evaluation, in vitro antioxidant (including DPPH, ABTS, FRAP, CUPRAC, phosphomolybdenum, and metal chelation antioxidant assays) activities, enzyme inhibition potential (against AChE, BChE, α-amylase, and tyrosinase enzymes), and in vivo wound healing potential were determined. The tested extract has been shown to contain considerable flavonoid (46.75 mg RE/g extract) and phenolic (33.71 mg GAE/g extract) contents. The plant extract presented considerable antioxidant potential, being the most active for CUPRAC assays. Secondary metabolite UHPLC-MS characterization, in both the positive and negative ionization modes, indicated the tentative presence of 17 different phytocompounds, mostly derivatives of sesquiterpene, alkaloids, and flavonoids. Similarly, the tested extract exhibited considerable inhibitory effects on tyrosinase (81.72 mg KAE/g extract), whereas it showed weak inhibition ability against other tested enzymes. Moreover, in the case of in vivo wound healing assays, significant improvement in wound healing was observed in both the tested models at the doses of 0.5 percent w/w (p < 0.001) and 2.0 percent w/w (p < 0.01) on the 16th day. The outcomes of the present research work suggested that C. gigantea (L.) Dryand plant extract could be appraised as a potential origin of bioactive molecules having multifunctional medicinal uses.
topic Calotropis gigantea
phytochemicals
phenolic content
antioxidant
enzyme inhibition
wound healing
url https://www.frontiersin.org/articles/10.3389/fphar.2021.701369/full
work_keys_str_mv AT ahmedalafnan evaluationofthephytochemicalantioxidantenzymeinhibitionandwoundhealingpotentialofcalotropisgigantealdryandasourceofabioactivemedicinalproduct
AT swathisridharagatta evaluationofthephytochemicalantioxidantenzymeinhibitionandwoundhealingpotentialofcalotropisgigantealdryandasourceofabioactivemedicinalproduct
AT hammadsaleem evaluationofthephytochemicalantioxidantenzymeinhibitionandwoundhealingpotentialofcalotropisgigantealdryandasourceofabioactivemedicinalproduct
AT hammadsaleem evaluationofthephytochemicalantioxidantenzymeinhibitionandwoundhealingpotentialofcalotropisgigantealdryandasourceofabioactivemedicinalproduct
AT umairkhurshid evaluationofthephytochemicalantioxidantenzymeinhibitionandwoundhealingpotentialofcalotropisgigantealdryandasourceofabioactivemedicinalproduct
AT abdulwahabalamri evaluationofthephytochemicalantioxidantenzymeinhibitionandwoundhealingpotentialofcalotropisgigantealdryandasourceofabioactivemedicinalproduct
AT shabanayasmeenansari evaluationofthephytochemicalantioxidantenzymeinhibitionandwoundhealingpotentialofcalotropisgigantealdryandasourceofabioactivemedicinalproduct
AT syafiqasnawizainalabidin evaluationofthephytochemicalantioxidantenzymeinhibitionandwoundhealingpotentialofcalotropisgigantealdryandasourceofabioactivemedicinalproduct
AT siddiqueakberansari evaluationofthephytochemicalantioxidantenzymeinhibitionandwoundhealingpotentialofcalotropisgigantealdryandasourceofabioactivemedicinalproduct
AT abdulhakeemsalamri evaluationofthephytochemicalantioxidantenzymeinhibitionandwoundhealingpotentialofcalotropisgigantealdryandasourceofabioactivemedicinalproduct
AT nafeesahemad evaluationofthephytochemicalantioxidantenzymeinhibitionandwoundhealingpotentialofcalotropisgigantealdryandasourceofabioactivemedicinalproduct
AT sirajudheenanwar evaluationofthephytochemicalantioxidantenzymeinhibitionandwoundhealingpotentialofcalotropisgigantealdryandasourceofabioactivemedicinalproduct
_version_ 1721205093762596864