Determination of Echinococcus granulosus genotypes in livestock slaughtered in Shush County, Southwest Iran using PCR-RFLP
Echinococosis is a zoonotic disease caused by the larval stages of Echinococcus spp. that occurs in most parts of the world. Herein, we aimed to evaluate the genotypes of isolated hydatid cysts from slaughtered animals in Shush county, southwestern Iran. Totally, 96 hydatid cysts were collected, inc...
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doaj-5431371f9b434257b474c91a215390e62021-09-06T19:22:16ZengSciendoHelminthologia0440-66051336-90832019-09-0156319620110.2478/helm-2019-0023helm-2019-0023Determination of Echinococcus granulosus genotypes in livestock slaughtered in Shush County, Southwest Iran using PCR-RFLPFallahizadeh S.0Arjmand R.1Jelowdar A.2Rafiei A.3Kazemi F.4Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranInfectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranInfectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranInfectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranStudent Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranEchinococosis is a zoonotic disease caused by the larval stages of Echinococcus spp. that occurs in most parts of the world. Herein, we aimed to evaluate the genotypes of isolated hydatid cysts from slaughtered animals in Shush county, southwestern Iran. Totally, 96 hydatid cysts were collected, including 11 buffaloes, 13 cattle, 12 goat and 60 sheep. The PCR was done by a primer pair (BDI and 4s) to amplify ITS1 fragment. Four restriction endonucleases including AluI, HpaII, RsaI, and TaqI were used for RFLP products and enzymatic reactions were electrophoresed. Finally, twenty PCR products were sent for sequencing and phylogenetic tree was drawn with MEGA6. Molecular identification of 96 hydatid cysts demonstrated a distinctive 1000 bp fragment in all samples from four animal hosts. RFLP analysis showed similar digestion patterns in all samples. AluI digestion yielded 800 bp and 200 bp fragments, HpaII digestion made 700 bp and 300 bp fragments and RsaI digestion entailed 655 and 345segments. Moreover, TaqI rendered no digestion pattern on rDNA-ITS1 region. Additionally, E. granulosus sensu stricto (G1-3 complex) was the prevailing genotype in all livestock samples, according to PCR-RFLP and sequencing analyses.https://doi.org/10.2478/helm-2019-0023echinococcus granulosusgenotypeslivestockshush countypcr-rflp |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fallahizadeh S. Arjmand R. Jelowdar A. Rafiei A. Kazemi F. |
spellingShingle |
Fallahizadeh S. Arjmand R. Jelowdar A. Rafiei A. Kazemi F. Determination of Echinococcus granulosus genotypes in livestock slaughtered in Shush County, Southwest Iran using PCR-RFLP Helminthologia echinococcus granulosus genotypes livestock shush county pcr-rflp |
author_facet |
Fallahizadeh S. Arjmand R. Jelowdar A. Rafiei A. Kazemi F. |
author_sort |
Fallahizadeh S. |
title |
Determination of Echinococcus granulosus genotypes in livestock slaughtered in Shush County, Southwest Iran using PCR-RFLP |
title_short |
Determination of Echinococcus granulosus genotypes in livestock slaughtered in Shush County, Southwest Iran using PCR-RFLP |
title_full |
Determination of Echinococcus granulosus genotypes in livestock slaughtered in Shush County, Southwest Iran using PCR-RFLP |
title_fullStr |
Determination of Echinococcus granulosus genotypes in livestock slaughtered in Shush County, Southwest Iran using PCR-RFLP |
title_full_unstemmed |
Determination of Echinococcus granulosus genotypes in livestock slaughtered in Shush County, Southwest Iran using PCR-RFLP |
title_sort |
determination of echinococcus granulosus genotypes in livestock slaughtered in shush county, southwest iran using pcr-rflp |
publisher |
Sciendo |
series |
Helminthologia |
issn |
0440-6605 1336-9083 |
publishDate |
2019-09-01 |
description |
Echinococosis is a zoonotic disease caused by the larval stages of Echinococcus spp. that occurs in most parts of the world. Herein, we aimed to evaluate the genotypes of isolated hydatid cysts from slaughtered animals in Shush county, southwestern Iran. Totally, 96 hydatid cysts were collected, including 11 buffaloes, 13 cattle, 12 goat and 60 sheep. The PCR was done by a primer pair (BDI and 4s) to amplify ITS1 fragment. Four restriction endonucleases including AluI, HpaII, RsaI, and TaqI were used for RFLP products and enzymatic reactions were electrophoresed. Finally, twenty PCR products were sent for sequencing and phylogenetic tree was drawn with MEGA6. Molecular identification of 96 hydatid cysts demonstrated a distinctive 1000 bp fragment in all samples from four animal hosts. RFLP analysis showed similar digestion patterns in all samples. AluI digestion yielded 800 bp and 200 bp fragments, HpaII digestion made 700 bp and 300 bp fragments and RsaI digestion entailed 655 and 345segments. Moreover, TaqI rendered no digestion pattern on rDNA-ITS1 region. Additionally, E. granulosus sensu stricto (G1-3 complex) was the prevailing genotype in all livestock samples, according to PCR-RFLP and sequencing analyses. |
topic |
echinococcus granulosus genotypes livestock shush county pcr-rflp |
url |
https://doi.org/10.2478/helm-2019-0023 |
work_keys_str_mv |
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