Divergence Analyses of Sperm DNA Methylomes between Monozygotic Twin AI Bulls
Semen quality is critical for fertility. However, it is easily influenced by environmental factors and can induce subfertility in the next generations. Here, we aimed to assess the impacts of differentially methylated regions and genes on semen quality and offspring fertility. A specific pair of mon...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2019-09-01
|
Series: | Epigenomes |
Subjects: | |
Online Access: | https://www.mdpi.com/2075-4655/3/4/21 |
id |
doaj-55d24219b6bd465d8cadf887d84eed16 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shuli Liu Siqian Chen Wentao Cai Hongwei Yin Aoxing Liu Yanhua Li George E. Liu Yachun Wang Ying Yu Shengli Zhang |
spellingShingle |
Shuli Liu Siqian Chen Wentao Cai Hongwei Yin Aoxing Liu Yanhua Li George E. Liu Yachun Wang Ying Yu Shengli Zhang Divergence Analyses of Sperm DNA Methylomes between Monozygotic Twin AI Bulls Epigenomes monozygotic twins holstein bull dna methylome differentially methylated regions differentially methylated genes |
author_facet |
Shuli Liu Siqian Chen Wentao Cai Hongwei Yin Aoxing Liu Yanhua Li George E. Liu Yachun Wang Ying Yu Shengli Zhang |
author_sort |
Shuli Liu |
title |
Divergence Analyses of Sperm DNA Methylomes between Monozygotic Twin AI Bulls |
title_short |
Divergence Analyses of Sperm DNA Methylomes between Monozygotic Twin AI Bulls |
title_full |
Divergence Analyses of Sperm DNA Methylomes between Monozygotic Twin AI Bulls |
title_fullStr |
Divergence Analyses of Sperm DNA Methylomes between Monozygotic Twin AI Bulls |
title_full_unstemmed |
Divergence Analyses of Sperm DNA Methylomes between Monozygotic Twin AI Bulls |
title_sort |
divergence analyses of sperm dna methylomes between monozygotic twin ai bulls |
publisher |
MDPI AG |
series |
Epigenomes |
issn |
2075-4655 |
publishDate |
2019-09-01 |
description |
Semen quality is critical for fertility. However, it is easily influenced by environmental factors and can induce subfertility in the next generations. Here, we aimed to assess the impacts of differentially methylated regions and genes on semen quality and offspring fertility. A specific pair of monozygotic (MZ) twin artificial insemination (AI) Holstein bulls with moderately different sperm qualities (Bull1 > Bull2) was used in the study, and each twin bull had produced ~6000 recorded daughters nationwide in China. Using whole genome bisulfite sequencing, we profiled the landscape of the twin bulls’ sperm methylomes, and we observed markedly higher sperm methylation levels in Bull1 than in Bull2. Furthermore, we found 528 differentially methylated regions (DMR) between the MZ twin bulls, which spanned or overlapped with 309 differentially methylated genes (DMG). These DMG were particularly associated with embryo development, organ development, reproduction, and the nervous system. Several DMG were also shown to be differentially expressed in the sperm cells. Moreover, the significant differences in DNA methylation on gene <i>INSL3</i> between the MZ twin bulls were confirmed at three different age points. Our results provided new insights into the impacts of AI bull sperm methylomes on offspring fertility. |
topic |
monozygotic twins holstein bull dna methylome differentially methylated regions differentially methylated genes |
url |
https://www.mdpi.com/2075-4655/3/4/21 |
work_keys_str_mv |
AT shuliliu divergenceanalysesofspermdnamethylomesbetweenmonozygotictwinaibulls AT siqianchen divergenceanalysesofspermdnamethylomesbetweenmonozygotictwinaibulls AT wentaocai divergenceanalysesofspermdnamethylomesbetweenmonozygotictwinaibulls AT hongweiyin divergenceanalysesofspermdnamethylomesbetweenmonozygotictwinaibulls AT aoxingliu divergenceanalysesofspermdnamethylomesbetweenmonozygotictwinaibulls AT yanhuali divergenceanalysesofspermdnamethylomesbetweenmonozygotictwinaibulls AT georgeeliu divergenceanalysesofspermdnamethylomesbetweenmonozygotictwinaibulls AT yachunwang divergenceanalysesofspermdnamethylomesbetweenmonozygotictwinaibulls AT yingyu divergenceanalysesofspermdnamethylomesbetweenmonozygotictwinaibulls AT shenglizhang divergenceanalysesofspermdnamethylomesbetweenmonozygotictwinaibulls |
_version_ |
1724173045503885312 |
spelling |
doaj-55d24219b6bd465d8cadf887d84eed162021-04-02T04:48:50ZengMDPI AGEpigenomes2075-46552019-09-01342110.3390/epigenomes3040021epigenomes3040021Divergence Analyses of Sperm DNA Methylomes between Monozygotic Twin AI BullsShuli Liu0Siqian Chen1Wentao Cai2Hongwei Yin3Aoxing Liu4Yanhua Li5George E. Liu6Yachun Wang7Ying Yu8Shengli Zhang9Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 2rd, Yuanmingyuan West Road, Beijing 100193, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 2rd, Yuanmingyuan West Road, Beijing 100193, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 2rd, Yuanmingyuan West Road, Beijing 100193, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 2rd, Yuanmingyuan West Road, Beijing 100193, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 2rd, Yuanmingyuan West Road, Beijing 100193, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 2rd, Yuanmingyuan West Road, Beijing 100193, ChinaAnimal Genomics and Improvement Laboratory, BARC, USDA-ARS, BARC-East, Beltsville, MD 20705, USAKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 2rd, Yuanmingyuan West Road, Beijing 100193, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 2rd, Yuanmingyuan West Road, Beijing 100193, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture & National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 2rd, Yuanmingyuan West Road, Beijing 100193, ChinaSemen quality is critical for fertility. However, it is easily influenced by environmental factors and can induce subfertility in the next generations. Here, we aimed to assess the impacts of differentially methylated regions and genes on semen quality and offspring fertility. A specific pair of monozygotic (MZ) twin artificial insemination (AI) Holstein bulls with moderately different sperm qualities (Bull1 > Bull2) was used in the study, and each twin bull had produced ~6000 recorded daughters nationwide in China. Using whole genome bisulfite sequencing, we profiled the landscape of the twin bulls’ sperm methylomes, and we observed markedly higher sperm methylation levels in Bull1 than in Bull2. Furthermore, we found 528 differentially methylated regions (DMR) between the MZ twin bulls, which spanned or overlapped with 309 differentially methylated genes (DMG). These DMG were particularly associated with embryo development, organ development, reproduction, and the nervous system. Several DMG were also shown to be differentially expressed in the sperm cells. Moreover, the significant differences in DNA methylation on gene <i>INSL3</i> between the MZ twin bulls were confirmed at three different age points. Our results provided new insights into the impacts of AI bull sperm methylomes on offspring fertility.https://www.mdpi.com/2075-4655/3/4/21monozygotic twinsholstein bulldna methylomedifferentially methylated regionsdifferentially methylated genes |