Sequencing of the rus gene before and after the mutation with DES in the bacterial Acidithiobacillus sp. FJ2
In Acidithiobacillus ferrooxidans, the proteins present in the electron transfer pathway cause ferrous iron oxidation which leads to uranium extraction. The relationship between gene sequence and uranium extraction has not been investigated yet. Based on the changes in uranium extraction, the change...
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Kharazmi University
2019-05-01
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doaj-56559e17787945e6a0300591c5dcca6c2020-11-24T20:43:41ZengKharazmi Universityیافتههای نوین در علوم زیستی2423-63302476-71152019-05-01615060Sequencing of the rus gene before and after the mutation with DES in the bacterial Acidithiobacillus sp. FJ2Somayeh Farahmand0Faezeh Fatemi1Reza Hajihosseini2 payame noor University NSTRI payame noor University In Acidithiobacillus ferrooxidans, the proteins present in the electron transfer pathway cause ferrous iron oxidation which leads to uranium extraction. The relationship between gene sequence and uranium extraction has not been investigated yet. Based on the changes in uranium extraction, the changes of rus gene sequence can reveal the direct and accurate role of this protein. For this purpose, a random mutation was induced in native Acidithiobacillus sp. FJ2 by two doses of 0.8% and 1% of DES. Then, the bacteria was transferred into a medium which contained 50% uranium ore to carry out the bioleaching process. After measuring the amount of the extracted uranium, iron, Eh and pH, genomic DNA was extracted to investigate the rusticyanin gene (rus) sequence sent for sequencing after performing PCR. Then, the wild-type gene sequence was compared with the mutant by Bioedit v7.2.5 software. The results showed that uranium extraction increased by mutant bacteria with DES 1% between 7-11 days in comparison with wild bacteria. However, there has been no change in the functional areas of the rusticyanin gene. It seems that DES affected other effective genes in the electron transport chain or regulatory areas, which required further studies.http://nbr.khu.ac.ir/browse.php?a_code=A-10-114-2&slc_lang=en&sid=1bioleachingelectron transportmicroorganismproteinrandom mutation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Somayeh Farahmand Faezeh Fatemi Reza Hajihosseini |
spellingShingle |
Somayeh Farahmand Faezeh Fatemi Reza Hajihosseini Sequencing of the rus gene before and after the mutation with DES in the bacterial Acidithiobacillus sp. FJ2 یافتههای نوین در علوم زیستی bioleaching electron transport microorganism protein random mutation |
author_facet |
Somayeh Farahmand Faezeh Fatemi Reza Hajihosseini |
author_sort |
Somayeh Farahmand |
title |
Sequencing of the rus gene before and after the mutation with DES in the bacterial Acidithiobacillus sp. FJ2 |
title_short |
Sequencing of the rus gene before and after the mutation with DES in the bacterial Acidithiobacillus sp. FJ2 |
title_full |
Sequencing of the rus gene before and after the mutation with DES in the bacterial Acidithiobacillus sp. FJ2 |
title_fullStr |
Sequencing of the rus gene before and after the mutation with DES in the bacterial Acidithiobacillus sp. FJ2 |
title_full_unstemmed |
Sequencing of the rus gene before and after the mutation with DES in the bacterial Acidithiobacillus sp. FJ2 |
title_sort |
sequencing of the rus gene before and after the mutation with des in the bacterial acidithiobacillus sp. fj2 |
publisher |
Kharazmi University |
series |
یافتههای نوین در علوم زیستی |
issn |
2423-6330 2476-7115 |
publishDate |
2019-05-01 |
description |
In Acidithiobacillus ferrooxidans, the proteins present in the electron transfer pathway cause ferrous iron oxidation which leads to uranium extraction. The relationship between gene sequence and uranium extraction has not been investigated yet. Based on the changes in uranium extraction, the changes of rus gene sequence can reveal the direct and accurate role of this protein. For this purpose, a random mutation was induced in native Acidithiobacillus sp. FJ2 by two doses of 0.8% and 1% of DES. Then, the bacteria was transferred into a medium which contained 50% uranium ore to carry out the bioleaching process. After measuring the amount of the extracted uranium, iron, Eh and pH, genomic DNA was extracted to investigate the rusticyanin gene (rus) sequence sent for sequencing after performing PCR. Then, the wild-type gene sequence was compared with the mutant by Bioedit v7.2.5 software. The results showed that uranium extraction increased by mutant bacteria with DES 1% between 7-11 days in comparison with wild bacteria. However, there has been no change in the functional areas of the rusticyanin gene. It seems that DES affected other effective genes in the electron transport chain or regulatory areas, which required further studies. |
topic |
bioleaching electron transport microorganism protein random mutation |
url |
http://nbr.khu.ac.ir/browse.php?a_code=A-10-114-2&slc_lang=en&sid=1 |
work_keys_str_mv |
AT somayehfarahmand sequencingoftherusgenebeforeandafterthemutationwithdesinthebacterialacidithiobacillusspfj2 AT faezehfatemi sequencingoftherusgenebeforeandafterthemutationwithdesinthebacterialacidithiobacillusspfj2 AT rezahajihosseini sequencingoftherusgenebeforeandafterthemutationwithdesinthebacterialacidithiobacillusspfj2 |
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1716819144046804992 |