A high-efficiency method for site-directed mutagenesis of large plasmids based on large DNA fragment amplification and recombinational ligation

Abstract Site-directed mutagenesis for large plasmids is a difficult task that cannot easily be solved by the conventional methods used in many laboratories. In this study, we developed an effective method for Site-directed Mutagenesis for Large Plasmids (SMLP) based on a PCR technique. The SMLP met...

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Bibliographic Details
Main Authors: Kewei Zhang, Xiaomei Yin, Kaituo Shi, Shihua Zhang, Juan Wang, Shasha Zhao, Huan Deng, Cheng Zhang, Zihui Wu, Yuan Li, Xiangyu Zhou, Wensheng Deng
Format: Article
Language:English
Published: Nature Publishing Group 2021-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-89884-z