Geographic Distribution of Leishmania Species in Ecuador Based on the Cytochrome B Gene Sequence Analysis.
A countrywide epidemiological study was performed to elucidate the current geographic distribution of causative species of cutaneous leishmaniasis (CL) in Ecuador by using FTA card-spotted samples and smear slides as DNA sources. Putative Leishmania in 165 samples collected from patients with CL in...
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2016-07-01
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Series: | PLoS Neglected Tropical Diseases |
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doaj-5808b5403b834fc2a4ecf14fe184accb2020-11-25T01:45:50ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352016-07-01107e000484410.1371/journal.pntd.0004844Geographic Distribution of Leishmania Species in Ecuador Based on the Cytochrome B Gene Sequence Analysis.Hirotomo KatoEduardo A GomezLuiggi Martini-RoblesJenny MuzzioLenin VelezManuel CalvopiñaDaniel Romero-AlvarezTatsuyuki MimoriHiroshi UezatoYoshihisa HashiguchiA countrywide epidemiological study was performed to elucidate the current geographic distribution of causative species of cutaneous leishmaniasis (CL) in Ecuador by using FTA card-spotted samples and smear slides as DNA sources. Putative Leishmania in 165 samples collected from patients with CL in 16 provinces of Ecuador were examined at the species level based on the cytochrome b gene sequence analysis. Of these, 125 samples were successfully identified as Leishmania (Viannia) guyanensis, L. (V.) braziliensis, L. (V.) naiffi, L. (V.) lainsoni, and L. (Leishmania) mexicana. Two dominant species, L. (V.) guyanensis and L. (V.) braziliensis, were widely distributed in Pacific coast subtropical and Amazonian tropical areas, respectively. Recently reported L. (V.) naiffi and L. (V.) lainsoni were identified in Amazonian areas, and L. (L.) mexicana was identified in an Andean highland area. Importantly, the present study demonstrated that cases of L. (V.) braziliensis infection are increasing in Pacific coast areas.http://europepmc.org/articles/PMC4943627?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hirotomo Kato Eduardo A Gomez Luiggi Martini-Robles Jenny Muzzio Lenin Velez Manuel Calvopiña Daniel Romero-Alvarez Tatsuyuki Mimori Hiroshi Uezato Yoshihisa Hashiguchi |
spellingShingle |
Hirotomo Kato Eduardo A Gomez Luiggi Martini-Robles Jenny Muzzio Lenin Velez Manuel Calvopiña Daniel Romero-Alvarez Tatsuyuki Mimori Hiroshi Uezato Yoshihisa Hashiguchi Geographic Distribution of Leishmania Species in Ecuador Based on the Cytochrome B Gene Sequence Analysis. PLoS Neglected Tropical Diseases |
author_facet |
Hirotomo Kato Eduardo A Gomez Luiggi Martini-Robles Jenny Muzzio Lenin Velez Manuel Calvopiña Daniel Romero-Alvarez Tatsuyuki Mimori Hiroshi Uezato Yoshihisa Hashiguchi |
author_sort |
Hirotomo Kato |
title |
Geographic Distribution of Leishmania Species in Ecuador Based on the Cytochrome B Gene Sequence Analysis. |
title_short |
Geographic Distribution of Leishmania Species in Ecuador Based on the Cytochrome B Gene Sequence Analysis. |
title_full |
Geographic Distribution of Leishmania Species in Ecuador Based on the Cytochrome B Gene Sequence Analysis. |
title_fullStr |
Geographic Distribution of Leishmania Species in Ecuador Based on the Cytochrome B Gene Sequence Analysis. |
title_full_unstemmed |
Geographic Distribution of Leishmania Species in Ecuador Based on the Cytochrome B Gene Sequence Analysis. |
title_sort |
geographic distribution of leishmania species in ecuador based on the cytochrome b gene sequence analysis. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Neglected Tropical Diseases |
issn |
1935-2727 1935-2735 |
publishDate |
2016-07-01 |
description |
A countrywide epidemiological study was performed to elucidate the current geographic distribution of causative species of cutaneous leishmaniasis (CL) in Ecuador by using FTA card-spotted samples and smear slides as DNA sources. Putative Leishmania in 165 samples collected from patients with CL in 16 provinces of Ecuador were examined at the species level based on the cytochrome b gene sequence analysis. Of these, 125 samples were successfully identified as Leishmania (Viannia) guyanensis, L. (V.) braziliensis, L. (V.) naiffi, L. (V.) lainsoni, and L. (Leishmania) mexicana. Two dominant species, L. (V.) guyanensis and L. (V.) braziliensis, were widely distributed in Pacific coast subtropical and Amazonian tropical areas, respectively. Recently reported L. (V.) naiffi and L. (V.) lainsoni were identified in Amazonian areas, and L. (L.) mexicana was identified in an Andean highland area. Importantly, the present study demonstrated that cases of L. (V.) braziliensis infection are increasing in Pacific coast areas. |
url |
http://europepmc.org/articles/PMC4943627?pdf=render |
work_keys_str_mv |
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