Identification and purification of human induced pluripotent stem cell-derived atrial-like cardiomyocytes based on sarcolipin expression.
The use of human stem cell-derived cardiomyocytes to study atrial biology and disease has been restricted by the lack of a reliable method for stem cell-derived atrial cell labeling and purification. The goal of this study was to generate an atrial-specific reporter construct to identify and purify...
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doaj-5826540853ae47d1bb19e0620eb13a832020-11-24T22:06:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0197e10131610.1371/journal.pone.0101316Identification and purification of human induced pluripotent stem cell-derived atrial-like cardiomyocytes based on sarcolipin expression.Rebecca JosowitzJia LuChristine FalceSunita L D'SouzaMeng WuNinette CohenNicole C DuboisYong ZhaoEric A SobieGlenn I FishmanBruce D GelbThe use of human stem cell-derived cardiomyocytes to study atrial biology and disease has been restricted by the lack of a reliable method for stem cell-derived atrial cell labeling and purification. The goal of this study was to generate an atrial-specific reporter construct to identify and purify human stem cell-derived atrial-like cardiomyocytes. We have created a bacterial artificial chromosome (BAC) reporter construct in which fluorescence is driven by expression of the atrial-specific gene sarcolipin (SLN). When purified using flow cytometry, cells with high fluorescence specifically express atrial genes and display functional calcium handling and electrophysiological properties consistent with atrial cardiomyocytes. Our data indicate that SLN can be used as a marker to successfully monitor and isolate hiPSC-derived atrial-like cardiomyocytes. These purified cells may find many applications, including in the study of atrial-specific pathologies and chamber-specific lineage development.http://europepmc.org/articles/PMC4092021?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rebecca Josowitz Jia Lu Christine Falce Sunita L D'Souza Meng Wu Ninette Cohen Nicole C Dubois Yong Zhao Eric A Sobie Glenn I Fishman Bruce D Gelb |
spellingShingle |
Rebecca Josowitz Jia Lu Christine Falce Sunita L D'Souza Meng Wu Ninette Cohen Nicole C Dubois Yong Zhao Eric A Sobie Glenn I Fishman Bruce D Gelb Identification and purification of human induced pluripotent stem cell-derived atrial-like cardiomyocytes based on sarcolipin expression. PLoS ONE |
author_facet |
Rebecca Josowitz Jia Lu Christine Falce Sunita L D'Souza Meng Wu Ninette Cohen Nicole C Dubois Yong Zhao Eric A Sobie Glenn I Fishman Bruce D Gelb |
author_sort |
Rebecca Josowitz |
title |
Identification and purification of human induced pluripotent stem cell-derived atrial-like cardiomyocytes based on sarcolipin expression. |
title_short |
Identification and purification of human induced pluripotent stem cell-derived atrial-like cardiomyocytes based on sarcolipin expression. |
title_full |
Identification and purification of human induced pluripotent stem cell-derived atrial-like cardiomyocytes based on sarcolipin expression. |
title_fullStr |
Identification and purification of human induced pluripotent stem cell-derived atrial-like cardiomyocytes based on sarcolipin expression. |
title_full_unstemmed |
Identification and purification of human induced pluripotent stem cell-derived atrial-like cardiomyocytes based on sarcolipin expression. |
title_sort |
identification and purification of human induced pluripotent stem cell-derived atrial-like cardiomyocytes based on sarcolipin expression. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
The use of human stem cell-derived cardiomyocytes to study atrial biology and disease has been restricted by the lack of a reliable method for stem cell-derived atrial cell labeling and purification. The goal of this study was to generate an atrial-specific reporter construct to identify and purify human stem cell-derived atrial-like cardiomyocytes. We have created a bacterial artificial chromosome (BAC) reporter construct in which fluorescence is driven by expression of the atrial-specific gene sarcolipin (SLN). When purified using flow cytometry, cells with high fluorescence specifically express atrial genes and display functional calcium handling and electrophysiological properties consistent with atrial cardiomyocytes. Our data indicate that SLN can be used as a marker to successfully monitor and isolate hiPSC-derived atrial-like cardiomyocytes. These purified cells may find many applications, including in the study of atrial-specific pathologies and chamber-specific lineage development. |
url |
http://europepmc.org/articles/PMC4092021?pdf=render |
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