The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1

Lantibiotics are (methyl)-lanthionine-containing antimicrobial peptides produced by several Gram-positive bacteria. Some human pathogenic bacteria express specific resistance proteins that counteract this antimicrobial activity of lantibiotics. In Streptococcus agalactiae COH1 resistance against the...

Full description

Bibliographic Details
Main Authors: Jens Reiners, Marcel Lagedroste, Katja Ehlen, Selina Leusch, Julia Zaschke-Kriesche, Sander H. J. Smits
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-08-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2017.01643/full
id doaj-58e5396419914b04bf4199d6f83cdfad
record_format Article
spelling doaj-58e5396419914b04bf4199d6f83cdfad2020-11-25T00:03:22ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-08-01810.3389/fmicb.2017.01643276395The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1Jens ReinersMarcel LagedrosteKatja EhlenSelina LeuschJulia Zaschke-KriescheSander H. J. SmitsLantibiotics are (methyl)-lanthionine-containing antimicrobial peptides produced by several Gram-positive bacteria. Some human pathogenic bacteria express specific resistance proteins that counteract this antimicrobial activity of lantibiotics. In Streptococcus agalactiae COH1 resistance against the well-known lantibiotic nisin is conferred by, the nisin resistance protein (NSR), a two-component system (NsrRK) and a BceAB-type ATP-binding cassette (ABC) transporter (NsrFP). The present study focuses on elucidating the function of NsrFP via its heterologous expression in Lactococcus lactis. NsrFP is able to confer a 16-fold resistance against wild type nisin as determined by growth inhibition experiments and functions as a lantibiotic exporter. Several C-terminal nisin mutants indicated that NsrFP recognizes the N-terminal region of nisin. The N-terminus harbors three (methyl)-lanthionine rings, which are conserved in other lantibiotics.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01643/fullABC transporterlanthionine ringlantibioticnisinresistance
collection DOAJ
language English
format Article
sources DOAJ
author Jens Reiners
Marcel Lagedroste
Katja Ehlen
Selina Leusch
Julia Zaschke-Kriesche
Sander H. J. Smits
spellingShingle Jens Reiners
Marcel Lagedroste
Katja Ehlen
Selina Leusch
Julia Zaschke-Kriesche
Sander H. J. Smits
The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1
Frontiers in Microbiology
ABC transporter
lanthionine ring
lantibiotic
nisin
resistance
author_facet Jens Reiners
Marcel Lagedroste
Katja Ehlen
Selina Leusch
Julia Zaschke-Kriesche
Sander H. J. Smits
author_sort Jens Reiners
title The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1
title_short The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1
title_full The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1
title_fullStr The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1
title_full_unstemmed The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1
title_sort n-terminal region of nisin is important for the bceab-type abc transporter nsrfp from streptococcus agalactiae coh1
publisher Frontiers Media S.A.
series Frontiers in Microbiology
issn 1664-302X
publishDate 2017-08-01
description Lantibiotics are (methyl)-lanthionine-containing antimicrobial peptides produced by several Gram-positive bacteria. Some human pathogenic bacteria express specific resistance proteins that counteract this antimicrobial activity of lantibiotics. In Streptococcus agalactiae COH1 resistance against the well-known lantibiotic nisin is conferred by, the nisin resistance protein (NSR), a two-component system (NsrRK) and a BceAB-type ATP-binding cassette (ABC) transporter (NsrFP). The present study focuses on elucidating the function of NsrFP via its heterologous expression in Lactococcus lactis. NsrFP is able to confer a 16-fold resistance against wild type nisin as determined by growth inhibition experiments and functions as a lantibiotic exporter. Several C-terminal nisin mutants indicated that NsrFP recognizes the N-terminal region of nisin. The N-terminus harbors three (methyl)-lanthionine rings, which are conserved in other lantibiotics.
topic ABC transporter
lanthionine ring
lantibiotic
nisin
resistance
url http://journal.frontiersin.org/article/10.3389/fmicb.2017.01643/full
work_keys_str_mv AT jensreiners thenterminalregionofnisinisimportantforthebceabtypeabctransporternsrfpfromstreptococcusagalactiaecoh1
AT marcellagedroste thenterminalregionofnisinisimportantforthebceabtypeabctransporternsrfpfromstreptococcusagalactiaecoh1
AT katjaehlen thenterminalregionofnisinisimportantforthebceabtypeabctransporternsrfpfromstreptococcusagalactiaecoh1
AT selinaleusch thenterminalregionofnisinisimportantforthebceabtypeabctransporternsrfpfromstreptococcusagalactiaecoh1
AT juliazaschkekriesche thenterminalregionofnisinisimportantforthebceabtypeabctransporternsrfpfromstreptococcusagalactiaecoh1
AT sanderhjsmits thenterminalregionofnisinisimportantforthebceabtypeabctransporternsrfpfromstreptococcusagalactiaecoh1
AT jensreiners nterminalregionofnisinisimportantforthebceabtypeabctransporternsrfpfromstreptococcusagalactiaecoh1
AT marcellagedroste nterminalregionofnisinisimportantforthebceabtypeabctransporternsrfpfromstreptococcusagalactiaecoh1
AT katjaehlen nterminalregionofnisinisimportantforthebceabtypeabctransporternsrfpfromstreptococcusagalactiaecoh1
AT selinaleusch nterminalregionofnisinisimportantforthebceabtypeabctransporternsrfpfromstreptococcusagalactiaecoh1
AT juliazaschkekriesche nterminalregionofnisinisimportantforthebceabtypeabctransporternsrfpfromstreptococcusagalactiaecoh1
AT sanderhjsmits nterminalregionofnisinisimportantforthebceabtypeabctransporternsrfpfromstreptococcusagalactiaecoh1
_version_ 1725434443012767744