The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1
Lantibiotics are (methyl)-lanthionine-containing antimicrobial peptides produced by several Gram-positive bacteria. Some human pathogenic bacteria express specific resistance proteins that counteract this antimicrobial activity of lantibiotics. In Streptococcus agalactiae COH1 resistance against the...
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2017-08-01
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doaj-58e5396419914b04bf4199d6f83cdfad2020-11-25T00:03:22ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-08-01810.3389/fmicb.2017.01643276395The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1Jens ReinersMarcel LagedrosteKatja EhlenSelina LeuschJulia Zaschke-KriescheSander H. J. SmitsLantibiotics are (methyl)-lanthionine-containing antimicrobial peptides produced by several Gram-positive bacteria. Some human pathogenic bacteria express specific resistance proteins that counteract this antimicrobial activity of lantibiotics. In Streptococcus agalactiae COH1 resistance against the well-known lantibiotic nisin is conferred by, the nisin resistance protein (NSR), a two-component system (NsrRK) and a BceAB-type ATP-binding cassette (ABC) transporter (NsrFP). The present study focuses on elucidating the function of NsrFP via its heterologous expression in Lactococcus lactis. NsrFP is able to confer a 16-fold resistance against wild type nisin as determined by growth inhibition experiments and functions as a lantibiotic exporter. Several C-terminal nisin mutants indicated that NsrFP recognizes the N-terminal region of nisin. The N-terminus harbors three (methyl)-lanthionine rings, which are conserved in other lantibiotics.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01643/fullABC transporterlanthionine ringlantibioticnisinresistance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jens Reiners Marcel Lagedroste Katja Ehlen Selina Leusch Julia Zaschke-Kriesche Sander H. J. Smits |
spellingShingle |
Jens Reiners Marcel Lagedroste Katja Ehlen Selina Leusch Julia Zaschke-Kriesche Sander H. J. Smits The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1 Frontiers in Microbiology ABC transporter lanthionine ring lantibiotic nisin resistance |
author_facet |
Jens Reiners Marcel Lagedroste Katja Ehlen Selina Leusch Julia Zaschke-Kriesche Sander H. J. Smits |
author_sort |
Jens Reiners |
title |
The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1 |
title_short |
The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1 |
title_full |
The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1 |
title_fullStr |
The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1 |
title_full_unstemmed |
The N-terminal Region of Nisin Is Important for the BceAB-Type ABC Transporter NsrFP from Streptococcus agalactiae COH1 |
title_sort |
n-terminal region of nisin is important for the bceab-type abc transporter nsrfp from streptococcus agalactiae coh1 |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2017-08-01 |
description |
Lantibiotics are (methyl)-lanthionine-containing antimicrobial peptides produced by several Gram-positive bacteria. Some human pathogenic bacteria express specific resistance proteins that counteract this antimicrobial activity of lantibiotics. In Streptococcus agalactiae COH1 resistance against the well-known lantibiotic nisin is conferred by, the nisin resistance protein (NSR), a two-component system (NsrRK) and a BceAB-type ATP-binding cassette (ABC) transporter (NsrFP). The present study focuses on elucidating the function of NsrFP via its heterologous expression in Lactococcus lactis. NsrFP is able to confer a 16-fold resistance against wild type nisin as determined by growth inhibition experiments and functions as a lantibiotic exporter. Several C-terminal nisin mutants indicated that NsrFP recognizes the N-terminal region of nisin. The N-terminus harbors three (methyl)-lanthionine rings, which are conserved in other lantibiotics. |
topic |
ABC transporter lanthionine ring lantibiotic nisin resistance |
url |
http://journal.frontiersin.org/article/10.3389/fmicb.2017.01643/full |
work_keys_str_mv |
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