A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification

A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification

As a result of its being inexpensive, easy to perform, fast and accurate, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) is quickly becoming the standard means of bacterial identification from cultures in clinical microbiology laboratories. Its adoption f...

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Bibliographic Details
Main Author: Robin Patel
Format: Article
Language:English
Published: MDPI AG 2019-01-01
Series:Journal of Fungi
Subjects:
MALDI-ToF MS
yeast
fungus
Online Access:http://www.mdpi.com/2309-608X/5/1/4
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spelling doaj-59848d4eccb14d7a9ed75a5acb486f2f2020-11-24T22:53:41ZengMDPI AGJournal of Fungi2309-608X2019-01-0151410.3390/jof5010004jof5010004A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal IdentificationRobin Patel0Department of Laboratory Medicine and Pathology, Division of Clinical Microbiology, Mayo Clinic, Rochester, MN 55905, USAAs a result of its being inexpensive, easy to perform, fast and accurate, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) is quickly becoming the standard means of bacterial identification from cultures in clinical microbiology laboratories. Its adoption for routine identification of yeasts and even dimorphic and filamentous fungi in cultures, while slower, is now being realized, with many of the same benefits as have been recognized on the bacterial side. In this review, the use of MALDI-ToF MS for identification of yeasts, and dimorphic and filamentous fungi grown in culture will be reviewed, with strengths and limitations addressed.http://www.mdpi.com/2309-608X/5/1/4MALDI-ToF MSyeastfungus
collection DOAJ
language English
format Article
sources DOAJ
author Robin Patel
spellingShingle Robin Patel
A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification
Journal of Fungi
MALDI-ToF MS
yeast
fungus
author_facet Robin Patel
author_sort Robin Patel
title A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification
title_short A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification
title_full A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification
title_fullStr A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification
title_full_unstemmed A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification
title_sort moldy application of maldi: maldi-tof mass spectrometry for fungal identification
publisher MDPI AG
series Journal of Fungi
issn 2309-608X
publishDate 2019-01-01
description As a result of its being inexpensive, easy to perform, fast and accurate, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) is quickly becoming the standard means of bacterial identification from cultures in clinical microbiology laboratories. Its adoption for routine identification of yeasts and even dimorphic and filamentous fungi in cultures, while slower, is now being realized, with many of the same benefits as have been recognized on the bacterial side. In this review, the use of MALDI-ToF MS for identification of yeasts, and dimorphic and filamentous fungi grown in culture will be reviewed, with strengths and limitations addressed.
topic MALDI-ToF MS
yeast
fungus
url http://www.mdpi.com/2309-608X/5/1/4
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