A fast universal immobilization of immunoglobulin G at 4 °C for the development of array-based immunoassays.

To maintain the antibody activity and enhance performance of array-based immunoassays, protein G was used to allow a shorter duration of immunoglobulin G immobilization at 4 °C, with the antibody placed in the appropriate orientation. The multiplexed detection of six pain-related message molecules (...

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Bibliographic Details
Main Authors: Shu-Lin Guo, Po-Chung Chen, Ming-Shuo Chen, Yu-Che Cheng, Jun-Mu Lin, Hoong-Chien Lee, Chien-Sheng Chen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3517563?pdf=render