A protocol for simultaneous Ca2+ and morphology imaging of brain endothelial tip cells in larval zebrafish

Summary: Endothelial tip cells (ETCs) located at growing blood vessels display high morphological dynamics and associated intracellular Ca2+ activities with different spatiotemporal patterns during migration. Examining the Ca2+ activity and morphological dynamics of ETCs will provide an insight for...

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Bibliographic Details
Main Authors: Ting-ting Liu, Han Hou, Jiu-lin Du
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000952