Efficient Biolistic Transformation of Immature Citrus Rootstocks Using Phosphomannose-isomerase Selection

This research utilized the <i>E. coli man</i>A gene encoding phosphomannose isomerase (PMI) selection on sucrose/mannose medium to increase transformation efficiencies after biolistic transformation of two immature citrus rootstock cultivars. Plasmid DNA, containing the <i>man</...

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Bibliographic Details
Main Authors: Hao Wu, Yosvanis Acanda, Michel Canton, Janice Zale
Format: Article
Published: MDPI AG 2019-09-01
Online Access:https://www.mdpi.com/2223-7747/8/10/390