Regeneration of oat androgenic plants in relation to induction media and culture conditions of embryo-like structures
The effect of C17 and W14 induction media on the formation of embryo-like structures (ELS) from F3 generation of nine hexaploid oat hybrids was investigated in the study. In all genotypes, the highest number of ELS (0.6 - 12.1/100 anthers) was obtained on C17 medium. The efficiency of plant regenera...
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Polish Botanical Society
2011-01-01
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doaj-5fc93fc90d974b70a5662e11b8fbb8162020-11-25T03:21:26ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802011-01-0178320921310.5586/asbp.2009.026202Regeneration of oat androgenic plants in relation to induction media and culture conditions of embryo-like structuresAleksandra Ponitka0Aurelia Ślusarkiewicz-Jarzina1Polish Academy of SciencesPolish Academy of SciencesThe effect of C17 and W14 induction media on the formation of embryo-like structures (ELS) from F3 generation of nine hexaploid oat hybrids was investigated in the study. In all genotypes, the highest number of ELS (0.6 - 12.1/100 anthers) was obtained on C17 medium. The efficiency of plant regeneration on medium 190-2 was tested, in relation to different ELS culture conditions. The highest rate of green plants per 100 ELS (3.3 - 42.4) was produced by incubation at 22oC in the dark for the first two weeks. Among 36 green regenerants, 28 (77.8%) were haploid and 8 (22.2%) were spontaneous doubled haploids, fully fertile. After colchicine treatment of haploid plants, 19 were partially fertile and set from 1 to 15 seed per panicle.https://pbsociety.org.pl/journals/index.php/asbp/article/view/390anther cultureAvena sativaculture conditionsdoubled haploidsembryo-like structureshaploid plants |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aleksandra Ponitka Aurelia Ślusarkiewicz-Jarzina |
spellingShingle |
Aleksandra Ponitka Aurelia Ślusarkiewicz-Jarzina Regeneration of oat androgenic plants in relation to induction media and culture conditions of embryo-like structures Acta Societatis Botanicorum Poloniae anther culture Avena sativa culture conditions doubled haploids embryo-like structures haploid plants |
author_facet |
Aleksandra Ponitka Aurelia Ślusarkiewicz-Jarzina |
author_sort |
Aleksandra Ponitka |
title |
Regeneration of oat androgenic plants in relation to induction media and culture conditions of embryo-like structures |
title_short |
Regeneration of oat androgenic plants in relation to induction media and culture conditions of embryo-like structures |
title_full |
Regeneration of oat androgenic plants in relation to induction media and culture conditions of embryo-like structures |
title_fullStr |
Regeneration of oat androgenic plants in relation to induction media and culture conditions of embryo-like structures |
title_full_unstemmed |
Regeneration of oat androgenic plants in relation to induction media and culture conditions of embryo-like structures |
title_sort |
regeneration of oat androgenic plants in relation to induction media and culture conditions of embryo-like structures |
publisher |
Polish Botanical Society |
series |
Acta Societatis Botanicorum Poloniae |
issn |
2083-9480 |
publishDate |
2011-01-01 |
description |
The effect of C17 and W14 induction media on the formation of embryo-like structures (ELS) from F3 generation of nine hexaploid oat hybrids was investigated in the study. In all genotypes, the highest number of ELS (0.6 - 12.1/100 anthers) was obtained on C17 medium. The efficiency of plant regeneration on medium 190-2 was tested, in relation to different ELS culture conditions. The highest rate of green plants per 100 ELS (3.3 - 42.4) was produced by incubation at 22oC in the dark for the first two weeks. Among 36 green regenerants, 28 (77.8%) were haploid and 8 (22.2%) were spontaneous doubled haploids, fully fertile. After colchicine treatment of haploid plants, 19 were partially fertile and set from 1 to 15 seed per panicle. |
topic |
anther culture Avena sativa culture conditions doubled haploids embryo-like structures haploid plants |
url |
https://pbsociety.org.pl/journals/index.php/asbp/article/view/390 |
work_keys_str_mv |
AT aleksandraponitka regenerationofoatandrogenicplantsinrelationtoinductionmediaandcultureconditionsofembryolikestructures AT aureliaslusarkiewiczjarzina regenerationofoatandrogenicplantsinrelationtoinductionmediaandcultureconditionsofembryolikestructures |
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